Proteic Feeder Effect on Glucose Oxidase Aggregates Formation
Abstract
In this study, it is aimed to prepare cross-linked glucose oxidase GOD aggregates by using different proteic feeders.Bovine serum albumin BSA , gelatine and collagen were used separately as proteic feeders in the aggregate preparation process. The initial enzyme concentration was kept constant as 0.05 mg/ml. The amount of BSA varied from1 to 50 mg and the amount of gelatine and collagen were in the range of 5- 75 mg. A cross-linker, glutaraldehyde 2% v/v has been used in order to form GOD aggregates with proteic feeder. The highest immobilization efficiency wasfound when BSA feeder was used. Activities of both free and immobilised GOD were obtained by measuring theamount of hydrogen peroxide formed from glucose conversion, by using spectrophotometer. The maximum activitieswere obtained at following proteic feeder amounts: BSA 5 mg, collagen 60 mg, gelatine 50 mg. Kinetic parametersof native and immobilised enzyme have been calculated by using Lineweaver-Burk plots in the substrate range of0.01-1 M . The maximum reaction rates for free and immobilised enzyme with different proteic feeders, BSA, gelatine and collagen were calculated as 66.29, 12.22, 9.29, 4.53 mM/min-1, respectively. The corresponding saturationconstants of systems with the maximum activity were 7.9, 11.37, 22.58, 33.6 mM, respectively
Keywords
References
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Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
December 1, 2008
Submission Date
-
Acceptance Date
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Published in Issue
Year 2008 Volume: 36 Number: 4
APA
İspirli, Y., & Ayhan, H. (2008). Proteic Feeder Effect on Glucose Oxidase Aggregates Formation. Hacettepe Journal of Biology and Chemistry, 36(4), 313-318. https://izlik.org/JA69YW86LS
AMA
1.İspirli Y, Ayhan H. Proteic Feeder Effect on Glucose Oxidase Aggregates Formation. HJBC. 2008;36(4):313-318. https://izlik.org/JA69YW86LS
Chicago
İspirli, Yasemin, and Hakan Ayhan. 2008. “Proteic Feeder Effect on Glucose Oxidase Aggregates Formation”. Hacettepe Journal of Biology and Chemistry 36 (4): 313-18. https://izlik.org/JA69YW86LS.
EndNote
İspirli Y, Ayhan H (December 1, 2008) Proteic Feeder Effect on Glucose Oxidase Aggregates Formation. Hacettepe Journal of Biology and Chemistry 36 4 313–318.
IEEE
[1]Y. İspirli and H. Ayhan, “Proteic Feeder Effect on Glucose Oxidase Aggregates Formation”, HJBC, vol. 36, no. 4, pp. 313–318, Dec. 2008, [Online]. Available: https://izlik.org/JA69YW86LS
ISNAD
İspirli, Yasemin - Ayhan, Hakan. “Proteic Feeder Effect on Glucose Oxidase Aggregates Formation”. Hacettepe Journal of Biology and Chemistry 36/4 (December 1, 2008): 313-318. https://izlik.org/JA69YW86LS.
JAMA
1.İspirli Y, Ayhan H. Proteic Feeder Effect on Glucose Oxidase Aggregates Formation. HJBC. 2008;36:313–318.
MLA
İspirli, Yasemin, and Hakan Ayhan. “Proteic Feeder Effect on Glucose Oxidase Aggregates Formation”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 4, Dec. 2008, pp. 313-8, https://izlik.org/JA69YW86LS.
Vancouver
1.Yasemin İspirli, Hakan Ayhan. Proteic Feeder Effect on Glucose Oxidase Aggregates Formation. HJBC [Internet]. 2008 Dec. 1;36(4):313-8. Available from: https://izlik.org/JA69YW86LS