Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts

Ö. Aylin Gürpınar; Handan Sevim; Mehmet Ali Onur; Fügen Dağlı; Zafer C. Çehreli

Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts

Abstract

The purpose of this study was to evaluate the cytotoxic and apoptotic potential of a fifthgeneration, total-etch etch & rinse adhesive system, Prime&Bond XP. For this purpose, cultured human pulp and gingival fibroblast cells were used. Cells were kept in 1% antibiotic and 10% fetal calf serum-containing DMEM/F12 at 37°C and 95% air-5% CO2 incubation atmosphere. The culture medium was replaced 2-3 times a week. The test materials were exposed to cells at 1:1000 and 1:4000 concentrations. Cell counting was performed on days 1, 3, 5 and 7; and untreated cells served as controls. Propidium iodide/acridine orange PI/AO staining was used to assess apoptosis of treated cells and of the control group at 7th day. The cells were visualized by fluorescence microscopy for the morphological determination of apoptosis. Compared with gingival fibroblasts, cultured pulp cells demonstrated more favorable response to the test solutions. However, at 1:1000 dilution, both cells showed significantly greater cell death and apoptosis.

Keywords

Pulp,Toxicity,Dentin adhesive,Apoptosis

References

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Ö. Aylin Gürpınar This is me

Handan Sevim This is me

Mehmet Ali Onur This is me

Fügen Dağlı This is me

Zafer C. Çehreli This is me

Publication Date

August 1, 2009

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2009 Volume: 37 Number: 3

IZ

https://izlik.org/JA92ZB53EH

Cite

RIS / Bibtex

APA

Gürpınar, Ö. A., Sevim, H., Onur, M. A., Dağlı, F., & Çehreli, Z. C. (2009). Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts. Hacettepe Journal of Biology and Chemistry, 37(3), 189-195. https://izlik.org/JA92ZB53EH

AMA

1.Gürpınar ÖA, Sevim H, Onur MA, Dağlı F, Çehreli ZC. Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts. HJBC. 2009;37(3):189-195. https://izlik.org/JA92ZB53EH

Chicago

Gürpınar, Ö. Aylin, Handan Sevim, Mehmet Ali Onur, Fügen Dağlı, and Zafer C. Çehreli. 2009. “Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts”. Hacettepe Journal of Biology and Chemistry 37 (3): 189-95. https://izlik.org/JA92ZB53EH.

EndNote

Gürpınar ÖA, Sevim H, Onur MA, Dağlı F, Çehreli ZC (August 1, 2009) Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts. Hacettepe Journal of Biology and Chemistry 37 3 189–195.

IEEE

[1]Ö. A. Gürpınar, H. Sevim, M. A. Onur, F. Dağlı, and Z. C. Çehreli, “Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts”, HJBC, vol. 37, no. 3, pp. 189–195, Aug. 2009, [Online]. Available: https://izlik.org/JA92ZB53EH

ISNAD

Gürpınar, Ö. Aylin - Sevim, Handan - Onur, Mehmet Ali - Dağlı, Fügen - Çehreli, Zafer C. “Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts”. Hacettepe Journal of Biology and Chemistry 37/3 (August 1, 2009): 189-195. https://izlik.org/JA92ZB53EH.

JAMA

1.Gürpınar ÖA, Sevim H, Onur MA, Dağlı F, Çehreli ZC. Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts. HJBC. 2009;37:189–195.

MLA

Gürpınar, Ö. Aylin, et al. “Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 3, Aug. 2009, pp. 189-95, https://izlik.org/JA92ZB53EH.

Vancouver

1.Ö. Aylin Gürpınar, Handan Sevim, Mehmet Ali Onur, Fügen Dağlı, Zafer C. Çehreli. Biocompatibility of a Fifth-Generation Adhesive System on Pulpal and Gingival Fibroblasts. HJBC [Internet]. 2009 Aug. 1;37(3):189-95. Available from: https://izlik.org/JA92ZB53EH