In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity

Şükrü Beydemir; Deniz Ekinci; Orhan Ateş

In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity

Abstract

Dexamethasone has successfully been used for treatment of some inflammatory diseases. Here, we evaluated the in vitro effects of dexamethasone on the activity of human serum paraoxonase hPON1 . PON1 enzyme was purified using simple chromatographic methods: DEAE-Sephadex anion exchanger and Sephadex G 200 gel filtration chromatography from human serum. hPON1 was purified approx. 225-fold with a final specific activity of 4867.3 U x mg-1 proteins and with a purity up to 39.8%. Dexamethasone dose-dependently decreased in vitro hPON1 activity. IC50 value for dexamethasone was determined as 1.106. Inhibition constant Ki was estimated as 3.284 ± 0.394 mM from Lineweaver-Burk graph. Inhibition type of dexamethasone was determined as noncompetitive. Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.

Keywords

Paraoxonase,Dexamethasone,Human serum,Inhibition,Enzyme purification

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Details

Primary Language

English

Subjects

-

Journal Section

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Authors

Şükrü Beydemir This is me

Deniz Ekinci This is me

Orhan Ateş This is me

Publication Date

August 1, 2009

Submission Date

-

Acceptance Date

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Published in Issue

Year 2009 Volume: 37 Number: 3

IZ

https://izlik.org/JA94FS79HN

Cite

RIS / Bibtex

APA

Beydemir, Ş., Ekinci, D., & Ateş, O. (2009). In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity. Hacettepe Journal of Biology and Chemistry, 37(3), 197-205. https://izlik.org/JA94FS79HN

AMA

1.Beydemir Ş, Ekinci D, Ateş O. In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity. HJBC. 2009;37(3):197-205. https://izlik.org/JA94FS79HN

Chicago

Beydemir, Şükrü, Deniz Ekinci, and Orhan Ateş. 2009. “In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity”. Hacettepe Journal of Biology and Chemistry 37 (3): 197-205. https://izlik.org/JA94FS79HN.

EndNote

Beydemir Ş, Ekinci D, Ateş O (August 1, 2009) In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity. Hacettepe Journal of Biology and Chemistry 37 3 197–205.

IEEE

[1]Ş. Beydemir, D. Ekinci, and O. Ateş, “In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity”, HJBC, vol. 37, no. 3, pp. 197–205, Aug. 2009, [Online]. Available: https://izlik.org/JA94FS79HN

ISNAD

Beydemir, Şükrü - Ekinci, Deniz - Ateş, Orhan. “In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity”. Hacettepe Journal of Biology and Chemistry 37/3 (August 1, 2009): 197-205. https://izlik.org/JA94FS79HN.

JAMA

1.Beydemir Ş, Ekinci D, Ateş O. In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity. HJBC. 2009;37:197–205.

MLA

Beydemir, Şükrü, et al. “In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 3, Aug. 2009, pp. 197-05, https://izlik.org/JA94FS79HN.

Vancouver

1.Şükrü Beydemir, Deniz Ekinci, Orhan Ateş. In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity. HJBC [Internet]. 2009 Aug. 1;37(3):197-205. Available from: https://izlik.org/JA94FS79HN