Antibacterial Agent Loaded Fungal Polymer for Use As A Wound Dressing

Abstract

This study is mainly concerned with the preparation and characterization of a novel wound dressing, in the form of three-dimensional microporous structure, from extracellular polysaccharide produced by Trametes versicolor, a white-rot basidiomycete. A model antibiotic i.e., ciprofloxacin was loaded into these wound dressings in the preparation procedure. The prepared wound dressings were investigated and evaluated in four main sections. In the first part, morphological evaluations were made by using scanning electron microscopy and the obtained images revealed that the wound dressings were having highly porous structure with interconnected pores. Antibacterial agent loaded-wound dressings were also investigated with swelling behavior, in vitro drug release and biodegradation studies. Obtained results showed that wound dressings from fungal polymer including antibacterial agent could provide a great potential in the treatment of dermal wounds as a new kind of wound dressing material.

Keywords

• Wound healing,wound dressing,Trametes versicolor,ciprofloxacin,natural polymer,exopolysaccharide,characterization

Yara Örtü Malzemesi Olarak Kullanılmak Üzere Antibakteriyel Ajan Yüklü Fungal Polimer

Abstract

B u çalışma beyaz çürükçül bir mantar suşu olan Trametes versicolor’ın ürettiği hücredışı polisakkaritten üçboyutlu mikrogözenekli yapıya sahip yeni bir yara örtü malzemesinin hazırlanması ve karakterizasyonunu kapsamaktadır. Hazırlama işlemi esnasında model bir antibiyotik siprofloksasin yara örtü malzemelerine yüklenmiştir. Elde edilen yara örtü malzemeleri 4 ana bölüm altında incelenmiştir. İlk bölümde taramalı elektron mikroskobu kullanılarak morfolojik değerlendirmeler yapılmış olup elde edilen görüntüler sonucunda yara örtü malzemelerinin birbirleri ile bağlantılı yüksek gözenekliliğe sahip olduğu gözlenmiştir. Antibakteriyel ajan yüklü yara örtü malzemeleri ayrıca şişme ve in vitro ilaç salımı çalışmaları, biyobozunurluk deneyleri yapılarak karakterize edilmiştir. Elde edilen sonuçlar mantar polimerden elde edilen antibakteriyel ajan yüklü yara örtü malzemelerinin yeni nesil yara örtü malzemesi olarak deri yaralarının tedavisinde iyi bir potansiyel olusturabileceğini göstermektedir

Keywords

• Yara iyileşmesi,yara örtü malzemesi,Trametes versicolor,siprofloksasin,dogal polimer,ekzopolisakkarit,karakterizasyon

References

1. E. Joseph, C.A. Hamori, S. Bergman, E. Roaf, N.F. Swann, G.W. Anastasi, A prospective, randomized trial of vacuum-assisted closure versus standard therapy of chronic non-healing wounds, Wounds. 12 (2000)60 .
2. K.S. Midwood, L.V. Williams, J.E. Schwarzbauer, Tissue repair and the dynamics of the extracellular matrix, Int. J. Biochem. Cell Biol., 36 (2004) 1031.
3. A.K. Tsirogianni, N.M. Moutsopoulos, H.M. Mout- sopoulos, Wound healing: immunological aspects, Injury. 37 (suppl 1) (2006) 5.
4. G.I. Broughton, J.E. Janis, C.E. Attinger, The basic science of wound healing, Plast Reconstr. Surg. 117 (7 suppl) (2006) 12.
5. M.B. Witte, A. Barbul, General principles of wound healing, Sur. Clin. North. 77 (1997) 509.
6. W.H. Eaglstein, V. Falanga, Chronic. wounds., Surg. Clin. North., 77 (1997) 689.
7. C. Weller, G.Sussman, Wound dressings update, J. Pharm. Prac. Res., 36 (2006) 318.
8. C.J. Doillon, Porous collagen sponge wound dressings: in vivo and in vitro studies, J. Biomater. Appl., 2 (1987) 562.

9. J.T. Shores, A. Gabriel, S. Gupta, Skin substitutes and alternatives: A review, Adv. Skin Wound Care, 20 (2007) 509.
10. T.S. Stashak, E. Farstvedt, A. Othic, Update on wound dressing: Indications and best use, Clin. Techniq. Equine Prac., 3 (2004) 148.
11. D.V. Kusum, S. Saisivam, G.R. Maria, P.U. Deepti, Design and evaluation of matrix diffusion controlled transdermal patches of verapamil hydrochloride, Drug Dev. Ind. Pharm., 29 (2003) 495.
12. C.A. Blanckmeister, D.H. Sussdorf, Macrophage activation by cross-linked dextran, J. Leuko. Biol., 37 (1985) 209.
13. I.W. Sutherland, Novel and established applications of microbial polysaccharides, TIBTECH, 16 (1998) 41.
14. B. Dülger, Ü. Arslan, Coriolus versicolor (L.ex Fr.) Quel. makrofungusunun antimikrobiyal aktivitesi, Tr. J. Biol., 23 (1999) 385.
15. N.H. Horowitz, M. Fking, G. Horn, Tyrosinase (Neurospora crassa), Methods in Enzymol., 17A (1970) 615.
16. K. Lerch, Monophenol Monooxygenase from Neurospora crassa, Method. Enzymology., 142 (1984) 165.
17. H. Çiçek, Beyaz-çürükçül fungus kültürlerinde tirosinaz enziminin sentezinin taranması ve optimizasyonu, MSc Thesis, Hacettepe University, Department of Biology, Ankara, 2000.
18. D. Candan, Characterization and optimization of biopolymer production by Trametes versicolor, PhD Thesis, Hacettepe University, Department of Biology, 2005.
19. E.B. Denkbaş, M. Odabaşı, Chitosan microspheres and sponges: Preparation and characterization, J. Appl Polym. Sci. 76 (2000) 1637.
20. E.B. Denkbaş, M. Seyyal, E.Pişkin, 5-Fluorouracil loaded chemoembolization, J. Microencapsul., 16 (1999) 741.
21. F. Cui, D. Cun, A. Tao, M. Yang, K. Shi, M. Zhao, Y. Guan, Preparation and characterization of melittin-loaded poly (dl-lactic acid) or poly (dl-lactic-co-glycolic acid) microspheres made by the double emulsion method
22. J. Controlled Release., 107 (2005) 310.
23. J.H. Ha, S.H. Kim, S.Y. Han, Y.K. Sung, Y.M. Lee, I.K. Kang, C.S. Cho, Albumin release from bioerodible hydrogels based on semi-interpenetrating polymer networks composed of poly(e-caprolactone) and poly(ethylene glycol) macromer, J. Controlled Release, 49 (1997) 253.
24. C.S. Cho, S.Y. Han, J.H. Ha, S.H. Kim, D.Y. Lim, Clonazepam release from bioerodible hydrogels based on semi-interpenetrating polymer networks composed of poly(o-caprolactone) and poly (ethylene glycol) macromer, Int. J. Pharm., 181 (1999) 235.
25. H. Peng, S.Zhou, T. Guo, Y. Li, X. Li, J. Wang, J. Weng, In vitro degradation and release profiles for electrospun polymeric fibers containing paracetanol, Colloid. Surf. B., 66 (2008) 206.