Activatory Effects of Oxytetracycline on Some α-Carbonic Anhydrases

Abstract

Three
-carbonic anhydrases, including human carbonic anhydrase I and II hCA I, II and sheep kidney carbonic anhydrase sCA were purified and kinetic interactions between the enzymes and oxytetracycline were investigated. The purification procedure was composed of preparation of homogenate or hemolysate and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. Oxytetracycline exhibited in vitro activatory effects on hCA I, II and sCA enzymes activity. Activity % values for this antibiotic were determined by plotting activity % vs. [A]

Keywords

• Carbonic anhydrase,Human,Sheep,Kidney,Activator,Oxytetracycline

Bazı Alfa-Karbonik Anhidrazlarda Oksitetrasiklinin Aktivatör Etkileri

Abstract

Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II hCA I, II ve koyun böbrek karbonik anhidraz sCA saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi. Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu. Oksitetrasiklin hCA I, II ve sCA enzim aktivitelerinde in vitro aktivatör etkisi gösterdi. Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi

Keywords

• Karbonik anhidraz,insan,koyun,böbrek,aktivatör,oksitetrasiklin

References

1. 1. http://en.wikipedia.org/wiki/Oxytetracycline
2. 2. R. Parui, K.K. Gambir, P.P. Mehrotra, Changes in carbonic anhydrase may be the initial step of altered metabolism in hypertension, Biochem. Int., 23(1991) 779.
3. 3. C.T. Supuran, A. Scozzafava, Carbonic anhydrase inhibitors and their therapeutic potential. Exp. Opin. Ther. Patents, 10 (2000) 575.
4. 4. D. Hewett-Emmett, Evolution and distribution of the carbonic anhydrase gene families. In: W.R. Chegwidden, N.D. Carter, Y.H. Edwards (Eds). The Carbonic Anhydrases. New Horizons. Basel: Birkhauser Verlag, (2000) pp 29.
5. 5. T. Stams, D.W. Christianson, X-ray crystallographic studies of mammalian carbonic anhydrase isozymes. In: W.R. Chegwidden, N.D. Carter, Y.H. Edwards, (Eds.), The Carbonic Anhydrases: New Horizons. Birkhauser Verlag, Boston (2000) pp. 159.
6. 6. F. Briganti, A. Scozzafava, C.T. Supuran, Novel carbonic anhydrase isozymes I, II, and IV activators incorporating sulfonylhistamino moieties. Bioorg. Med. Chem. Lett, 9 (1999) 2043.
7. 7. F. Briganti, V. Iaconi, S. Mangani, P. Orioli, A. Scozzafava, G. Vernaglione, C.T. Supuran, Carbonic anhydrase activators: the X-ray crystallographic characterization of the ternary complex of phenylalanine and azide with human isozyme II. lnorg. Chim. Acta, 275 (1998) 295.
8. 8. B.W. Clare, C.T. Supuran, Carbonic anhydrase activators. 3: structure-activity correlations for a series of isozyme II activators. J. Pharm. Sci, 83 (1994) 768.

9. 9. C.T. Supuran, A.T. Balaban, P. Cabildo, R.M. Claramunt, J.L. Lavandera, J. Elguero, Carbonic anhydrase activators. VII. Isozyme II activation by bisazolylmethanes, -ethanes and related azoles. Biol. Pharm. Bull, 16 (1993) 1236.
10. 10. E. Bayram, M. Senturk, O.I. Kufrevioglu, C.T. Supuran, In vitro inhibition of salicylic acid derivatives on human cytosolic carbonic anhydrase isozymes I and II, Bioorg. Med. Chem, 16 (2008) 9101.
11. 11. S.B. Ceyhun, M. Senturk, O. Erdogan, O.I. Kufrevioglu, In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills. Pesticide Biochem. Physiol, 97 (2010) 177.
12. 12. K.M. Wilbur, N.G. Anderson, Electrometric and colorimetric determination of carbonic anhydrase J. Biol. Chem, 176 (1948) 147.
13. 13. J.A. Verpoorte, S. Mehta, J.T. Edsall, Esterase activities of human carbonic anhydrase. J. Biol. Chem, 242 (1967) 4221.
14. 14. H. Lineweaver, D. Burk, The determination of enzyme dissocation constants. J. Am. Chem. Soc, 57 (1934) 685.
15. 15. M.M. Bradford, A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem, 72 (1976) 248.
16. 16. D.K. Laemmli, Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227 (1970) 680.
17. 17. D. Ekinci, H. Cavdar, O. Talaz, M. Senturk, C.T. Supuran, NO-releasing esters show carbonic anhydrase inhibitory action against human isoforms I and II. Bioorg. Med. Chem, 18 (2010) 3559.
18. 18. Ekinci, D., Beydemir, S., Kufrevioglu, O.I., In vitro inhibitory effects of some heavy metals on human erythrocyte carbonic anhydrases. J. Enzyme Inhib. Med. Chem, 22 (2007) 745.
19. 19. S. Beydemir, M. Ciftci, I. Ozmen, M.E. Buyukokuroglu, H. Ozdemir, O.I. Kufrevioglu, Effects of some medical drugs on enzyme activities of carbonic anhydrase from human erythrocytes in vitro and from rat erythrocytes in vivo Pharmacol. Research, 42 (2000) 187.