In vitro antimicrobial and antioxidant activities and GC/MS analysis of the essential oils of Rumex crispus and Rumex cristatus

Abstract

In this study, was investigated in vitro antimicrobial and antioxidant activities and GC/MS analysis of the essential oil of Rumex crispus and Rumex cristatus collected from Corum province and around. Total antio- xidant status of essential oils prepared with ethanol and pure water were measured spectrophotometrically. Staphylococcus aureus ATCC 25923, Eschrechia coli ATCC 25922, Enterecoccus faecalis ATCC 29212 and Can- dida albicans ATCC 10231 were used to the antimicrobial studies. The antimicrobial properties of samples were determinate with the disk diffusion and agar-well method. The total antioxidant status in the essential oils of all Rumex cristatus contained high levels was determinate according to Rumex crispus. The developing of test microorganisms of the essential oils of all Rumex crispus and Rumex cristatus extracts have been observed to inhibit varying degrees on the antimicrobial activity test. Furthermore, extracts obtained with ethanol is more effective according to chloroform extracts and more efficient results which have been identified. Enterecoc- cus faecalis ATCC 29212, Escherichia coli ATCC 25922 Staphylococcus aureus ATCC 25923 and fungal culture of Candida albicans ATCC 10231. The diphenic acid derivatives were found the content of two Rumex sp. essential oils with GC/MS analysis.

Keywords

• Rumex crispus,Rumex cristatus,essential oils,antioxidant activity,antimicrobial activity,GC-MS

Rumex crispus ve Rumex cristatus’un uçucu yağlarının in vitro antimikrobiyal ve antioksidan aktiviteleri ile GC/MS analizi

Abstract

B u çalışmada Çorum ili ve çevresinden toplanan Rumex crispus ve Rumex cristatus’un uçucu yağlarının antimikrobiyal ve antioksidan aktiviteleri ile GC/MS analizleri araştırıldı. Etanol ve saf su ile hazırlanan örneklerin uçucu yağlarının toplam antioksidan statüsü spektrofotometrik olarak ölçüldü. Antimikrobiyal çalışmalarda Staphylococcus aureus ATCC 25923, Eschrechia coli ATCC 25922, Enterecoccus faecalis ATCC 29212 ve Candida albicans ATCC 10231 türleri kullanıldı. Örneklerin antimikrobiyal özellikleri disk ve agar-kuyu yöntemi ile belirlendi. Tüm Rumex cristatus uçucu yağlarının total antioksidan miktarı, Rumex crispus’a göre daha yüksek bulundu. Antimikrobiyal aktivite testlerinde Rumex crispus ve Rumex cristatus uçucu yağlarının test mikroorganizmalarının gelişimini farklı derecelerde inhibe ettiği gözlendi. Etanol ile elde edilen uçucu yağların kloroforma göre daha etkin olduğu tanımlandı

Keywords

• Rumex crispus,Rumex cristatus,uçucu yağ,antioksidan aktivite,antimikrobiyal aktivite,GC-MS

References

1. 1. A.A. El-Bakry, H.A. Mostafa, A. Alam Eman, Antibacterial and antioxidant activities of seedlings of Rumex vesicarius L. (Polygonaceae), J. Med. Plan. Res., 7 (2013) 1754
2. 2. P.H. Davis, R.R. Mill, K. Tan, Flora of Turkey and the East Aegean Islands. Edinburgh University Press, Edinburgh, 2, 1988.
3. 3. T. Baytop, Therapy with Medicinal Plants in Turkey, Past and Present. Second Edition, İstanbul: Nobel Tıp Kitabevi, 1999.
4. 4. A.A. Boligon, A. C. Feltrin, M. L. Athayde, Determination of chemical composition, antioxidant and antimicrobial properties of Guzuma ulmifolia essential oil, Am. J. Essen. Oil. Nat. Pro., 1 (2013) 23.
5. 5. A. Adıgüzel, H. Ozer, M. Sokmen,M. Gulluce, A. Sokmen, H. Kılıc, F. Sahin, O. Barıs, Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria, Polish J. Microbiol., 58 ( 2009) 69.
6. 6. M. Chua, Y. Tung, S. Chang, Antioxidant activities of ethanolic extracts from the twigs of Cinnamomum osmophloeum, Bioresour. Technol., 99 (2008) 1918.
7. 7. G.A. Avcı, E. Avcı, D.A. Kose, H. Akcal, Investigation of applicability instead of chemical disinfectants of Euphorbia orientalis L. extracts, Hacettepe J. Biol. Chem., 41 (2013) 151.
8. 8. L. Barros, L. Cabrita, M.V. Boas, A.M. Carvalho, I.C.F.R. Ferreira, Chemical, biochemical and electrochemical assays to evaluate phytochemicals and antioxidant activity of wild plants, Food Chem., 127 (2011) 1600.

9. 9. I. Dahech, W. Farahc, M. Triguib, A. B. Hssouna, H. Belghithc, K. S. Belghitha, F. B. Abdallah, Antioxidant and antimicrobial activities of Lycium shawii fruits extract, Inter. J. Biol. Macro., 60 (2013) 328.
10. 10. J. Liu, L. Jia, J. Kan, C. Jin, In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus), Food Chem. Toxicol., 51 (2013) 310.
11. 11. T. Essawi, M. Srour, Screening of some Palestinian medicinal plants for antibacterial activity. J. Ethnophar.,, 70 (2000) 343.
12. 12. B. Tepe, D. Daferera, A. Sokmen, M. Sokmen, M. Polissiou, Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae ), Food Chem., 90 (2005) 333.
13. 13. C. Sarikurkcu, B. Tepe, D.K. Semiz, M.H. Solak, Evaluation of metal concentration and antioxidant activity of three edible mushrooms from Mugla, Turkey, Food Chem. Toxicol., 48 (2010) 1230.
14. 14. I. Palacios, M. Lozano, C. Moro, M. D’Arrigo, M.A. Rostagno, J.A. Martínez, A. García-Lafuente, E. Guillamón, A. Villares, Antioxidant properties of phenolic compounds occurring in edible mushrooms, Food Chem., 128 (2011) 674.
15. 15. Y.T. Liu, J. Sun, Z.Y. Luo, S.Q. Rao, Y.J. Su, R.R. Xu, Y.J. Yang, Chemical composition of five wild edible mushrooms collected from Southwest China and their antihyperglycemic and antioxidant activity, Food Chem. Toxicol., 50 (2012) 1238.
16. 16. F.S. Reis, A. Martins, L. Barros, I.C.F.R. Ferreira, Antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms: a comparative study between in vivo and in vitro samples Food Chem. Toxicol., 50 (2012) 1201.
17. 17. A. Kalemba, A. Kunicka, Antibacterial and antifungal properties of essential oils, Curr. Med. Chem., 10 (2003) 813.
18. 18. O. Erel, A novel automated method to measure total antioxidant response against potent free radical reactions, Clin. Biochem., 37 (2004) 112.
19. 19. I. Coruh, A.A. Gormez, S. Ercisli,M. Sengul, Total Phenolic Content, Antioxidant, and Antibacterial Activity of Rumex crispus Grown Wild in Turkey, Pharma. Biol., 46 (2008) 634.
20. 20. Z. Maksimovic, N. Kovacevic, B. Lakušic, T. Cebovic, Antioxidant Activity of Yellow Dock (Rumex crispus L., Polygonaceae) Fruit Extract, Phytother. Res., 25 (2011) 101.
21. 21. A. Yildirim, A. Mavi, A. Kara, Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts, J. Agric. Food Chem., 49 (2001) 4083.
22. 22. A.A. Elzaawely, T.D. Xuan, S. Tawata, Antioxidant and Antibacterial Activities of Rumex japonicus HOUTT. Aerial Parts, Biol. Pharm. Bull., 28 (2005) 2225.
23. 23. A.A. Elzaawely, S. Tawata, Antioxidant Capacity and Phenolic Content of Rumex dentatus L. Grown in Egypt, J. Crop Sci. Biotech.,15 (2012) 59.