Human IgG Adsorption by Spherical Albumin Biosorbents in SBF Media

Abstract

The objective of this study was to investigate the removal efficiency of immunoglobulin G IgG by albumin bi- osorbents in batch wise in four different simulated body fluid SBF solutions at room temperature. Albumin biosorbents were prepared as microsphere form by emulsion polymerization method. 0.1 g/ml albumin concentration, 1000 rpm stirring rate, 1% glutaraldehyde GA concentration 30 minutes crosslinking time were determined as optimal conditions in our previous study. The prepared biosorbents were then studied for IgG removal efficiency in four simulated body fluid SBF solutions [N Normal -SBF, G glucose -SBF, H hepes -SBF, T tris -SBF] at room temperature. IgG capturing capacity was obtained as 320 mg IgG/g with albümin biosor- bent 94,6% in N-SBF. It was observed that there was no leakage at washing with water. It may be concluded that prepared albumin biosorbents are sufficient in terms of efficiency of IgG removal. It was seem that this method is easy to perform and is useful as a first step in the detection of diagnostic markers in body fluids by applying proteomics technologies. We believe that albumin biosorbents offered the promising approach with good removal specificity and efficiency of IgG.

Keywords

• Biosorbent,Albumin,IgG,SBF

Küresel Albumin Biyosorbentlerle SBF ortamlarında İnsan IgG Adsorpsiyonu

Abstract

B u çalışmanın amacı albümin biyosorbentlerin, kesikli sistemde dört farklı yapay vücut sıvısı ortamında İnsan immunglobulin G HIgG uzaklaştırma kapasitesinin araştırılmasıdır. Albumin biyosorbentler, emülsiyon polimerizasyon yöntemi ile mikroküre formunda hazırlandı. Denysel parametreler 0,1 g/ml albumin derişimi, 1000 rpm karıştırma hızı, %1 glutaraldehid derişimi ve 30 dakika çapraz bağlanma süresi optimum şartlar olarak daha önceki çalışmamızda belirlenmiştir. Daha sonra, hazırlanan biyosorbentlerle, oda sıcaklığında dört faklı yapay vücut sıvısı SBF çözeltisi [N Normal -SBF, G glukoz -SBF, H hepes -SBF, T tris -SBF] ortamında, HIgG uzaklaştırma çalışmaları yapılmıştır. Albumin biyosorbentlerle N-SBF ortamında HIgG yakalama kapasitesinin 320 mg HIgG/g %94,6 olduğu tespit edilmiştir. Saf su ile yıkama işlemlerinde kaçak olmadığı gözlemiştir. Hazırlanan albumin biyosorbentlerin HIgG uzaklaştırma verimliliği açısından yeterli olduğu sonucuna varılmıştır. Bu yöntemin, proteomik teknolojileri uygulayarak vücut sıvılarında teşhis belirteçlerinin saptanmasında, ilk adım olarak gerçekleştirilmesi, kolay ve yararlı olduğu görülmektedir. Albumin biyosorbentler, iyi bir uzaklaştırma seçiciliği ve etkinliği ile umut verici yaklaşım sunmaktadır

Keywords

• Biosorbent,Albumin,IgG,SBF

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