Determination of Volatile Composition of Ozone Treated Arils From Longterm-Stored Whole Pomegranate Fruits Using HS- SPME GC/MS Technique

Abstract

Long-term stored whole ‘Hicaznar’ pomegranate fruit arils were exposured to three different concentrations 0 mg/h/m3, 5.2 mg/h/m3, 10.4 mg/h/m3 of ozon treatment and then minimally processed pomegranate arils stored at +5oC and 85-90% RH during 12 days. Effects of ozone treatment to ‘Hicaznar’ pomegranate arils aroma volatile compositions was were analyzed by using HS-SPME/GC/MS Headspace Solid Phase Micro Extraction Gas Chromatography Mass Spectrometry techniques. The results of research showed that aldehydes, terpenes, alcohols, acids and ester were main aroma volatiles in ‘Hicaznar’ pomegranate fruits arils at the end of storage. And terpens which are D- limonene, α-pinene, trans-a-bergamotene, 1.3.6.10-dodecatriene and caryophyllene percentage were higher in control than ozone treated fruits. However, the volatiles such as acetaldehyde, acetic acid and ethyl acetate were lower percentage in ozone treated fruits compared control one at the end storage.

Keywords

• Pomegranate,volatiles,HS-SPME GC/MS,headspace

Uzun Süre Depolanmış Nar Meyvelerinde Ozon Uygulamasının Nar Taneleri Uçucu Aroma Bileşiklerine Etkisinin HS-SPME GC/ MS Tekniği ile Belirlenmesi

Abstract

Bütün olarak uzun süreli depolanan ‘Hicaznar’ nar meyvelerinden alınan tanelere, 0 mg/saat/m3 5.2 mg /saat/m3, 10.4 mg/h/m3 olacak şekilde ozon uygulamasına maruz bırakıldıktan sonra 12 gün boyunca +5oC sıcaklık ve %85-90 oransal nem koşullarında muhafaza edilmiştir. Ozon uygulamasının nar meyvesi uçucu aroma bileşimine olan etkileri HS-SPME/GC/MS Headspace Solid Phase Micro Extraction Gas Chromatography Mass Spectrometry teknikleri kullanılarak belirlenmiştir. Çalışmada kullanılan ‘Hicaznar’ nar meyve suyu örneklerinde sırası ile aldehitler, terpenler, alkoller, asitler ve esterlerin temel uçucu aroma bileşikleri olduğu tespit edilmiştir. Terpen grubundan olan D- limonen, α-pinen, trans-a- bergamoten, 1.3.6.10-dodekatrien ve karyophilen bileşiklerinin kontrol grubu meyvelerinde ozon uygulanan meyvelerden daha yüksek olduğu tespit edilmiştir. Fakat Ozon ile muamele edilmiş meyve suyu bileşiminde asetaldehit, asetik asit ve etil asetat gibi uçucu aroma bileşiklerinin miktarının kontrol grubu meyvelerinde ozon uygulanan meyvelerden daha fazla olduğu belirlenmiştir

References

1. Z. Ayhan, O. Eştürk, Overall quality and shelf life of minimally processed and Modified Atmosphere Packaged “Ready-to-eat” pomeranate arils, J. Food. Sci., 74 (2009) 399-405.
2. V. Lopez-Rubira, A. Conesa, A. Allende, F. Artes, Shelf life and overall quality of minimally processed pomegranate arils modified atmosphere packaged and treated with UV-C, Postharvest Biol. Technol., 37 (2005) 174-185.
3. A.T. Oz, Z. Ulukanli, Application of edible starch-based coating including glycerol plus oleum nigella on arils from long-stored whole pomegranate fruits, J. Food. Proces. Preserv., 36 (2012) 81–95.
4. F.A. Miller C. L. M. Silva, T. R. S. Branda˜o, A Review on Ozone-Based Treatments for Fruit and Vegetables Preservation, Food. Eng. Rev., 5 (2013) 77–106.
5. X. Zhang, Z. Zhang, L.Wang, Z. Zhang, J. LI, C. Zhao, Impact of ozone on quality of strawberry during cold storage, Front. Agric. China, 5 (2011) 356–360.
6. H. Ikeura, F. Kobayashi, M. Tamaki. Ozone microbubble treatment at various water temperatures fort he removal of residual pesticides with negligible effects on the physical properties of lettuce and cherry tomatoes. Journal of Food. Sci., 78 (2013) 350-355.
7. E. Savaş, H. Tavşanlı, İ. Gökgözoğlu, Ozone application in food industry, Türk Tarım- Gıda Bilim ve Teknol, 2 (2014) 122-127.
8. H. Karaca, S. Velioglu, Ozone Application in fruit and vegetable processing, Food Review Int., 23 (2007) 91- 106.

9. P. Melgarejo, A. Calin-Sanchez, L. Vazquez-Araujo, F. Hernandez, J.J. Martinez, P. Legua, A. Carbonell- Barrachina, Volatile composition of pomegranates from 9 spanish cultivars using heaspace solid phase microextraction, J. Food Sci., 76 (2011) 114-120.
10. E. Kafkas, S. Kafkas, M. Koch-Dean, W. Schwab, O. Larkov, N. Lavid, E. Bar, U. Ravid, E. Lewinsohn. Comparison of methodologies for the identification of aroma compounds in strawberry, Turk. J. Agric. Forest., 29 (2005) 383-390.
11. C.F. Forney, J. Song, P.D. Hildebrand, L. Fan, K.B. McRae. Interactive effects of ozone and 1-methylcyclopropene on decay resistance and quality of stored carrots, Postharvest Biol. Tech., 45 (2007) 341-348.
12. M.E. Saltveit, Effect of alcohols and their interaction with ethylene on the ripening of epidermal pericarp discs of tomato fruit, Plant Physiol., 90 (1988) 167–174.
13. P.M.A. Toivonen, Non-ethylene, non-respiratory volatiles in harvested fruits and vegetables: their occurrence, biological activity and control. Postharvest Biol. Technol., 12 (1997) 109–125.
14. T.W. Kimmerer, T.T. Kozlowski, Ethylene, ethane, acetaldehyde and ethanol production by plants under stress, Plant Physiol., 69 (1982) 840–847.
15. M. Larsen, C.B. Watkins, Firmness and aroma composition of strawberries following short-term high-carbon dioxide treatments, HortScience, 30 (1995) 303-305.