Botanic Origin, Various Physicochemical and Antioxidant Properties of Honey Samples From Giresun, Turkey

Abstract

The aim of the present study is to evaluate the botanic origin, various pysicochemical parameters pH, mo- isture, hydroxymethylfurfural HMF , fructose/glucose ratio and antioxidant properties of the four honey samples colected from Giresun-Black Sea Region of Turkey. According to the mellisopalynological analyis, two of the investigated honey samples were determined as Castanea sativa honey and the other two samples were determined as multifloral honeys.The pH value of honey samples were found between 3.11 and 4.44. The moisture ot the investigted samples were varied between 11.59-14.13%. The fructose contents of the samples were found between 36.58 and 43.42 g/100 g and glucose values as 24.84-33.55 g/100 g. HMF values were 1.6±1.0 mg/kg for Castanea sativa honeys and 0.65±0.66 mg/kg for multifloral honeys. Total phenol contents were high for Castanea sativa honeys compare to two multifloral honeys but total flavonoid content is found as highest in sample 3 multifloral honey .

Keywords

• Antioxidant properties,botanic origin,pysicochemical parameters,total phenolic content,total flavanoid content

Giresun-Türkiye’den Toplanan Bal Örneklerinin Bitkisel Kökeni, Fizikokimyasal ve Antioksidan Özellikleri

Abstract

B u çalışmanın amacı Türkiye’nin Giresun-Karadeniz Bölgesi’nden toplanan dört bal örneğinin bitkisel kökeni, fizikokimyasal parametreleri pH, nem, hidroksimetilfurfural, früktoz/glukoz oranı ve antioksidan özelliklerinin değerlendirilmesidir. Palinolojik analizlere göre, incelenen iki örnek Castanea sativa balı diğer ikisi de multifloral bal olarak tanımlanmıştır. Bal örneklerinin pH değerleri 3.11 ve 4.4 aralığında bulunmuştur. Nem değerleri ise %11.59-14.13 aralığındadır. Örneklerin früktoz içerikleri 36.58 ve 43.42 g/100 g aralığında ve glukoz içerikleri ise 24.84-33.55 g/100 g aralığında bulunmuştur. Castanea sativa ballarının HMF değerleri 1.6±1.0 mg/kg, multifloral balların ise 0.65±0.66 mg/kg olarak bulunmuştur. Castanea sativa ballarının toplam fenol miktarları multifloral ballara oranla yüksek çıkmıştır. Toplam flavonoid miktarları ise 3 numaralı örnekte en yüksek oranda bulunmuştur

Keywords

• Antioksidan özellik,botanik köken,fizikokimyasal parametre,toplam fenolik içerik,toplam flavanoid içerik

References

1. 1. E. Anklam, A review of the analytical methods to determine the geographical and botanical origin of honey, Food Chem., 63 (1998) 549-562.
2. 2. J.M. Alvarez-Suarez, S. Tulipani, S. Romandini, E. Bertoli, M. Battino, Contribution of honey in nutrition and human health: a review, Mediterranean Journal of Nutrition and Metabolism, 3 (2010), 15-23.
3. 3. A. Islam, I. Khalil, N. Islam, M. Moniruzzaman, A. Mottalib, S.A. Sulaiman, S.H. Gan, Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year, BMC Complem. M. Altern., 12 (2012) 177.
4. 4. D. Arreaz-Roman, A.M. Gomez-Caravaca, M. GomezRomero, A. Segura-Carretero,. A. FernandezGutierrez, Identification of phenolic compounds in rosemary honey using solid-phase extraction by capillary electrophoresis–electrospray ionizationmass spectrometry, J. Pharm. Biomed. Anal., 41 (2006) 1648–1656.
5. 5. A. Perna, A. Simonetti, I. Intaglietta, A. Sofo, E. Gambacorta, Metal content of southern Italy honey of different botanical origins and its correlation with polyphenol content and antioxidant activity, Int. J. Food Sci. Tech., 47 (2012) 1909–1917.
6. 6. EU DIRECTIVE /110/2001 of 02/12/2001 (L 10/47).
7. 7. J. Louveaux, A. Maurizio, G. Vorwohl, 1978. Methods of melissopalynology, Bee World, 59 (1978) 139-153.
8. 8. N. Moar, Pollen analysis of New Zealand honey, New Zea.l J. Agr. Res., 28 (1986) 39-70.

9. 9. A. Maurizio, Microscopy of honey, In Crane, E (Ed). Honey, a comprehensive survey (2nd edition). Heinemann; London, UK., (1975) 240–257 pp.
10. 10. IHC, 2002, International Honey Commission–IHC. (2002) Swiss Bee Research Centre, FAM, Liebefeld, CH-3003 Bern, Switzerland.
11. 11. Y.C. Chung, C.T. Chang, W.W. Chao, C.F. Lin, S.T. Chou, Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR–NK1, J. Agric. Food Chem., 50 (2002) 2454–2458.
12. 12. K. Slinkard, V.L. Singleton, Total phenol analyses: automation and comparison with manual methods, Am. J. Enol. Viticult., 28 (1977) 49–55.
13. 13. M.S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 181 (1958) 1199–1200.
14. 14. R.J. Ruch, S.J. Cheng, J.E. Klaunig, Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea, Carcinogenesis, 10 (1989), 1003–1008.
15. 15. A. Giorgi, M. Madeo, J.Baumgartner, G.C. Lozzia, The relationships between phenolic content, pollen diversity, physicochemical information and radical scavenging activity in honey, Molecules, 16 (2011) 336–347.
16. 16. J. Atanassova, L. Yurukova, M. Lazarova, Pollen and inorganic characteristics of Bulgarian unifloral honeys, J. Czech. Sci. Food, 30 (2012) 520–526.
17. 17. B. Fallico, M. Zappalà, E. Arena, A. Verzera, Effects of conditioning on HMF content in unifloral honeys, Food Chem., 85 (2004) 305–313.
18. 18. C. Ünal, Ö. Küplülü, Chemical quality of strained honey consumed in Ankara, Ankara Üniv. Vet. Fak. Derg, 53 (2006) 1-4.
19. 19. E. Derebasi, G. Bulut, M. Col, F. Guney, N. Yasar, O. Erturk, Physicochemical and residue analysis of honey from Black Sea Region of Turkey, Fresen Environ. Bull., 23 (2014) 10-17.
20. 20. A. Terrab, M.J. Díez, F.J. Heredia, Palynological, physico-chemical and colour characterization of Moroccan honeys: III. Other unifloral honey types, Int. J. Food Sci. Tech., 38 (2003) 395–402.
21. 21. Jr.J.W. White, Physical characteristics of honey. In Crane, E (Ed), In: Honey, a Comprehensive Survey., Heinemann; London, UK., (1975) 207-293.
22. 22. F. Buba, A. Gidado, A. Shugaba, Analysis of biochemical composition of honey samples from North-East Nigeria, Biochem. Anal. Biochem., 2 (2013) 139.
23. 23. M.C. Ciappini, M.B. Gatti, M.V. Di Vito, S. Gattuso, M. Gattuso, Characterization of different floral origins honey samples from Santa Fe (Argentine) by palynological, Physicochemical and Sensory data, Apiacta, 43 (2008) 25–36.
24. 24. Z. Can, O. Yildiz, H. Şahin, E.A. Turumtay, S. Silici, S. Kolayli, An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles, Food Chem., 180 (2015) 133–41.
25. 25. P. Andrade, F.Ferreres, M.T. Amaral, Analyses of honey phenolic acids by HPLC, its application to honey botanical characterization, J. Liq. R.T. Chromatogr., 20 (1997) 2281–2288.
26. 26. E. Mendes, E.B. Proenca, I.M.P.L.V.O. Ferreira, M.A. Ferreira, Quality evaluation of Portuguese honey, Carbohyd. Polym., 37 (1998) 219–223.
27. 27. Y. Amir, A. Yesli, M.Bengana, R. Sadoudi, T. Amrouche, Physico-chemical and microbiological assessment of honey from Algeria, EJEAFChe., 9 (2010) 1485–1494.
28. 28. Priyadharshini R.D.; Infee sherley, K. Review on production of 5-Hydroxymethylfurfural from carbohydrate rich biomass, International Journal of ChemTech Research, 8 (2015) 704-710.
29. 29. M. Zappalà, B. Fallico, E. Arena, A. Verzera, Methods for the determination of HMF in honey: a comparison, Food Control, 16 (2005) 273–277.
30. 30. M. Moniruzzaman, S.A. Sulaiman, M.I. Khalil, S.H. Gan, Evaluation of physicochemical and antioxidant properties of sourwood and other Malaysian honeys: a comparison with manuka honey, Chem. J Cent., 7 (2013) 1-12.
31. 31. P.K. Bath, N. Singh, A comparison between Helianthus annuus & Eucalyptus lanceolatus honey, Food Chem. 67 (1999) 389–397.
32. 32. A. Cherchi, M. Porcu, L. Spariedda, C.I.G Tuberoso, Influence of ageing on the quality of honey [Sardinia], Ind. Conserve, 72 (1997) 266–271.
33. 33. T.M. Rababah, M. Al-Omoush, S. Brewer, M. Alhamad, W. Yang, M. Alrababah, A.L.M. Al-Ghzawi, A.M. U’datt, K. Ereifej, F. Alsheyab, R. Esoh, A. Almajwal, Total phenol, antioxidant activity, flavonoids, anthocyanins and color of honey as affected by floral origin found in the arid and semiarid mediterranean areas, J. Food Process. Pres., 38 (2014) 1119–1128.
34. 34. S. Islam, J.S. Jothi, M. Islam, A. Zubair, Antioxidant and physico-chemical properties of Litchi honey procured from gazipur and tangail district, bangladesh, Journal of Entomology and Zoology Studies, 2 (2014) 207-211.
35. 35. J. Lachman, M. Orsák, A. Hejtmánková, E. Kováová, Evaluation of antioxidant activity and total phenolics of selected Czech honeys. Lebensm, Wiss. Technol. 43 (2010) 52-58.
36. 36. Ö. Erturk, H. Şahin, S. Kolayli, M. Çol Ayvaz, Antioxidant and antimicrobial activity of east black sea region honeys, Turk J. Biochem. 39 (2014) 99–106
37. 37. A.O. Sarikaya, E. Ulusoy, N. Öztürk, M. Tunçel, S. Kolayli, Antioxidant activity and phenolic acid constituents of chestnut (Castania sativa Mill.) honey and propolis, J. Food Biochem., 33 (2009) 470-481