Poly hydroxyethyl methacrylate -co-N-methacryloyl- L - histidine methyl ester Based Cryogel for the Removal of Fe3+ from Human Plasma effected with Beta Thalassemia

Abstract

In this study, Fe3+ adsorption was carried by using supermacroporous poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel. Poly HEMA-MAH cryogel was characterized by swelling tests and scanning electron microscopy. The influence of flow rate, pH, initial Fe3+ concentration, and Fe3+ adsorption from human plasma effected with beta thalassemia on the adsorption efficiency of the cryogel was investigated. The equilibrium swelling degree of poly HEMA-MAH cryogel was found to be 7.54 g H2O/g cryogel. Fe3+ adsorption capacity of poly HEMA-MAH cryogel from aqueous solution was estimated as 1.79 mg/g cryogel, while lower adsorption capacity was observed in human plasma 1.71 mg/g . It was also observed that Fe3+ could be repeatedly adsorbed and desorbed using poly HEMA-MAH cryogel without signi- ficant loss in its adsorption capacity.

Keywords

• Fe3+ removal,Beta Thalassemia,Affinity chromatography,cryogel,poly HEMA-MAH

Poli hidroksietil metakrilat-N-metakrilo-L-Histidin metil ester Temelli Kriyogel ile Beta talasemili İnsan Plazmasından Fe+3’ün Uzaklaştırması

Abstract

B u çalışmada, Fe3+ adsorpsiyonu, süpermakrogözenekli poli 2-hidroksietil metakrilat-N-metakrilo- I -histidin metil ester [poli HEMA-MAH ] kriyojeli kullanılarak incelenmiştir. Poli HEMA-MAH kriyojeli elektron mikroskop taraması ve şişme testleriyle karakterize edilmiştir. Akış hızı, pH, Fe3+’ün başlangıç derişiminin Fe3 adsorpsiyonuna etkileri incelenmiştir. Poli HEMA-MAH temelli kriyojelin denge şişme derecesi 7.54 g H0/g kriyojel olarak bulunmuştur. Poli HEMA-MAH temelli kriyojelin Fe3+ adsorpsiyon kapasitesi 1.71 mg/g bulunmuştur. Önemli miktarda adsorpsiyon kapasitesi kaybı olmadan Fe3+ iyonlarının P HEMA-MAH kriyojel ile adsorbe ve desorbe edildiği saptanmıştır

Keywords

• Fe3+ uzaklaştırılması,Beta Talasemi,Afinite Kromatografisi,Kriyojel,p HEMA-MAH

References

1. D. Purves, Trace Element Contamination of the Environment, Elsevier Science Publishers BV, Amsterdam, 1985.
2. B. Tang, L. Zhang, J. Zhang, Z. Z. Chen, Y.Wang, Spectrochim. Acta, 60 (2004) 2425.
3. M.C. Rouan, F. Marfil, P. Mangoni, R. Sechaud, H.Humbert, G. Maurer,. J. Chromatogr., B, 755 (2001) 203.
4. S. Ozkara, R. Say, C. Andac, Ind. Eng. Chem. Res., 47 (2008) 7849-7856
5. A. Denizli, B. Salih, E. Piskin, J. Biomater. Sci. Polym. Ed., 9 (1998) 175-187. (2001) 186-194.
6. M. Andac, E. Ozyapi, S. Senel, R. Say, A. Denizli, Ind. Eng.Chem. Res., 45 (2006) 1780-1786.
7. G. Baydemir, M. Andac, N. Bereli, R. Say, A. Denizli, Ind. Eng. Chem. Res., 46 (2007) 2843-2852.
8. M. McCoy, K. Kalghatgi, F.E. Regnier, N. Afeyan, J. Chromatogr. A, 743 (1996) 221–229.

9. E.B. Altıntas, A. Denizli, J. Chromatogr. B, 832 (2006) 216–223.
10. L. Uzun, R. Say, A. Denizli, React. Funct. Polym., 64 (2005) 93–102.
11. K. Thommes, M.R. Kula, Biotechnol. Prog., 11 (1995) 357–367.
12. T.B. Tennikova, B.J. Reusch, J. Chromatogr. A, 1065 (2005) 13–17.
13. N. Bereli, M. Andacβ , G. Baydemir, R. Say, I.Y. Galaev, A. Denizli, J. Chromatogr. A, 1190 (2008) 18–26.
14. F. Yılmaz, N. Bereli, H. Yavuz, A. Denizli, Biochem. Eng. J., 43 (2009) 272–279.
15. H. Alkan, N. Bereli, Z. Baysal, A. Denizli, Biochem. Eng. J., 45 (2009) 201–208.
16. G. Baydemir, N. Bereli, M. Andacβ , R. Say, I.Y. Galaev, A. Denizli, React. Funct. Polym., 69 (2009) 36–42.
17. G. Baydemir, N. Bereli, M. Andacβ , R. Say, I.Y. Galaev, A. Denizli, Colloid Surf. B, 68 (2009) 33–38.
18. L. Uzun, H. Yavuz, R. Say, A. Ersoz, A. Denizli, Ind. Eng. Chem. Res., 43 (2004) 6507–6513.
19. A. Cavus, Z. Baysal, H. Alkan, Colloids and Surfaces B: Biointerfaces, 107 ( 2013) 84–89
20. V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid, B. Mattiasson, Trends Biotechnol., 21 (2003) 445–451.
21. P. Arvidsson, F.M. Plieva, V.I. Lozinsky, I.Y. Galaev, B. Mattiasson, J. Chromatogr., A 986 (2003) 275–290.
22. V.I. Lozinsky, F.M. Plieva, I.Y. Galaev, B. Mattiasson, Bioseparation 10 (2002) 163–188.
23. W. Noppe, F.M. Plieva, K. Vanhoorelbeke, H. Deckmey, M. Tuncel, A.Tuncel, I.Y. Galaev, B. Mattiasson, J. Biotechnol., 131 (2007) 293–299.
24. P. Arvidsson, F.M. Plieva, I.N. Savina, V.I. Lozinsky, S. Fexby, L. Bulow, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A, 977 (2002) 27–38.
25. B. Garipcan, A. Denizli, Macromol. Biosci., 2 (2002) 135-144.
26. E. Valdman, L. Erijman, F.L.P. Pessoa, S.G.F. Leite, Process Biochem., 36 (2001) 869-873.
27. M. Odabaşı, G. Baydemir, M. Karataş, A. Derazshamshir, J. Appl. Polym. Sci., 116 (2010) 1306-1312.
28. P. Girot, E. Averty, I. Flayeuz, E. Boschetti, J. Chromatogr. B., 808 (2004) 25-33.
29. Z.P. Liang, Y.Q. Feng, Z.Y. Liang, S.X. Mang, Biochemical Eng. Journal., 24 (2005) 65-72.
30. H. Sun, B. Ge, S. Liu, H. Chen, J. Sep. Sci., 31 (2008) 1201.