Effectiveness of Organic Acid Treatments for Inhibition and Removal of E. coli Biofilms ​

Abstract

This study was carried out to evaluate the efficacies of organic acid citric, malic and gallic acids treatments at 1% and 2% concentrations for prevention and removal of E. coli biofilms. Antibiofilm effects of organic acids were compared with chlorine 100 ppm and 200 ppm on both microtitration plate and stainless steel coupons for 5, 10 and 20 min. Results indicated that citric acid treatments when compared to the chlorine tre- atments were effective for inhibition and removal of E. coli biofilms. On the other hand, gallic acid treatments were found to be more effective than malic acid treatments. On stainless steel surfaces, the inhibition and re- moval of biofilms were observed to be higher than those found on microtitration plates. Moreover, the inhibiti- on and removal ratios were calculated to be higher with increasing concentrations of sanitizers, on 24-h biofilm, on stainless steel coupons and with 20 min treatments. The results of this study indicates chlorine treatments could be replaced by organic acid treatments for inhibition and removal of biofilm formations of E. coli strains on different food contact surfaces. In addition, organic acid treatments are safe-to-use potential alternatives in industrial applications to chlorine treatments which is toxic to health and environment.

Keywords

• Biofilm inhibition,biofilm removal,E. coli,food contact surfaces,organic acids

E. coli Biyofilmlerinin Önlenmesi ve Ortadan Kaldırılmasında Organik Asit Uygulamalarının Etkileri

Abstract

B u çalışmada, E. coli biyofilmlerinin önlenmesi ve ortadan kaldırılmasında %1 ve %2 derişimlerdeki organik asit sitrik, malik ve gallik uygulamalarının etkileri araştırılmıştır. Organik asitlerin antibiyofilm etkileri klor uygulamaları 100 ppm ve 200 ppm ile 5, 10 ve 20 dakika süresince mikrotitrasyon plaklarında ve paslanmaz çelik kuponlarda karşılaştırılmıştır. Sonuçlar biyofilmlerin önlenme ve ortadan kaldırılmasında, klor uygulamaları ile karşılaştırıldığında, sitrik asit uygulamalarının etkili olduğunu göstermiştir. Gallik asit uygulamaları ise malik asit uygulamalarından daha etkili bulunmuştur. Çelik yüzeylerde, biyofilm önlenmesi ve ortadan kaldırılması oranları mikrotitrasyon plaklarından daha yüksek bulunmuştur. Ayrıca, biyofilm önlenme ve ortadan kaldırılma oranları, sanitizerların artan derişimlerinde, 24 saatlik biyofilm tabakası üzerinde, çelik yüzeylerde ve 20 dakikalık uygulamalarla daha yüksek değerlerde bulunmuştur. Çalışmanın sonuçları, farklı gıda ile temas eden yüzeylerde, E. coli biyofilmlerinin önlenmesi ve ortadan kaldırılmasında, organik asit uygulamalarının klor uygulamalarının yerini alabileceğini göstermektedir. Ayrıca organik asitler, endüstriyel uygulamalarda kullanılan sağlığa ve çevreye toksik etkili olan klorun kullanımının güvenli potansiyel alternatifleri olabilir

Keywords

• Biyofilm önlenmesi,biyofilm ortadan kaldırılması,E. coli,gıda ile temas eden yüzeyler,organik asitler

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