Immobilization of Thrombin to Alginate Gel and in vitro Application of Immobilized Thrombin

Abstract

Bleeding is one of the biggest problems of humanity. Blood loss in road traffic accidents and surgical operations is an important cause of death. In this study, a product which could decrease bleeding time was developed. The production process of developed product was very simple and the product has commercialization potential. Thrombin was immobilized to calcium alginate beads via ionic interactions. In this method, negative charged carboxylate groups of calcium alginate interacted ionically with the positive charge of thrombin. Optimization studies of thrombin immobilization were performed for increasing immobilization efficiency and it was found to be 4.1% at the end of optimization studies. The thrombin immobilized calcium alginate beads were used for the recalcification time test. The recalcification time is an indicator of bleeding time. The thrombin immobilized calcium alginate lowered recalcification time to 50 percent. Developed product has a potential to stop bleeding.

Keywords

• Thrombin,immobilization,calcium alginate,stop bleeding

Trombinin Alginat Jeline İmmobilizasyonu ve İmmobilize Trombinin In Vitro Uygulaması

Abstract

Kanama, insanlığın en büyük problemlerinden biridir. Trafik kazaları ve cerrahi operasyonlardaki kan kaybı, ölümlerin önemli bir sebebidir. Bu çalışmada, kanama süresini azaltabilecek bir ürün geliştirildi. Geliştirilen ürünün üretim süreci çok basittir. Ayrıca ürünün ticarileşme potansiyeli bulunmaktadır. Trombin, iyonik etkileşimler yoluyla kalsiyum aljinat boncuklara immobilize edildi. Bu yöntemde, kalsiyum aljinatın negatif yüklü karboksilat grupları, trombinin pozitif yükleriyle iyonik olarak etkileşime girdi. İmmobilizasyon verimini arttırmak için trombin immobilizasyonunun optimizasyon çalışmaları yapıldı. Optimizasyon çalışmalarının sonunda immobilizasyon verimi %4.1 olarak bulundu. Trombin immobilize kalsiyum aljinat boncukları, rekalsifikasyon zamanı testi için kullanıldı. Rekalsifikasyon zamanı, kanama süresi için bir göstergedir. Trombin immobilize kalsiyum aljinat, rekalsifikasyon zamanını %50’ye düşürdü. Geliştirilen ürün kanamayı durdurma potansiyeline sahiptir

Keywords

• Trombin,immobilizasyon,kalsiyum aljinat,kanamanın durdurulması

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