Utilization of Hydrophobic Interaction Affinity Nanosorbent For The Extraction Of Serotonin From Edible Plants

Abstract

The aim of this study was to evaluate the extraction performance of poly hydroxyethyl methacry-late-methacryloyl- L -tryptophan methyl ester poly HEMA–MAT ] nanostructures for serotonin from edible plants by a series of batch experiments. Average size of nanostructures was found as 100 nm with polydispersity index of 1.189 using zeta size analysis results. Maximum serotonin uptake capacity of the nanostructures was found to be 2901.4 ± 65.9 mg/g at pH 6.0. Applicabil-ity of this nanosorbent for the extraction of serotonin in banana and tomato before HPLC analysis was also studied and the level of serotonin was determined as 86.26 mg/g for banana and 36.02 mg/g for tomato samples.

Keywords

• Serotonin,nanostructures,Extraction,hydrophobic interaction chromatography

Yenilebilir Bitkilerden Serotoninin Özütlenmesi için Hidrofobik Etkileşim Afinite Nanosorbentinin Kullanımı

Abstract

B u çalışmanın amacı, bir seri kesikli sistem deneylerle poli hidroksietil metakrilat-metakriloil- L -triptofan metil ester poli HEMA-MAT ] nano-yapılarının yenilebilir bitkilerden serotonin için özütleme performansını değerlendirmektir. Polidispersiyon indeksi 1.189 olan nanoyapıların ortalama boyutu, zeta boyut analizi sonuçlarına göre 100 nmdir. Nanoyapıların maksimum serotonin adsorpsiyon kapasitesi, pH 6.0’da 2901.4±65.9 mg/g olarak bulunmuştur. Bu nanosorbentin, HPLC analizi öncesi muz ve domatesde serotonin özütlemesi için uygulanabilirliği de araştırılmış. Serotonin seviyesi muz için 86.26 mg/g, domates numuneleri için 36.02 mg/g olarak tespit edilmiştir

Keywords

• Serotonin,afinite nanosorbent,özütleme,hidrofobik etkileşim kromatografisi

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