Microbial Removal of Cyanide Compounds and Soil Cyanide by K. oxytoca

Abstract

In this study, K. oxytoca ATCC 13182 degraded potassium cyanide, potassium hexacyanoferrate II trihydra- te, potassium tetracyanonickelate II hydrate and sodium ferrocyanide decahydrate with the efficiencies of 100%, 87%, 78.5% and 27.5%, respectively. Additionally, optimal conditions for cyanide biodegradation were found as 30°C, 100 rpm and pH=7 at the concentration of 0.5 mM potassium cyanide in the biodegradation medium. Furthermore, K. oxytoca degraded potassium cyanide in the presence of different ions Mg, Ni, Co, Fe, Ca, Cr, As, Cu and Zn and as a result the growth amount of K. oxytoca decreased as the ion concentrations increased. It is also observed that 5:10 v:v concentration of sterile crude extract of K. oxytoca degraded 73.5% of the cyanide content in the biodegradation media in the first 24 hours. Finally, it is examined that 6 mgkg-1 and 240 mgkg-1 cyanide in soil samples were also degraded partially by K. oxytoca’s culture and sterile crude extract.

Keywords

• K. oxytoca,cyanide,sterile crude extract,Biodegradation

Siyanür Bileşiklerinin ve Topraktaki Siyanürün Klebsiella oxytoca ile Mikrobiyal Giderimi

Abstract

B u çalışmada, Klebsiella oxytoca ATCC 13182 suşunun potasyum siyanür, potasyum hekzasiyanoferrat II trihidrat, potasyum tetrasiyanonikelat II hidrat ve sodyum ferrosiyanür dekahidratı sırasıyla %100, %87, %78.5 ve %27.5 oranında bozunduğu belirlendi. Buna ek olarak, siyanür biyobozunma optimal koşullar 0.5 mM derişimde potasyum siyanür içeren biyobozunma besiyerinde, 30°C, 100 rpm ve pH 7.0 olarak bulundu. Ayrıca, K. oxytoca potasyum siyanürü farklı iyonların varlığında da Mg, Ni, Co, Fe, Ca, Cr, As, Cu ve Zn yıktığı gözlendi ve sonuç olarak K. oxytoca’nın üreme miktarının iyon derişimi arttıkça azalma gösterdiği belirlendi. K. oxytoca’nın 5:10 h:h derişimdeki steril kültür süpernatanının da ilk 24 saatte biyobozunma besiyerindeki siyanürün %73.5’ünü parçaladıgı gözlendi. Son olarak, K. oxytoca kültür ve steril kültür süpernatanının 6 mgkg1 ve 240 mgkg-1 siyanür içeren toprak örneklerindeki siyanürü de kısmen bozunmaya uğratabildiği belirlendi

Keywords

• Klebsiella oxytoca,siyanür,steril kültür özütü,biyobozunma

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