Effects of Salicylic Acid on Gaba Metabolism in Pepper Plants under Stress Conditions

Abstract

Salicylic acid SA is a potential plant hormone that enhances the capacity of plants to cope with various stress factors. Gamma-aminobutyric acid GABA is an important non-protein amino acid, which is increased by stress conditions. However, the interaction between SA and GABA is not well known. In this response the aim of this study was to evaluate the effects of SA on GABA metabolism in pepper plants Capsicum annuum L. under individual and combined salt NaCl and polyethylene glycol PEG induced osmotic stresses. Total chlorophyll content, the activities of glutamate dehydrogenase GDH, EC 1.4.1.4 and glutamate decarboxylase GAD, EC 4.1.1.15 , GABA content were analyzed in the leaves of pepper plants. Total chlorophyll content was decreased by combined salt and PEG-induced osmotic stresses. Individual SA, NaCl and PEG applications increased GDH activity. Under stress conditions, SA did not enhance GDH activity; however, GAD activity was increased within these conditions. Our results showed that salicylic acid application did not enhance GABA accumulation during individual and combined stress conditions. The dose and application process of SA could lead to this result. However further studies should be done to identify the interaction between SA and GABA under various stress conditions.

Keywords

• Capsicum annuum L.,GABA,tuz stresi,ozmotik stres,SA

Salisilik Asitin Stres Koşulları Altında Biber Bitkisinde Gaba Metabolizması Üzerine Etkisi

Abstract

S alisilik asit SA , bitkilerin çeşitli stres faktörlerine karşı korunma kapasitelerini artıran potansiyel bir bitki hormonudur. Gama-aminobütirik asit GABA stres koşullarında artan protein yapısında olmayan önemli bir amino asittir. Bununla birlikte, SA ve GABA arasındaki ilişki tam olarak bilinmemektedir. Bu çalışmanın amacı, tek ve birlikte uygulanan tuz NaCl ve polietilen glikol PEG ile indüklenen ozmotik stres koşullarında biber bitkisinde Capsicum annuum L. SA’nın GABA metabolizması üzerine etkilerinin incelenmesidir. Biber bitkisinin yapraklarında toplam klorofil miktarı, glutamat dehidrogenaz GDH, EC 1.4.1.4 ve glutamat dekarboksilaz GAD, EC 4.1.1.15 aktiviteleri ile GABA miktarı analiz edilmiştir. Toplam klorofil miktarı birlikte uygulanan tuz ve PEG’in indüklediği ozmotik stres koşullarında azalmıştır. SA, NaCl ve PEG’in tekli uygulamaları GDH aktivitelerini artırmıştır. Stres koşulları altında SA, GDH aktivitesini artırmamıştır; bununla birlikte GAD aktivitesi bu koşullar altında artmıştır. Sonuçlara göre, salisilik asit uygulaması tekli ve kombine stres koşullarında GABA birikimi artırmamıştır. SA’nın doz ve uygulama süreci bu sonuca neden olabilir. Bununla birlikte SA ve GABA arasındaki ilişkiyi belirlemek için farklı stres koşullarında çeşitli çalışmalar yapılmalıdır

Keywords

• Capsicum annuum L.,GABA,tuz stresi,ozmotik stres,SA

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