Promising Strain of Acinetobacter from Soil for Utilization of Gluconic Acid Production

Abstract

Gluconic acid, a food additive, is used in many foods to control acidity or binds metals such as calcium, iron. Acinetobacter sp. WR326, newly isolated from soil possesses high phosphate solubilizing activity and do not require pyrroloquinoline quinone PQQ for glucose dehydrogenase GDH activity as cofactor In this study the gluconic acid production potential of this bacterium was investigated. Firstly, Acinetobacter sp. WR326 was incubated in tricalcium phosphate medium TCP with varying glucose concentrations 100, 250, 500 mM , at a temperature of 30°C for 5 days 120 hours . The highest gluconic acid yield 59% was found at a glucose concentration of 100 mM. Then three different levels of gluconic acid addition to the medium 50, 100, 200 mM were tested. When Acinetobacter sp. WR326 strain was cultivated with a 100 mM glucose and 100 mM gluconic acid the yield increased to 95.27%. In any trials 2 -keto D-gluconic acid, causes problems in processing and purification of the gluconic acid, was not detected in the medium. As a conclusion, Acinetobacter sp. WR326 may be considered novel potential bacterial strain for gluconic acid production.

Keywords

• Acinetobacter sp. WR32,Gluconic acid,Fermentation,Phosphate solubilizing bacteria,Biotechnology

Topraktaki Gelecek Vaadeden Acinetobacter Suşunun Glukonik Asit Üretiminde Kullanımı

Abstract

G lukonik asit asitliği kontrol etmek veya kalsiyum, demir gibi metalleri bağlamak için gıdalarda kullanılan bir katkı maddesidir. Topraktan yeni izole edilen Acinetobacter sp. WR326, yüksek fosfat çözme aktivitesine sahiptir ve glukoz dehidrojenaz GDH aktivitesi için pyrroloquinoline quinone PQQ kofaktörüne gereksinim duymamaktadır. Bu çalışmada bu bakterinin glukonik asit üretme potansiyeli araştırılmıştır. İlk önce, Acinetobacter sp. WR326 farklı glukoz derişimlerde 100, 250, 500 mM 30ºC sıcaklıkta 5 gün boyunca 120 saat trikalsiyum fosfat TCP besiyerinde inkübe edilmiştir. En yüksek glukonik asit verimi %59 100 mM’lık glukoz derişimi içeren kültürde bulunmuştur. Sonra besiyerine glukonik asit ilavesi üç farklı derişimde 50, 100, 200 mM araştırılmıştır. Acinetobacter sp. WR326 suşu 100 mM glukoz ve 100 mM glukonik asit ilavesinde kültüre alındığında verim %95.27 oranına yükselmiştir. Hiçbir koşulda glukonik asidin işlenmesi ve saflaştırılmasında problemlere neden olan 2-keto D-glukonik asit tespit edilmemiştir. Sonuç olarak, Acinetobacter sp. WR326 suşunun glukonik asit üretiminde yeni potansiyel bakteri suşu olarak kullanılabileceği düşünülmektedir

Keywords

• Acinetobacter sp. WR32,glukonik asit,fermentasyon,fosfat çözücü bakteriler,biyoteknoloji

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