Preparation of E. Coli Minicells Containing RNA Polymerase Genes

Şükriye Baykan

Preparation of E. Coli Minicells Containing RNA Polymerase Genes

Abstract

AnF- strain of E. coli K-12 which produces DNA deficient minicells by an improper cell division has been used in developing methods to isolate plasmid DNA.I 2 By using sucrose gradient sedimentation, minicells can be easily separated from the parental cells that produce them. This E. coli mutant has a defect which is unable to synchronize DNA replication and cell division. Cell division occurs before DNA synthesis is finished, and' consequenty one of the daughter cells does not receive any DNA. These small and DNA-less cells are called minicells.I They are not viable cells, but they do maintain their biological activities for approximately 48 hrs, It is possible to prepare plasmids which contain any region of the bacterial chromosome, and these can be easily placed in minicells.

References

1. Adler, H. 1., Fischer, W. D., Cohen. A.. et al.: Miniature Escherichia coli cells deficient in DNA. Proc. Natl. Acad, Sci. U.S.A. 57 (1967), 321-326.
2. Inselburg, J.: Segregation into and replication of plasmid deoxyribonucleic into acid in chromosomeless segregants of Escherichia coli, J. Bacterial. 102 (1970), 642-647.
3. Roozen, K. J., Fenwick. R. G., Jr., and Curtiss, R., 3d.: Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12, J. Bacterial, 107 (1971), 21-33.
4. Rubenstein, K. E., Nass, M. M. and Cohen S. S.: Synthetic capabilities of plasmolyzed cells and spheroplasts of Escherichia coli, J. Bacteriol. 104 (1970), 443-452.
5. Levy, S. B.: Resistance of minicells to penic llin lysis: a method of obtaining large quantities of purified minicells, J. Bacterial, 103 (1970), 836-839.
6. Sippel A., and Hartman, G.: Mode of action of rafamycln on the DNA polymerase reaction, Biochim. Biophys. Acta 157 (1968), 218-129.
7. Austin, S. J., Tittawella, 1. P., Hayward, R. S., et al.: Amber mutations of Escherichia coli RNA polymerase, Nature (New BioI.) 232 (1971), 133-136.
8. Reichardt L., and Kaiser, A. D.: Control of lambda repressor synthesis, Proc, Natl. Acad. Sci. U.S.A. 68 (1971), 2185-2189).

Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Şükriye Baykan This is me

Publication Date

May 1, 1973

Submission Date

-

Acceptance Date

-

Published in Issue

Year 1973 Number: 2

IZ

https://izlik.org/JA75YX64XB

Cite

RIS / Bibtex

APA

Baykan, Ş. (1973). Preparation of E. Coli Minicells Containing RNA Polymerase Genes. Hacettepe Journal of Biology and Chemistry, 2, 14-19. https://izlik.org/JA75YX64XB

AMA

1.Baykan Ş. Preparation of E. Coli Minicells Containing RNA Polymerase Genes. HJBC. 1973;(2):14-19. https://izlik.org/JA75YX64XB

Chicago

Baykan, Şükriye. 1973. “Preparation of E. Coli Minicells Containing RNA Polymerase Genes”. Hacettepe Journal of Biology and Chemistry, nos. 2: 14-19. https://izlik.org/JA75YX64XB.

EndNote

Baykan Ş (May 1, 1973) Preparation of E. Coli Minicells Containing RNA Polymerase Genes. Hacettepe Journal of Biology and Chemistry 2 14–19.

IEEE

[1]Ş. Baykan, “Preparation of E. Coli Minicells Containing RNA Polymerase Genes”, HJBC, no. 2, pp. 14–19, May 1973, [Online]. Available: https://izlik.org/JA75YX64XB

ISNAD

Baykan, Şükriye. “Preparation of E. Coli Minicells Containing RNA Polymerase Genes”. Hacettepe Journal of Biology and Chemistry. 2 (May 1, 1973): 14-19. https://izlik.org/JA75YX64XB.

JAMA

1.Baykan Ş. Preparation of E. Coli Minicells Containing RNA Polymerase Genes. HJBC. 1973;:14–19.

MLA

Baykan, Şükriye. “Preparation of E. Coli Minicells Containing RNA Polymerase Genes”. Hacettepe Journal of Biology and Chemistry, no. 2, May 1973, pp. 14-19, https://izlik.org/JA75YX64XB.

Vancouver

1.Şükriye Baykan. Preparation of E. Coli Minicells Containing RNA Polymerase Genes. HJBC [Internet]. 1973 May 1;(2):14-9. Available from: https://izlik.org/JA75YX64XB