Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II

Serap Beyaztaş; Oktay Arslan

Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II

Abstract

The purpose of this study was to investigate the in vitro effects of four commonly used plant growth regulators PGRs : indole-3-acetic acid, indole-3-butyric acid, gibberellic acid and kinetin on human carbonic anhydrase I and II. Carbonic anhydrase I and II from human erythrocytes was purified by Sepharose-4B-L-tyrosine-1-sulfonamide affinity gel. IC50 values of the chemicals that caused inhibition were determined by means of activity percentage diagrams. The plant growth regulators used in this study effected the CA activity from human to various degrees. The concentrations of indole-3-acetic acid, indole-3-butyric acid and kinetin that inhibited 50% of the hCA I were 78.13 µM, 54.48 µM and 62.89 µM respectively. The concentrations of indole-3-acetic acid and indole-3-butyric acid that inhibited 50% of the hCA II were 75.47 µM and 38.05 µM, respectively. Conversely, the enzyme activity was increased by gibberellic acid and kinetin.

Keywords

Carbonic anhydrase I and II,indole-3-acetic acid,indole-3-butyric acid,gibberellic acid,kinetin,purification,Inhibition

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Details

Primary Language

English

Subjects

-

Journal Section

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Authors

Serap Beyaztaş This is me

Oktay Arslan This is me

Publication Date

December 1, 2009

Submission Date

-

Acceptance Date

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Published in Issue

Year 2009 Volume: 37 Number: 4

IZ

https://izlik.org/JA22XP44AD

Cite

RIS / Bibtex

APA

Beyaztaş, S., & Arslan, O. (2009). Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II. Hacettepe Journal of Biology and Chemistry, 37(4), 281-288. https://izlik.org/JA22XP44AD

AMA

1.Beyaztaş S, Arslan O. Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II. HJBC. 2009;37(4):281-288. https://izlik.org/JA22XP44AD

Chicago

Beyaztaş, Serap, and Oktay Arslan. 2009. “Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II”. Hacettepe Journal of Biology and Chemistry 37 (4): 281-88. https://izlik.org/JA22XP44AD.

EndNote

Beyaztaş S, Arslan O (December 1, 2009) Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II. Hacettepe Journal of Biology and Chemistry 37 4 281–288.

IEEE

[1]S. Beyaztaş and O. Arslan, “Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II”, HJBC, vol. 37, no. 4, pp. 281–288, Dec. 2009, [Online]. Available: https://izlik.org/JA22XP44AD

ISNAD

Beyaztaş, Serap - Arslan, Oktay. “Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II”. Hacettepe Journal of Biology and Chemistry 37/4 (December 1, 2009): 281-288. https://izlik.org/JA22XP44AD.

JAMA

1.Beyaztaş S, Arslan O. Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II. HJBC. 2009;37:281–288.

MLA

Beyaztaş, Serap, and Oktay Arslan. “Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 4, Dec. 2009, pp. 281-8, https://izlik.org/JA22XP44AD.

Vancouver

1.Serap Beyaztaş, Oktay Arslan. Affinity to Some Plant Growth Regulators on Human Erythrocytes Cytosolic Carbonic Anhydrase I and II. HJBC [Internet]. 2009 Dec. 1;37(4):281-8. Available from: https://izlik.org/JA22XP44AD