In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes
Abstract
In vitro effects of some purine analogues on human carbonic anhydrase CA, EC 4.2.1.1 were investigated in this study. Human erythrocyte CA-I and CA-II isozymes were purified using Sepharose-4B-aniline-sulfanilamide affinity gel chromatography. Specific activity and yields were determined as 904.08 EU mg-1, 59.86%; 6469.23 EU mg-1, 56.82%, respectively. The overall purification was approximately 105.74-fold for hCA-I and 756.64- fold for hCA-II. The inhibitory effects of different nucleoside analogues on CA activity were determined at low concentrations using the esterase method under in vitro conditions. I50 values for diprophylline, acyclovir, proxyphylline, aminophylline and caffeine were calculated from activity % -[I] as 4.12 μM, 5.58 μM, 9.96 μM, 19.17 mM, and 23.21 mM for hCA-I and 1.19 μM, 2.57 μM, 4.29 μM, 4.71 mM and 4.94 mM for hCA-II respectively. Additonally, the Lineweaver-Burk curves obtained were used for the determination of Ki and the inhibitor type for diprophylline, acyclovir and poxyphylline. Abbreviations: CA, carbonic anhydrase; hCA-I, human carbonic anhydrase I; hCA-II, human carbonic anhydrase II.
Keywords
References
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Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
December 1, 2009
Submission Date
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Acceptance Date
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Published in Issue
Year 2009 Volume: 37 Number: 4
APA
Şentürk, M., & Küfrevioğlu, Ö. İ. (2009). In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes. Hacettepe Journal of Biology and Chemistry, 37(4), 289-294. https://izlik.org/JA54HF58UR
AMA
1.Şentürk M, Küfrevioğlu Öİ. In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes. HJBC. 2009;37(4):289-294. https://izlik.org/JA54HF58UR
Chicago
Şentürk, Murat, and Ömer İrfan Küfrevioğlu. 2009. “In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes”. Hacettepe Journal of Biology and Chemistry 37 (4): 289-94. https://izlik.org/JA54HF58UR.
EndNote
Şentürk M, Küfrevioğlu Öİ (December 1, 2009) In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes. Hacettepe Journal of Biology and Chemistry 37 4 289–294.
IEEE
[1]M. Şentürk and Ö. İ. Küfrevioğlu, “In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes”, HJBC, vol. 37, no. 4, pp. 289–294, Dec. 2009, [Online]. Available: https://izlik.org/JA54HF58UR
ISNAD
Şentürk, Murat - Küfrevioğlu, Ömer İrfan. “In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes”. Hacettepe Journal of Biology and Chemistry 37/4 (December 1, 2009): 289-294. https://izlik.org/JA54HF58UR.
JAMA
1.Şentürk M, Küfrevioğlu Öİ. In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes. HJBC. 2009;37:289–294.
MLA
Şentürk, Murat, and Ömer İrfan Küfrevioğlu. “In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 4, Dec. 2009, pp. 289-94, https://izlik.org/JA54HF58UR.
Vancouver
1.Murat Şentürk, Ömer İrfan Küfrevioğlu. In Vitro Effects of Some Purine Analogue Drugs on Enzyme Activities of Carbonic Anhydrase Isozymes I and II from Human Erythrocytes. HJBC [Internet]. 2009 Dec. 1;37(4):289-94. Available from: https://izlik.org/JA54HF58UR