A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization

Year 2009, Volume: 37 Issue: 4 , 295 - 301 , 01.12.2009

Mihriban Şengör Nahit Aktaş

https://izlik.org/JA28HK87WH

Abstract

Enzymatic oxidative polymerization of phenol catalyzed by laccase enzyme extracted from Trametes versicolor ATCC 200801 in an aqueous solution containing acetone was investigated in a batch system. The effects of initial phenol and dissolved oxygen concentrations on the initial reaction rate of oxidative polymerization were experimented. An interactive kinetic model as a function of phenol and dissolved oxygen concentrations was developed for enzymatic polymerization and corresponding bio-kinetic parameters have been evaluated through a non-linear regression program so called SYSTAT 10.0 trial version. The bio-kinetic coefficient of the developed model, which are Vmax, Kmm, KmO2 and k2 were obtained as 0.029 mg DO /L.min, 66.58 mg/L, 0.89 mg/L, and 254 s-1 respectively. The activation energy of oxidative phenol polymerization was calculated as 21.175 kJ/mol.

Keywords

laccase , Phenol , Activation energy , Enzymatic polymerization , Wastewater treatment

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