A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization

Mihriban Şengör; Nahit Aktaş

A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization

Abstract

Enzymatic oxidative polymerization of phenol catalyzed by laccase enzyme extracted from Trametes versicolor ATCC 200801 in an aqueous solution containing acetone was investigated in a batch system. The effects of initial phenol and dissolved oxygen concentrations on the initial reaction rate of oxidative polymerization were experimented. An interactive kinetic model as a function of phenol and dissolved oxygen concentrations was developed for enzymatic polymerization and corresponding bio-kinetic parameters have been evaluated through a non-linear regression program so called SYSTAT 10.0 trial version. The bio-kinetic coefficient of the developed model, which are Vmax, Kmm, KmO2 and k2 were obtained as 0.029 mg DO /L.min, 66.58 mg/L, 0.89 mg/L, and 254 s-1 respectively. The activation energy of oxidative phenol polymerization was calculated as 21.175 kJ/mol.

Keywords

laccase,Phenol,Activation energy,Enzymatic polymerization,Wastewater treatment

References

1. Kazandjian, R. Z., Dordick, J. S., Klibanov, A. M., Biotechnol. Bioeng. 28:417-421, 1986.
2. Wright, H., Nicell, J.A., Biores. Technol. 70: 69-79, 1999.
3. Dordick, J.S., Marletta, M.A., Klibanov, A.M., Biotechnol. Bioeng. 30:31-36, 1987.
4. Kobayashi, S., Shoda, S., Uyama, H., in J.C. Salamone (Ed.), Polymeric Materials Encyclopedia, CRC Press, New York, 1996, Vol:3, p 2102.
5. Ajima, A., Yoshimoto, T., Takahashi, K., Tamaura, Y., Saito, Y., Inada, Y., Biotechnol. Lett. 7:303-306,1985.
6. Uyama, H., Kurioka, H., Komatasu, I., Sugihara, J., Kobayashi, S., Macromol. Reports. A 32:649-652, 1995.
7. Aizawa, M., Wang, L., in J.C. Salamone (Ed.), Polymeric Materials Encyclopedia, CRC Press, New York, 1996, Vol:3, p 2107.
8. Kobayashi, S., Shoda, S., Uyama, H., Adv. Polym. Sci. 121:1-29, 1995.

9. Kobayashi, S., Kurioka, H., Uyama, H., Macromol. Rapid Comm. 17:503-508, 1996.
10. Atlow, S.C., Bonadonna-Aparo, L., Klibanov, A.M., Biotechnol. Bioeng. 26:599-603, 1984.
11. Ikada, R., Sugihara, J., Uyama, H., Kobayashi, S., Macromol. 29:8702-8705, 1996.
12. Ikada, R., Uyama, H., Kobayashi, S., Macromol. 29: 3053-3054, 1996.
13. Aktaş, N., Kibarer, G., Tanyolaç, A., J. Chem. Technol. Biotechnol. 75:840-846, 2000.
14. Wu, Y., Taylor, K.E., Biswas, N., K Bewtra, J., J. Chem. Technol. Biotechnol. 74:519-526, 1999.
15. Dubey, S., Singh, D., Misra, R. A., Enzyme Microb. Tech. 23:432-437, 1998.
16. Draye, M., Czerwinski, K. R., Favre-Réguillon Foos, J., Guy, A., Lemaire, M., Sep. Sci. Technol. 35:1117-1132, 2000.
17. Aktaş, N., Tanyolaç, A., J. Biores. Technol. 87:209-214, 2003.
18. Coll, P.M., Fernandez-Abalos, J. M., Villanueva, J.R., Santamaria, R., Perez, P., Appl. Environ. Microbiol. 59: 2607-2613, 1993.
19. Lee, S.K., George, S.D., Antholine, W.E., Hedman, B., Hodgson, K.O., Solomon, E.I., J. Am. Chem. Soc. 124: 6180-6193, 2002.
20. Yaropolov, A.I., Skorobogatko, O.V., Vartanov, S.S., Varfolomeyev, S.D., Appl. Biochem. Biotechnol. 49:257- 263, 1994.
21. Kurniawati, S., Nicell, J., J. Chem. Technol. Biotechnol. 81:1198-1208, 2006.
22. Shuler, M.L., Kargi, F., Bioprocesses Engineering: Basic Concepts. Prentice Hall, New Jersey, 2002, Chp.3, p 57.
23. Systat® Version 10 (Demo) by Systat Incorporation 2000. 2902 Central st. Evanston,IL 60201 U.S.A.
24. Gianfreda, L., Xu, F., Bollog, M., Bioremediation J. 3:1- 26, 1999

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Mihriban Şengör This is me

Nahit Aktaş This is me

Publication Date

December 1, 2009

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2009 Volume: 37 Number: 4

IZ

https://izlik.org/JA28HK87WH

Cite

RIS / Bibtex

APA

Şengör, M., & Aktaş, N. (2009). A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization. Hacettepe Journal of Biology and Chemistry, 37(4), 295-301. https://izlik.org/JA28HK87WH

AMA

1.Şengör M, Aktaş N. A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization. HJBC. 2009;37(4):295-301. https://izlik.org/JA28HK87WH

Chicago

Şengör, Mihriban, and Nahit Aktaş. 2009. “A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization”. Hacettepe Journal of Biology and Chemistry 37 (4): 295-301. https://izlik.org/JA28HK87WH.

EndNote

Şengör M, Aktaş N (December 1, 2009) A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization. Hacettepe Journal of Biology and Chemistry 37 4 295–301.

IEEE

[1]M. Şengör and N. Aktaş, “A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization”, HJBC, vol. 37, no. 4, pp. 295–301, Dec. 2009, [Online]. Available: https://izlik.org/JA28HK87WH

ISNAD

Şengör, Mihriban - Aktaş, Nahit. “A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization”. Hacettepe Journal of Biology and Chemistry 37/4 (December 1, 2009): 295-301. https://izlik.org/JA28HK87WH.

JAMA

1.Şengör M, Aktaş N. A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization. HJBC. 2009;37:295–301.

MLA

Şengör, Mihriban, and Nahit Aktaş. “A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 4, Dec. 2009, pp. 295-01, https://izlik.org/JA28HK87WH.

Vancouver

1.Mihriban Şengör, Nahit Aktaş. A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization. HJBC [Internet]. 2009 Dec. 1;37(4):295-301. Available from: https://izlik.org/JA28HK87WH