A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization

Year 2009, Volume: 37 Issue: 4, 295 - 301, 01.12.2009

Mihriban Şengör Nahit Aktaş

Abstract

Enzymatic oxidative polymerization of phenol catalyzed by laccase enzyme extracted from Trametes versicolor ATCC 200801 in an aqueous solution containing acetone was investigated in a batch system. The effects of initial phenol and dissolved oxygen concentrations on the initial reaction rate of oxidative polymerization were experimented. An interactive kinetic model as a function of phenol and dissolved oxygen concentrations was developed for enzymatic polymerization and corresponding bio-kinetic parameters have been evaluated through a non-linear regression program so called SYSTAT 10.0 trial version. The bio-kinetic coefficient of the developed model, which are Vmax, Kmm, KmO2 and k2 were obtained as 0.029 mg DO /L.min, 66.58 mg/L, 0.89 mg/L, and 254 s-1 respectively. The activation energy of oxidative phenol polymerization was calculated as 21.175 kJ/mol.

Keywords

laccase , Phenol , Activation energy , Enzymatic polymerization , Wastewater treatment

References

• 1. Kazandjian, R. Z., Dordick, J. S., Klibanov, A. M., Biotechnol. Bioeng. 28:417-421, 1986.
• 2. Wright, H., Nicell, J.A., Biores. Technol. 70: 69-79, 1999.
• 3. Dordick, J.S., Marletta, M.A., Klibanov, A.M., Biotechnol. Bioeng. 30:31-36, 1987.
• 4. Kobayashi, S., Shoda, S., Uyama, H., in J.C. Salamone (Ed.), Polymeric Materials Encyclopedia, CRC Press, New York, 1996, Vol:3, p 2102.
• 5. Ajima, A., Yoshimoto, T., Takahashi, K., Tamaura, Y., Saito, Y., Inada, Y., Biotechnol. Lett. 7:303-306,1985.
• 6. Uyama, H., Kurioka, H., Komatasu, I., Sugihara, J., Kobayashi, S., Macromol. Reports. A 32:649-652, 1995.
• 7. Aizawa, M., Wang, L., in J.C. Salamone (Ed.), Polymeric Materials Encyclopedia, CRC Press, New York, 1996, Vol:3, p 2107.
• 8. Kobayashi, S., Shoda, S., Uyama, H., Adv. Polym. Sci. 121:1-29, 1995.
• 9. Kobayashi, S., Kurioka, H., Uyama, H., Macromol. Rapid Comm. 17:503-508, 1996.
• 10. Atlow, S.C., Bonadonna-Aparo, L., Klibanov, A.M., Biotechnol. Bioeng. 26:599-603, 1984.
• 11. Ikada, R., Sugihara, J., Uyama, H., Kobayashi, S., Macromol. 29:8702-8705, 1996.
• 12. Ikada, R., Uyama, H., Kobayashi, S., Macromol. 29: 3053-3054, 1996.
• 13. Aktaş, N., Kibarer, G., Tanyolaç, A., J. Chem. Technol. Biotechnol. 75:840-846, 2000.
• 14. Wu, Y., Taylor, K.E., Biswas, N., K Bewtra, J., J. Chem. Technol. Biotechnol. 74:519-526, 1999.
• 15. Dubey, S., Singh, D., Misra, R. A., Enzyme Microb. Tech. 23:432-437, 1998.
• 16. Draye, M., Czerwinski, K. R., Favre-Réguillon Foos, J., Guy, A., Lemaire, M., Sep. Sci. Technol. 35:1117-1132, 2000.
• 17. Aktaş, N., Tanyolaç, A., J. Biores. Technol. 87:209-214, 2003.
• 18. Coll, P.M., Fernandez-Abalos, J. M., Villanueva, J.R., Santamaria, R., Perez, P., Appl. Environ. Microbiol. 59: 2607-2613, 1993.
• 19. Lee, S.K., George, S.D., Antholine, W.E., Hedman, B., Hodgson, K.O., Solomon, E.I., J. Am. Chem. Soc. 124: 6180-6193, 2002.
• 20. Yaropolov, A.I., Skorobogatko, O.V., Vartanov, S.S., Varfolomeyev, S.D., Appl. Biochem. Biotechnol. 49:257- 263, 1994.
• 21. Kurniawati, S., Nicell, J., J. Chem. Technol. Biotechnol. 81:1198-1208, 2006.
• 22. Shuler, M.L., Kargi, F., Bioprocesses Engineering: Basic Concepts. Prentice Hall, New Jersey, 2002, Chp.3, p 57.
• 23. Systat® Version 10 (Demo) by Systat Incorporation 2000. 2902 Central st. Evanston,IL 60201 U.S.A.
• 24. Gianfreda, L., Xu, F., Bollog, M., Bioremediation J. 3:1- 26, 1999