Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source
Abstract
The purpose of this study is to examine levels of polyphenol oxidase PPO activities in the crude extracts of seventeen different herbal tissues Banana peel, Jerusalem artichoke, black grape, buckthorn, fresh bean, pea, cactus apple, aloe vera, cabbage turnip graim, cabbage turnip vegetable, celeriac, quince, artichoke, aubergine, Trabzon palm, dry carob with spectrophotometric method. The three herbal tissues Anamur banana peel, Jerusalem artichoke and fresh broad bean were found to have higher PPO activities than other tissues, 638, 3360, 3677 U/min.mg protein, respectively. Biologic oxygen biosensors were prepared by cross-linked thin film immobilization method, in the presence of gelatin and glutaraldehyde, with three herbal tissues used as biomaterials and these biosensors were used for detection of phenolic compounds via determining the concentration of consumed oxygen throughout the reaction medium. The typical calibration curves for the Anamur banana peel, Jerusalem artichoke and fresh broad bean based sensors revealed a linear range of 5-40 μM, 2-10 μM, 10-100 μM, respectively. In reproducibility studies, variation coefficiets CV and standard deviations SD were calculated as 1.4%-0.3294 μM, 2%-0.8392 μM and 1%-0.0746 μM, respectively.
Keywords
References
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Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
December 1, 2009
Submission Date
-
Acceptance Date
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Published in Issue
Year 2009 Volume: 37 Number: 4
APA
Sağıroglu, A., Özcan, H. M., & Hasancebi, Ö. (2009). Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source. Hacettepe Journal of Biology and Chemistry, 37(4), 303-312. https://izlik.org/JA97ZC48FC
AMA
1.Sağıroglu A, Özcan HM, Hasancebi Ö. Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source. HJBC. 2009;37(4):303-312. https://izlik.org/JA97ZC48FC
Chicago
Sağıroglu, Ayten, Hakkı Mevlüt Özcan, and Özhan Hasancebi. 2009. “Scanning of Some Herbal Tissues to Be Used With Biosensors As Polyphenol Oxidase Enzyme Source”. Hacettepe Journal of Biology and Chemistry 37 (4): 303-12. https://izlik.org/JA97ZC48FC.
EndNote
Sağıroglu A, Özcan HM, Hasancebi Ö (December 1, 2009) Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source. Hacettepe Journal of Biology and Chemistry 37 4 303–312.
IEEE
[1]A. Sağıroglu, H. M. Özcan, and Ö. Hasancebi, “Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source”, HJBC, vol. 37, no. 4, pp. 303–312, Dec. 2009, [Online]. Available: https://izlik.org/JA97ZC48FC
ISNAD
Sağıroglu, Ayten - Özcan, Hakkı Mevlüt - Hasancebi, Özhan. “Scanning of Some Herbal Tissues to Be Used With Biosensors As Polyphenol Oxidase Enzyme Source”. Hacettepe Journal of Biology and Chemistry 37/4 (December 1, 2009): 303-312. https://izlik.org/JA97ZC48FC.
JAMA
1.Sağıroglu A, Özcan HM, Hasancebi Ö. Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source. HJBC. 2009;37:303–312.
MLA
Sağıroglu, Ayten, et al. “Scanning of Some Herbal Tissues to Be Used With Biosensors As Polyphenol Oxidase Enzyme Source”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 4, Dec. 2009, pp. 303-12, https://izlik.org/JA97ZC48FC.
Vancouver
1.Ayten Sağıroglu, Hakkı Mevlüt Özcan, Özhan Hasancebi. Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source. HJBC [Internet]. 2009 Dec. 1;37(4):303-12. Available from: https://izlik.org/JA97ZC48FC