Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase

Esrin Akman; Vedat Türkoğlu; Ismail Celik

Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase

Abstract

In this study, acethylcholinesterase was purified from liver and brain of Van Lake fish Chalcalburnus Tarichii P.1811 by using affinity chromatography technique. The purification folds were determined as 142 and 344 for brain and liver, respectively. The purity of enzyme was controlled with sodium dodecylsulfate-polyacrylamide gel electrophoresis SDSPAGE . The optimal pH was determined as 7.5-8.5 for brain and liver, respectively. On the other hand, the optimal temperature was determined as 35oC for both liver and brain. Also, the highest activities were observed in concentration of 0.2 M NH4 2SO4 as ionic strength for both liver and brain.

Keywords

purification,characterization,Acethylcholinesterase,Chalcalburnus Tarichii,Fish

References

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Esrin Akman This is me

Vedat Türkoğlu This is me

Ismail Celik This is me

Publication Date

December 1, 2009

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2009 Volume: 37 Number: 4

IZ

https://izlik.org/JA36WP86LM

Cite

RIS / Bibtex

APA

Akman, E., Türkoğlu, V., & Celik, I. (2009). Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase. Hacettepe Journal of Biology and Chemistry, 37(4), 331-336. https://izlik.org/JA36WP86LM

AMA

1.Akman E, Türkoğlu V, Celik I. Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase. HJBC. 2009;37(4):331-336. https://izlik.org/JA36WP86LM

Chicago

Akman, Esrin, Vedat Türkoğlu, and Ismail Celik. 2009. “Purification and Characterization of Van Lake Fish Chalcalburnus Tarichii P.1811 Liver and Brain Acethylcholinesterase”. Hacettepe Journal of Biology and Chemistry 37 (4): 331-36. https://izlik.org/JA36WP86LM.

EndNote

Akman E, Türkoğlu V, Celik I (December 1, 2009) Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase. Hacettepe Journal of Biology and Chemistry 37 4 331–336.

IEEE

[1]E. Akman, V. Türkoğlu, and I. Celik, “Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase”, HJBC, vol. 37, no. 4, pp. 331–336, Dec. 2009, [Online]. Available: https://izlik.org/JA36WP86LM

ISNAD

Akman, Esrin - Türkoğlu, Vedat - Celik, Ismail. “Purification and Characterization of Van Lake Fish Chalcalburnus Tarichii P.1811 Liver and Brain Acethylcholinesterase”. Hacettepe Journal of Biology and Chemistry 37/4 (December 1, 2009): 331-336. https://izlik.org/JA36WP86LM.

JAMA

1.Akman E, Türkoğlu V, Celik I. Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase. HJBC. 2009;37:331–336.

MLA

Akman, Esrin, et al. “Purification and Characterization of Van Lake Fish Chalcalburnus Tarichii P.1811 Liver and Brain Acethylcholinesterase”. Hacettepe Journal of Biology and Chemistry, vol. 37, no. 4, Dec. 2009, pp. 331-6, https://izlik.org/JA36WP86LM.

Vancouver

1.Esrin Akman, Vedat Türkoğlu, Ismail Celik. Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase. HJBC [Internet]. 2009 Dec. 1;37(4):331-6. Available from: https://izlik.org/JA36WP86LM