Microbial Transformation of 3,3-Dimethylcyclohexyl methyl ketone and Antimicrobial Evaluation

Year 2022, , 263 - 267, 01.08.2022

Özge Özşen Batur İsmail Kıran Kemal Başer Fatih Demirci

https://doi.org/10.15671/hjbc.982977

Cited By: 1

Abstract

3,3-Dimethylcyclohexyl methyl ketone (Herbac®) is a fine fragrance compound used in the cosmetic industry. In this study, the biological derivatisation of the substrate 3,3-dimethylcyclohexyl methyl ketone by 18 fungal cultures was carried out to obtain new derivatives. Among the evaluated, the plant fungus Aspergillus niger ATCC 10549, biotransformed the substrate to the 4-hydroxy derivative in 19% yield. The structure was determined by using NMR and GC-MS spectroscopic methods. In addition, the antimicrobial activity of Herbac® with the metabolite against a panel of pathogenic microbial strains were evaluated using in vitro microdilution. The results showed that the metabolite was relatively more susceptible compared to the substrate Herbac®.

Keywords

antimicrobial activity, Aspergillus niger, Biotransformation, Fragrance compounds

Supporting Institution

Scientific and Technological Research Council of Turkey

Project Number

TEYDEB- 7110249

Thanks

Financial support from the Scientific and Technological Research Council of Turkey (Grant no: TEYDEB- 7110249) is kindly acknowledged. The authors express their thamks to International Flavors & Fragrances (IFF) Co., USA for providing HERBAC and Badebio Biotechnology Ltd. its infrastructure.

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