Simultaneous Determination of Three Phenolic Compounds in Water Samples by Pre-column Derivatization Coupled with Reversed-Phase High Performance Liquid Chromatography

Year 2018, Volume: 46 Issue: 2, 147 - 157, 03.06.2018

Adem Asan M.umut Konanç Bedia Akmeşe

Abstract

I
n this study, a sensitive and accurate method for simultaneous separation and determination of three
phenolic compounds (phenol, m-cresol and resorcinol) in water by revesed-phase high performance
liquid chromatography using uv-visible detection has been described. Pre-column derivatization with
4-aminoantipyrine is used for the seperation and determination phenol, m-cresol and resorcinol in water. The
derivatives formed within 5 min were extracted with chloroform and then analyzed by liquid chromatography
with UV-visible detection at 440 nm. Chromatographic separation was performed using a reversed-phase
column and acetonitrile-water (45:55%, v/v) as the mobile phase. The three derivatives were elueted in 13 min.
The detection limits of phenol, m-cresol and resorcinol in a standard water sample were between 0.07 and 0.09
μg.L-1 for 100 mL respectively. The recoveries of the derivatives from pure water were betweeen 97.1-102.3%
within relative standard deviations of 2.3-4.7%. The method was applied to the analysis of phenols in different
water samples.

Keywords

Phenols, derivatization, reversed-phase liquid chromatography, 4-aminoantipyrine

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