Detection of Lipase Production from Newly Isolated Trichoderma Citrinoviride

Year 2018, Volume: 46 Issue: 2, 229 - 235, 03.06.2018

Muhammed Hasan Akyıl Nilüfer Cihangir

Abstract

The production of lipase is aimed from a new fungal source in this study. Fungus was isolated from soil
locality Kocaeli. It was determined as Trichoderma citrinoviride by the analysis of 18S rRNA sequence.
Various parameters and media components were investigated for production of lipase. Glucose and peptone
were found to be most suitable carbon and nitrogen source, respectively. To determine the suitable oil as
carbon source, various oils were added to the production medium. Olive oil was found to be the optimal oil for
lipase production from Trichoderma citrinoviride. pH 5.5, temperature 30°C and incubation time for 4 days
were found to be optimal incubation conditions for lipase production. We also determined lipase yield from
Trichoderma citrinoviride which is produced in molasses medium as an alternative carbon source

Keywords

Lipase, Trichoderma citrinoviride, microbial lipase production

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