Characterization of Local Trichoderma spp. as Potential Bio-Control Agents, Screening of in vitro Antagonistic Activities and Fungicide Tolerance

Year 2018, Volume: 46 Issue: 2, 247 - 261, 03.06.2018

Şengül Alpay Karaoğlu Arif Bozdeveci Necla Pehlivan

Abstract

Enlightening effects of biocontrol agents as Trichoderma spp. to provide disease control by combating pathogens
is an important alternative in agricultural crop production. To that end, 9 species of Hypocrea/
Trichoderma having green ascospores isolated from the soil that tea plants cultivated in are identified by ITS
sequences and were found to be close relative of Trichoderma sect. Pachybasium (77%). Ethyl acetate extracts
of fungal isolates exhibited the antimicrobial activity against to Vibrio sp, Serratia marcescens, Mycobacterium
smegmatis and Bacillus cereus but no antifungal activity was detected. The highest level of inhibitory activity
was observed against to M. smegmatis by Trichoderma harzianum ID4A, ID4B and ID6B. In dual culture test, all
Trichoderma strains were found to be showing the highest inhibitory activity against to the plant pathogens
Botrytis cinerea, Sclerotonia sclerotiorum and Rhizoctonia solani (AG3), but relatively low activity against to
the entomopathogen fungi. Volatile metabolites of Trichoderma spp. caused maximum reduction in mycelial
growth and sclerotial production. Tested strains showed the highest tolerance to the fungicide Dikozin, whereas
the lowest tolerance was against to the Captan regardless of the dosage.

Keywords

Trichoderma, bio-control agent, Antimicrobial activity, Fungicide

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