The Effects of Juvenile Hormone Analogue, Fenoxycarb on the Last Instar Testes of Bombyx mori

Year 2018, Volume: 46 Issue: 2, 297 - 305, 03.06.2018

Gamze Turgay-izzetoğlu , Asiye Pak Tuğba Zülfikaroğlu Taylan K. Öztürk

Abstract

The development and metamorphosis of insects is regulated by juvenile hormone (JH) and ecdysone hormone,
which are worked antagonist. Fenoxycarb is an effective juvenile hormone analogue on many insects. In this
study, the effect of fenoxycarb on the last larval stage Bombyx mori testes was investigated. Male larvae on
the 6th day of the last instar are divided into 2 groups. 1 ng of fenoxycarb dissolved in acetone was topically
applied to larvae in the treatment group and the remaining 18 larvae were used as the control group. Control
and treated groups were followed to until pupation from day when they showed cocoon spinning behavior. After
dissection, several of testes were entreated routine histological processes and photographed. In treatment
group, it was seen that fenoxycarb reduced spermatogonium formation by suppressing ecdysone release and
caused a decrease in mature sperm. Remaining testes are used to the analysis of total carbohydrate and
glycogen content. In conclusion, fenoxycarb has the reducing effect on spermatogenesis and it can cause to
decrease of total carbohydrate content because of stress in insects. These histological and biochemical results
may be in support of the use of fenoxycarb as an insect growth regulator (IGR) for harmful insects.

Keywords

Fenoxycarb, histology, spectrophotometry, testis of Bombyx

References

• O. Parlak, İpekböceği Biyolojisi, Ege Üniversitesi Basımevi, İzmir, No: 171 (2001) 127s ISBN 975-483- 485-7.
• C. Gillott, Entomology, 3rd edit., University of Saskatchewan Saskatoon, Saskatchewan, Canada (2005) 831 p.
• M.J. Klowden, In physiological systems in insects, Chapter 4 – Reproductive Systems, 2. edit, University of Idaho, Moscow Idaho (2008) 181-238.
• N. Kılınçer, Ş. Bayram, Böceklerde üreme sistemleri, Ankara Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü (1999) 59s.
• J.L. Nation, Insect physiology and biochemistry, 2nd edit., Department of Entomology and Nematology University of Florida, U.S.A (2008) 500 p, ISBN 978- 1-4200-6177-2.
• B. Yılmaz, Omurgasızlarda hormonlar, Ankara Üniv. Vet. Fak. Derg., 35 (1988) 218-226.
• J. Devillers, Juvenile hormones and juvenoids, 3rd edit., CTIS-centre de traitement de l’information scientifique rillieux la pape, France (2013) 410 p, ISBN 978-1-4665-1322.
• E. Göncü, O. Parlak, The influence of juvenile hormone analogue, fenoxycarb on the midgut remodeling in Bombyx mori (L., 1758) (Lepidoptera: Bombycidae) during larval-pupal metamorphosis, Turkish J. Ent., 35 (2011) 179-194.
• E. Batır, E. Göncü, and O. Parlak, Ecdysone receptor B1 in Bombyx mori L. (Lepidoptera:Bombycidae) prothoracic gland under various organ culture conditions, IUFS J. Bio., 71 (2012) 31-42.
• G. Turgay İzzetoğlu, A. Öber, Histological investigation of the rectal sac in Bombyx mori L., Turk J Zool., 35 (2011) 213-221.
• G. Turgay İzzetoğlu, A. Öber, Total proteolytic activity on the pupal rectal sac of Bombyx mori L. (Lepidoptera: Bombycidae), Hacettepe J. Bio. Chem., 41 (2013) 67-72.
• M. Kamimura, M. Kiuchi, Effects of a juvenile hormone analog, fenoxycarb, on 5th stadium larvae of the silkworm, Bomyx mori (Lepidoptera:Bombycidae), Appl. Ent Zool., 33 (1998) 333-338.
• J.K. Presnell, M.P. Schreibman, Humason’s animal tissue techniques, 5th edition, The Johns Hopkins University Press (1997) 572p, ISBN 0-8018-5401-6.
• A. Öber, Zoolojide Laboratuvar Teknikleri, 3. Baskı, Ege Üniversitesi Basımevi Bornova İzmir, No: 183 (2009) 209s. ISBN 978-975-483-824-4.
• E. Van Handel, Rapid determination of glycogen and sugars in mosquitoes, J. Am. Mosq. Control Assoc., 1 (1985) 199-301.
• M. Kamimura, Effects of a juvenile hormone analogue, fenoxycarb, on larval growth of the silkworm, Bombyx mori (Lepidoptera: Bombycidae), JSAEZ, 30 (1995) 487-489.
• S.G. Dedos, H. Fugo, Induction of dauer larvae by application of fenoxycarb early in the 5th instar of the silkworm, Bombyx mori, J. Insect Physiol., 45 (1999) 769-775.
• M.G. Leonardi, S. Cappellozza, L. Cappellozza, P. Lanne, P. Parentis, B. Giordana, Effects of the topical application of an insect growth regulator (fenoxycarb) on some physiological parameters in the fifth instar larvae of the silkworm Bombyx mori, Comp. Biochem. Physiol., 113 (1996) 361-365.
• O. Parlak, S. Sakurai, M. Kaya, T. Ohtaki, Content and possible role of ecdysterids in the larval ovary of the silkworm, Bombyx mori, Invertebrate Reprod. Develop., 21 (1992) 1-6.
• R. Pant, S. Kumar, Metabolic fate of carbohydrates and lipids during moulting of Philosamia ricini (Lepidoptera: Saturnidae), Insect Biochem., 9 (1979) 577-582.
• D. Mandal, D.K. Chaudhuri, Studies on carbohydrate, protein and lipid levels in normal and stress conditions in fat body and integument as compared to whole body during development of rice moth, Corcyra cephalonica (ST), Insect Sci. Applic., 13 (1992) 121–128.
• M.L.M. Garcia, R.P. Mello, B.C.M. Mello, Effect of Precocene II, Ecdysone and juvenile hormone on the glycogen concentration in pupae of Stomoxys calcitrans (Dipter Muscidae), Mem. Inst. Oswalda Cruz, 83 (1988) 451–454.
• K. Fotouhi, M.M. Fazel, A. Kavousi, Effects of pyriproxyfen on bioenergetic resources of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), Türk. Entomol. Derg., 39 (2015) 11–22.
• G.J.P. Singh, Hemolymph carbohydrate and lipid mobilization in Locusta migratoria in relation to the progress of poisoning following bioresmethrin treatment, Pest. Biochem. Phys., 25 (1986) 264–269.