The Effects of Juvenile Hormone Analogue, Fenoxycarb on the Last Instar Testes of Bombyx mori

Year 2018, Volume: 46 Issue: 2, 297 - 305, 03.06.2018

Gamze Turgay-izzetoğlu Asiye Pak Tuğba Zülfikaroğlu Taylan K. Öztürk

Abstract

The development and metamorphosis of insects is regulated by juvenile hormone (JH) and ecdysone hormone,
which are worked antagonist. Fenoxycarb is an effective juvenile hormone analogue on many insects. In this
study, the effect of fenoxycarb on the last larval stage Bombyx mori testes was investigated. Male larvae on
the 6th day of the last instar are divided into 2 groups. 1 ng of fenoxycarb dissolved in acetone was topically
applied to larvae in the treatment group and the remaining 18 larvae were used as the control group. Control
and treated groups were followed to until pupation from day when they showed cocoon spinning behavior. After
dissection, several of testes were entreated routine histological processes and photographed. In treatment
group, it was seen that fenoxycarb reduced spermatogonium formation by suppressing ecdysone release and
caused a decrease in mature sperm. Remaining testes are used to the analysis of total carbohydrate and
glycogen content. In conclusion, fenoxycarb has the reducing effect on spermatogenesis and it can cause to
decrease of total carbohydrate content because of stress in insects. These histological and biochemical results
may be in support of the use of fenoxycarb as an insect growth regulator (IGR) for harmful insects.

Keywords

Fenoxycarb, histology, spectrophotometry, testis of Bombyx

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