A New Approach for Quorum Sensing System in Several Halophilic Bacteria Isolated from Salt Lake in Central Anatolia

Year 2018, Volume: 46 Issue: 2, 177 - 185, 03.06.2018

Demet Erdönmez Kübra Erkan Türkmen Nilüfer Aksöz

Abstract

Quorum sensing (qs) is the system that microorganisms use to improve common behavior with another
population member in their environment. Salt Lake in Turkey that is considered as an extreme environment
due to its salt concentration, teems in plankton and bacteria. Especially, it is an important field for halophilic
bacteria because it provides suitable growth conditions. In this study, it was determined that 20 halophilic
bacteria strain isolated from Salt Lake could produce qs signal molecules which are N-acyl homoserine lactone
(HSL) derivatives. For detection of these bacterial signal molecules, thin layer chromatography was utilized by
using Chromobacterium violaceum cv026 and Agrobacterium tumefaciens A136 that are biosensor bacteria
strains. Genomic analysis of strains that produce signal molecules was carried out via 16s rRNA. Halobacterium
salinarium, Salicola sp., Halovibrio, and Halomonas were found to be common in Salt Lake. It was also observed
that these strains could produce C6-HSL and C8-HSL signaling molecules and produce biofilms.

Keywords

Homoserine lactone, quorum sensing, halophilic bacteria, Salt Lake

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