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Year 2018, Volume: 46 Issue: 1, 35 - 41, 01.03.2018

Abstract

References

  • K. Takahashi, S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors, Cell, 126 (2006) 663-76.
  • I.H. Park, N. Arora, H. Huo, N. Maherali, T. Ahfeldt, A. Shimamura, M.W. Lensch, C. Cowan, K. Hochedlinger, G.Q. Daley, Disease-specific induced pluripotent stem cells, Cell, 134 (2008) 877-86.
  • F.T. Merkle, K. Eggan, Modeling human disease with pluripotent stem cells: from genome association to function, Cell. Stem Cell, 12 (2013) 656-68.
  • J. Sng, T. Lufkin, Emerging stem cell therapies: treatment, safety, and biology, Stem Cells Int., 2012 (2012) 521343.
  • M.A. Dawson, T. Kouzarides, Cancer epigenetics: from mechanism to therapy, Cell, 150 (2013) 12-27.
  • S.B. Baylin, J.E. Ohm, Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction?, Nat. Rev. Cancer, 6 (2006) 107-116.
  • A.P. Feinberg, B. Tycko, The history of cancer epigenetics, Nat. Rev. Cancer, 4 (2004) 143-153.
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  • C.A. Gifford, Michael J. Ziller, H. Gu, C. Trapnell, J. Donaghey, A. Tsankov, A.K. Shalek, D.R. Kelley, A.A. Shishkin, R. Issner, X. Zhang, M. Coyne, J.L. Fostel, L. Holmes, J. Meldrim, M. Guttman, C. Epstein, H. Park, O. Kohlbacher, J. Rinn, A. Gnirke, E.S. Lander, B.E. Bernstein, A. Meissner, Transcriptional and epigenetic dynamics during specification of human embryonic stem cells, Cell, 153 (2013) 1149-1163.
  • W. Xie, M.D. Schultz, R. Lister, Z. Hou, N. Rajagopal, P. Ray, J. W. Whitaker, S. Tian, R.D. Hawkins, D. Leung, H. Yang, T. Wang, Ah Y. Lee, S.A. Swanson, J. Zhang, Y. Zhu, A. Kim, J.R. Nery, Mark A. Urich, S. Kuan, C.-a. Yen, S. Klugman, P. Yu, K. Suknuntha, N.E. Propson, H. Chen, L.E. Edsall, U. Wagner, Y. Li, Z. Ye, A. Kulkarni, Z. Xuan, W.-Y. Chung, N.C. Chi, J.E. AntosiewiczBourget, I. Slukvin, R. Stewart, M.Q. Zhang, W. Wang, J.A. Thomson, J.R. Ecker, B. Ren, Epigenomic analysis of multilineage differentiation of human embryonic stem cells, Cell, 153 (2013) 1134-1148.
  • J. Zhu, M. Adli, J.Y. Zou, G. Verstappen, M. Coyne, X. Zhang, T. Durham, M. Miri, V. Deshpande, P.L. De Jager, D.A. Bennett, J.A. Houmard, D.M. Muoio, T.T. Onder, R. Camahort, C.A. Cowan, A. Meissner, C.B. Epstein, N. Shoresh B.E. Bernstein, Genome-wide chromatin state transitions associated with developmental and environmental Cues, Cell, 152 (2013) 642-654.
  • B. Papp, K. Plath, Epigenetics of reprogramming to induced pluripotency, Cell, 152 (2013) 1324-43.
  • R.P. Koche, Z.D. Smith, M. Adli, H. Gu, M. Ku, A. Gnirke, B.E. Bernstein, A. Meissner, Reprogramming factor expression initiates widespread targeted chromatin remodeling, Cell. Stem. Cell, 8 (2013) 96-105.
  • A. Yilmazer, I. de Lázaro, H. Taheri, Reprogramming cancer cells: a novel approach for cancer therapy or a tool for disease-modeling?, Cancer Letters, 369 (2015) 1-8.

Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors

Year 2018, Volume: 46 Issue: 1, 35 - 41, 01.03.2018

Abstract

The generation of induced pluripotent stem (iPS) cells from somatic cells showed that cell fate could be
manipulated by simply introducing a few transcription factors. The differentiation potential of iPS cells has
uncovered a wide range of potential applications, including disease modeling, drug screening and regenerative
therapy. In this study, we aimed to induce reprogramming of human melanocytes and melanoma cell lines
via Sendai viral vectors encoding Yamanaka factors. Following transfection, pluripotent stem cell colonies
have emerged in the melanocyte cultures, as evident by phase-contrast and fluorescence microscopy images.
However, only cell clusters which were not positive for pluripotency markers were obtained in the melanoma
cell cultures. This proved that there are differences between healthy and cancer cell reprogramming. Basal
gene expression of Yamanaka factors, pluripotency markers and tumor-suppressor genes have been identified
in order to understand the different responses for cell reprogramming in melanocytes and cancer cell lines.
Future studies that will allow efficient reprogramming of cancer cells can improve our knowledge about cancer
biology and therapy

References

  • K. Takahashi, S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors, Cell, 126 (2006) 663-76.
  • I.H. Park, N. Arora, H. Huo, N. Maherali, T. Ahfeldt, A. Shimamura, M.W. Lensch, C. Cowan, K. Hochedlinger, G.Q. Daley, Disease-specific induced pluripotent stem cells, Cell, 134 (2008) 877-86.
  • F.T. Merkle, K. Eggan, Modeling human disease with pluripotent stem cells: from genome association to function, Cell. Stem Cell, 12 (2013) 656-68.
  • J. Sng, T. Lufkin, Emerging stem cell therapies: treatment, safety, and biology, Stem Cells Int., 2012 (2012) 521343.
  • M.A. Dawson, T. Kouzarides, Cancer epigenetics: from mechanism to therapy, Cell, 150 (2013) 12-27.
  • S.B. Baylin, J.E. Ohm, Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction?, Nat. Rev. Cancer, 6 (2006) 107-116.
  • A.P. Feinberg, B. Tycko, The history of cancer epigenetics, Nat. Rev. Cancer, 4 (2004) 143-153.
  • P.A. Jones, S.B. Baylin, The fundamental role of epigenetic events in cancer, Nat. Rev. Genet., 3 (2002) 415-428.
  • C.A. Gifford, Michael J. Ziller, H. Gu, C. Trapnell, J. Donaghey, A. Tsankov, A.K. Shalek, D.R. Kelley, A.A. Shishkin, R. Issner, X. Zhang, M. Coyne, J.L. Fostel, L. Holmes, J. Meldrim, M. Guttman, C. Epstein, H. Park, O. Kohlbacher, J. Rinn, A. Gnirke, E.S. Lander, B.E. Bernstein, A. Meissner, Transcriptional and epigenetic dynamics during specification of human embryonic stem cells, Cell, 153 (2013) 1149-1163.
  • W. Xie, M.D. Schultz, R. Lister, Z. Hou, N. Rajagopal, P. Ray, J. W. Whitaker, S. Tian, R.D. Hawkins, D. Leung, H. Yang, T. Wang, Ah Y. Lee, S.A. Swanson, J. Zhang, Y. Zhu, A. Kim, J.R. Nery, Mark A. Urich, S. Kuan, C.-a. Yen, S. Klugman, P. Yu, K. Suknuntha, N.E. Propson, H. Chen, L.E. Edsall, U. Wagner, Y. Li, Z. Ye, A. Kulkarni, Z. Xuan, W.-Y. Chung, N.C. Chi, J.E. AntosiewiczBourget, I. Slukvin, R. Stewart, M.Q. Zhang, W. Wang, J.A. Thomson, J.R. Ecker, B. Ren, Epigenomic analysis of multilineage differentiation of human embryonic stem cells, Cell, 153 (2013) 1134-1148.
  • J. Zhu, M. Adli, J.Y. Zou, G. Verstappen, M. Coyne, X. Zhang, T. Durham, M. Miri, V. Deshpande, P.L. De Jager, D.A. Bennett, J.A. Houmard, D.M. Muoio, T.T. Onder, R. Camahort, C.A. Cowan, A. Meissner, C.B. Epstein, N. Shoresh B.E. Bernstein, Genome-wide chromatin state transitions associated with developmental and environmental Cues, Cell, 152 (2013) 642-654.
  • B. Papp, K. Plath, Epigenetics of reprogramming to induced pluripotency, Cell, 152 (2013) 1324-43.
  • R.P. Koche, Z.D. Smith, M. Adli, H. Gu, M. Ku, A. Gnirke, B.E. Bernstein, A. Meissner, Reprogramming factor expression initiates widespread targeted chromatin remodeling, Cell. Stem. Cell, 8 (2013) 96-105.
  • A. Yilmazer, I. de Lázaro, H. Taheri, Reprogramming cancer cells: a novel approach for cancer therapy or a tool for disease-modeling?, Cancer Letters, 369 (2015) 1-8.
There are 14 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Açelya Yılmazer

Hadiseh Taheri This is me

Publication Date March 1, 2018
Acceptance Date October 12, 2017
Published in Issue Year 2018 Volume: 46 Issue: 1

Cite

APA Yılmazer, A., & Taheri, H. (2018). Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors. Hacettepe Journal of Biology and Chemistry, 46(1), 35-41.
AMA Yılmazer A, Taheri H. Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors. HJBC. March 2018;46(1):35-41.
Chicago Yılmazer, Açelya, and Hadiseh Taheri. “Reprogramming Human Melanocytes and Melanoma Cells With Yamanaka Factors”. Hacettepe Journal of Biology and Chemistry 46, no. 1 (March 2018): 35-41.
EndNote Yılmazer A, Taheri H (March 1, 2018) Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors. Hacettepe Journal of Biology and Chemistry 46 1 35–41.
IEEE A. Yılmazer and H. Taheri, “Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors”, HJBC, vol. 46, no. 1, pp. 35–41, 2018.
ISNAD Yılmazer, Açelya - Taheri, Hadiseh. “Reprogramming Human Melanocytes and Melanoma Cells With Yamanaka Factors”. Hacettepe Journal of Biology and Chemistry 46/1 (March 2018), 35-41.
JAMA Yılmazer A, Taheri H. Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors. HJBC. 2018;46:35–41.
MLA Yılmazer, Açelya and Hadiseh Taheri. “Reprogramming Human Melanocytes and Melanoma Cells With Yamanaka Factors”. Hacettepe Journal of Biology and Chemistry, vol. 46, no. 1, 2018, pp. 35-41.
Vancouver Yılmazer A, Taheri H. Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors. HJBC. 2018;46(1):35-41.

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