Research Article
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Year 2019, Volume: 47 Issue: 3, 267 - 275, 23.10.2019
https://doi.org/10.15671/hjbc.626949

Abstract

References

  • 1.J.S. Jurenka, Therapeutic applications of pomegranate (Punica granatum L.), Altern. Med. Rev., 13 (2008) 128-144.
  • 2. S. Medjakovic, A. Jungbauer, Pomegranate: a fruit that ameliorates metabolic syndrome, Food Funct., 4 (2013) 19- 39.
  • 3. E. Turrini, L. Ferruzzi, C. Fimognari, Potential effects of pomegranate polyphenols in cancer prevention and therapy, Oxid. Med. Cell Longevity, (2014) 1-20.
  • 4. G. Ma, C. Wang, L. Li, N. Ding, X. Gao, Effect of pomegranate peel polyphenols on human prostate cance PC-3 cells in vivo, Food Sci. Biotechnol., 24 (2015) 1887-1892.
  • 5. S. Banihani, S. Swedan, Z. Alguraan, Pomegranate and type 2 diabetes. Nutr. Res. 33 2013 341-348.
  • 6. S.R. Katz, R.A. Newman, E.P. Lansky, Punica granatum: heuristic treatment for diabetes mellitus, J. Med. Food., 10 (2007) 213–217.
  • 7. M.I. Gil, F.A. Tomas-Barberan, B. Hess-Pierce, D.M. Holcroft, A.A. Kader, Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing, J. Agric. Food Chem., 48 (2000) 4581.
  • 8. S.T. Lee, Y.L. Wu, L.H. Chien, S.T. Chen, Y.K. Tzeng, T.F. Wu, Proteomic exploration of the impacts of pomegranate fruit juice on the global gene expression of prostate cancer cell, Proteomics, 12 (2012) 3251-3262.
  • 9. V. Di Stefano, R. Pitonzo, M.E. Novara, D. Bongiorno, S. Indelicato, C. Gentile, G. Avellone, R. Bognanni, S. Scandurrac, M.G. Melillic, Antioxidant activity and phenolic composition in pomegranate (Punica granatum L.) genotypes from south Italy by UHPLC–Orbitrap-MS approach, 99 (2019) 1038- 1045.
  • 10. J. Lu, K. Ding, Q. Yuan, One-step purification of punicalagin by preparative hplc and stability study on punicalagin, Sep. Sci. Technol., 46 (2011) 147-154.
  • 11. M. Rosenblat, M. Aviram, N.P. Seeram, D. Heber, In vitro studies in pomegranates: ancient roots to modern medicine,Taylor and Francis Group, New York, 2006, 31-43.
  • 12. M. Aviram, L. Dornfeld, M. Rosenblat, N. Volkova, M. Kaplan, R. Coleman, T. Hayek, D. Presser, B. Fuhrman, Pomegranate Juice consumption reduces oxidative stress, atherogenic modification to ldl and platelet aggregation: studies in human and in atherosclerotic apolipoprotein deficient mice, Am. J. Clin.Nutr., 71 (2000) 1062-1076.
  • 13. E. Poyrazoglu, V. Gokmen, N. Artuk, Organic acids and phenolic compounds in pomegranates (Punica granatum) grown in Turkey, J. Food. Comp. Anal., 15 (2002) 567-575.
  • 14. S. Madrigal-Carballo, G. Rodriguez, C.G. Krueger, M. Dreher, J.D. Reed, Pomegranate (Punica granatum) supplements: Authenticity, antioxidant and polyphenol composition, J. Funct. Foods, 1 (2009) 324-329.
  • 15. P. Mena, L. Calani, C. Dall’Asta, G. Galaverna, C. GarcíaViguera, R. Bruni, A. Crozier, D. Del Rio, Rapid and comprehensive evaluation of (Poly) phenolic compounds in pomegranate (Punica granatum L.) juice by UHPLC–MS, Molecules, 17 (2012) 14821-14840.
  • 16. U.A. Fischer, R. Carle, D.R. Kammerer, Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSn, Food Chem., 127 (2011) 807-821.
  • 17. M. Wohig, A. Gledhill, J. Burgess, The determination of fruit juice authenticity using high resolution chromatography, uv, time‐of‐flight ms, and multivariate analysis [Online] Waters Corporation 2011.
  • 18. J. Lu, Y. Wei, Q. Yuan, Preparative separation of punicalagin from pomegranate husk by high-speed countercurrent chromatography, J. Chrom. B, 857 (2007) 175-179.
  • 19. J. Lu, K. Ding, Q. Yuan, Determination of punicalagin isomers in pomegranate husk, Chromatographia, 68 (2008) 303-306.
  • 20. H.V. Paithankar, HPLC method validations for pharmaceuticals: A review. IJUPBS. 2013 229-240.

Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS

Year 2019, Volume: 47 Issue: 3, 267 - 275, 23.10.2019
https://doi.org/10.15671/hjbc.626949

Abstract

Punica granatum L., commonly known as pomegranate, has a good source of bioactive polyphenolic compounds that
has been widely used as a traditional Chinese herbal medicine owing to their important biological properties including
cardiovascular disease, arthritis, diabetes, and cancer. Commercial pomegranate juice is generally obtained via pressing
whole pomegranate and its peels. Then, the most abundant ingredient, punicalagin, can be extracted into the juice. However, punicalagin levels may vary in commercial pomegranate juice and it can range from 0.017 to 2 g/L. In this study, we
analyzed punicalagin content from six different brands of pomegranate juices by using UPLC-MS method. First, punicalagin
standards were prepared from 50 µM to 5 µM in buffer A (0.1% formic acid in water) and standard calibration curve was
created. After that, three mass tune parameters including ionization voltage, desolvation temperature and source temperature of ESI source were optimized to obtain better peak shapes and correct quantification of the α- and β- isomer peaks of
punicalagin. After analysis of six different brands of pomegranate juices, it was seen that punicalagin levels from different
juice samples varied from 0.007 to 0.3 g/L.

References

  • 1.J.S. Jurenka, Therapeutic applications of pomegranate (Punica granatum L.), Altern. Med. Rev., 13 (2008) 128-144.
  • 2. S. Medjakovic, A. Jungbauer, Pomegranate: a fruit that ameliorates metabolic syndrome, Food Funct., 4 (2013) 19- 39.
  • 3. E. Turrini, L. Ferruzzi, C. Fimognari, Potential effects of pomegranate polyphenols in cancer prevention and therapy, Oxid. Med. Cell Longevity, (2014) 1-20.
  • 4. G. Ma, C. Wang, L. Li, N. Ding, X. Gao, Effect of pomegranate peel polyphenols on human prostate cance PC-3 cells in vivo, Food Sci. Biotechnol., 24 (2015) 1887-1892.
  • 5. S. Banihani, S. Swedan, Z. Alguraan, Pomegranate and type 2 diabetes. Nutr. Res. 33 2013 341-348.
  • 6. S.R. Katz, R.A. Newman, E.P. Lansky, Punica granatum: heuristic treatment for diabetes mellitus, J. Med. Food., 10 (2007) 213–217.
  • 7. M.I. Gil, F.A. Tomas-Barberan, B. Hess-Pierce, D.M. Holcroft, A.A. Kader, Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing, J. Agric. Food Chem., 48 (2000) 4581.
  • 8. S.T. Lee, Y.L. Wu, L.H. Chien, S.T. Chen, Y.K. Tzeng, T.F. Wu, Proteomic exploration of the impacts of pomegranate fruit juice on the global gene expression of prostate cancer cell, Proteomics, 12 (2012) 3251-3262.
  • 9. V. Di Stefano, R. Pitonzo, M.E. Novara, D. Bongiorno, S. Indelicato, C. Gentile, G. Avellone, R. Bognanni, S. Scandurrac, M.G. Melillic, Antioxidant activity and phenolic composition in pomegranate (Punica granatum L.) genotypes from south Italy by UHPLC–Orbitrap-MS approach, 99 (2019) 1038- 1045.
  • 10. J. Lu, K. Ding, Q. Yuan, One-step purification of punicalagin by preparative hplc and stability study on punicalagin, Sep. Sci. Technol., 46 (2011) 147-154.
  • 11. M. Rosenblat, M. Aviram, N.P. Seeram, D. Heber, In vitro studies in pomegranates: ancient roots to modern medicine,Taylor and Francis Group, New York, 2006, 31-43.
  • 12. M. Aviram, L. Dornfeld, M. Rosenblat, N. Volkova, M. Kaplan, R. Coleman, T. Hayek, D. Presser, B. Fuhrman, Pomegranate Juice consumption reduces oxidative stress, atherogenic modification to ldl and platelet aggregation: studies in human and in atherosclerotic apolipoprotein deficient mice, Am. J. Clin.Nutr., 71 (2000) 1062-1076.
  • 13. E. Poyrazoglu, V. Gokmen, N. Artuk, Organic acids and phenolic compounds in pomegranates (Punica granatum) grown in Turkey, J. Food. Comp. Anal., 15 (2002) 567-575.
  • 14. S. Madrigal-Carballo, G. Rodriguez, C.G. Krueger, M. Dreher, J.D. Reed, Pomegranate (Punica granatum) supplements: Authenticity, antioxidant and polyphenol composition, J. Funct. Foods, 1 (2009) 324-329.
  • 15. P. Mena, L. Calani, C. Dall’Asta, G. Galaverna, C. GarcíaViguera, R. Bruni, A. Crozier, D. Del Rio, Rapid and comprehensive evaluation of (Poly) phenolic compounds in pomegranate (Punica granatum L.) juice by UHPLC–MS, Molecules, 17 (2012) 14821-14840.
  • 16. U.A. Fischer, R. Carle, D.R. Kammerer, Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSn, Food Chem., 127 (2011) 807-821.
  • 17. M. Wohig, A. Gledhill, J. Burgess, The determination of fruit juice authenticity using high resolution chromatography, uv, time‐of‐flight ms, and multivariate analysis [Online] Waters Corporation 2011.
  • 18. J. Lu, Y. Wei, Q. Yuan, Preparative separation of punicalagin from pomegranate husk by high-speed countercurrent chromatography, J. Chrom. B, 857 (2007) 175-179.
  • 19. J. Lu, K. Ding, Q. Yuan, Determination of punicalagin isomers in pomegranate husk, Chromatographia, 68 (2008) 303-306.
  • 20. H.V. Paithankar, HPLC method validations for pharmaceuticals: A review. IJUPBS. 2013 229-240.
There are 20 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Kemal Solakyıldırım

Publication Date October 23, 2019
Acceptance Date May 28, 2019
Published in Issue Year 2019 Volume: 47 Issue: 3

Cite

APA Solakyıldırım, K. (2019). Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS. Hacettepe Journal of Biology and Chemistry, 47(3), 267-275. https://doi.org/10.15671/hjbc.626949
AMA Solakyıldırım K. Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS. HJBC. October 2019;47(3):267-275. doi:10.15671/hjbc.626949
Chicago Solakyıldırım, Kemal. “Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica Granatum L.) Juices by UPLC-MS”. Hacettepe Journal of Biology and Chemistry 47, no. 3 (October 2019): 267-75. https://doi.org/10.15671/hjbc.626949.
EndNote Solakyıldırım K (October 1, 2019) Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS. Hacettepe Journal of Biology and Chemistry 47 3 267–275.
IEEE K. Solakyıldırım, “Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS”, HJBC, vol. 47, no. 3, pp. 267–275, 2019, doi: 10.15671/hjbc.626949.
ISNAD Solakyıldırım, Kemal. “Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica Granatum L.) Juices by UPLC-MS”. Hacettepe Journal of Biology and Chemistry 47/3 (October 2019), 267-275. https://doi.org/10.15671/hjbc.626949.
JAMA Solakyıldırım K. Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS. HJBC. 2019;47:267–275.
MLA Solakyıldırım, Kemal. “Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica Granatum L.) Juices by UPLC-MS”. Hacettepe Journal of Biology and Chemistry, vol. 47, no. 3, 2019, pp. 267-75, doi:10.15671/hjbc.626949.
Vancouver Solakyıldırım K. Fast Punicalagin Content Analysis of Various Brands of Pomegranate (Punica granatum L.) Juices by UPLC-MS. HJBC. 2019;47(3):267-75.

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