Exosome Production, Isolation and Characterization from A549 Epithelial Carcinoma Cells

Year 2019, Volume: 47 Issue: 4, 383 - 388, 01.12.2019

Esra Mutlu , Özge Kaya Arzu Birinci Yildirim , Ayhan Çetinkaya

https://doi.org/10.15671/hjbc.520101

Cited By: 3

Abstract

Exosomes
are natural nanoparticles that special features have been started to be used
for recent years in the treatment of cancer as a natural, homogeneous,
nanosized, targeted vesicles (~ 50- 100 nm). They have high avidity (many
conformational attachment) to attach onto targeted cancer cell surfaces. They
are composed of bioactive double-layered lipid layers in which their original
nature has the adhesive proteins interacting with the cancer cell membrane
easily. In this study, the exosomes of non-small cell lung cancer A549
epithelial carcinoma cells were investigated for their potential to be the
natural or synthetic drug carrier. Firstly, exosomes of A549 cell line were
produced in exosome-free media. Immediately after, isolation of their exosomes
were realized by using ultracentrifugation procedure. SEM image, particle size,
zeta potential, exosomal RNA analysis and Protein Content by Bradford assays
were performed. Findings (Size: 168 nm; zeta: -16mV) on the properties of A549
cell exosomes proved their potential to be used as the drug carrier for cancer
cell therapy.

Keywords

Exosome, ultracentrifugation, A549 Cell Line, miRNA

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