Improved Morphology and Biocompatibility of Chitosan/PEO Casting Films Adding Hyaluronic Acid

Year 2018, Volume: 46 Issue: 3, 365 - 372, 01.09.2018

Esra Cansever Mutlu Muhammet Yıldırım

Abstract

Biopolymer-based blends can be used within the form of cast film in various proportions with polyethylene
oxide (PEO) in order to obtain more durable and biocompatible surfaces. Chitosan and hyaluronic acid (HA)
are the most used biocompatible biopolymers for the production of films. On the other hand, as a synthetic
polymer, PEO has been mostly used to improve durability of the surfaces by using in various molecular weights.
This study was carried out to determine the effect of HA on the chitosan and PEO biopolymer blends different
from reported Chitosan/PEO film surfaces in the literature. FTIR studies presented no any polycationic amine
groups belong to chitosan through the surface of four films in different compositions after casting. Surface
morphology were determined by SEM, EDS and polarized microscopy analyses. Surface morphology was observed
as well-oriented spherulitic crystallization by the increasing amount of PEO in the films. Astonishingly,
it was proven that HA improved the biocompatibility feature of PEO-rich surfaces which were tested by using
healthy mouse fibroblast cell lines, L929 (ATCC CCL-1).

Keywords

Biopolymer blends, chitosan, PEO, hyaluronic acid, film casting

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