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Year 2020, Volume 48 , Issue 4, Pages 333 - 339 2020-07-06

DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits

Zübeyde UĞURLU AYDIN [1] , Mustafa K. ŞENOVA [2] , Marcus KOCH [3] , Ali DÖNMEZ [4]

Abstract

GeneAll GenExTM Plant kiti, GeneMATRIX Plant & Fungi DNA Purification kiti, DNeasy Plant kiti ve E.Z.N.A. Plant DNA kiti yaprak ve gövde dokusu DNA barkodlama çalışmaları için kaliteli ve yüksek genomik DNA elde etmek amacıyla test edilmiştir. Geleneksel olarak yaprak dokusu genomik DNA kaynağı olarak kullanılmaktadır fakat Dianthus taksonları yaprak sklerankima dokusu nedeniyle bu bakımdan sorunludur. Genomik DNA, üretici firmanın sağladığı protokole göre elde edilmiştir ve yedi barkod bölgesi PCR yöntemiyle çoğaltılmıştır. DNA konsantrasyonu florometrik yöntemle ölçülmüş, PCR ürünleri agaroz jelde görüntülenmiştir. Sonuçlar, DNA barkodlama çalışmalarında kullanılabilecek PCR başarısı bakımından değerlendirilmiştir. Ayrıca, kullanılan ekstraksiyon kitleri zahmet, fiyat ve zaman bakımından değerlendirilmiştir. Sonuçlarımıza göre, yaprak ve gövde dokusundan genomik DNA ekstraksiyonu başarıyla gerçekleştirilmiştir. Tüm ekstraksiyon kitleri PCR amplifikasyonu bakımından başarılı olmasına rağmen en yüksek verim GeneMATRIX kiti kullanılarak elde edilmiştir.

Keywords

Dianthus, DNA barcoding, DNA isolation, PCR success, stem

References

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Details

Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-1113-3074
Author: Zübeyde UĞURLU AYDIN (Primary Author)
Institution: HACETTEPE UNIVERSITY
Country: Turkey

Orcid: 0000-0001-9574-7876
Author: Mustafa K. ŞENOVA
Institution: HACETTEPE UNIVERSITY
Country: Turkey

Orcid: 0000-0002-1693-6829
Author: Marcus KOCH
Institution: University of Heidelberg
Country: Germany

Orcid: 0000-0001-6695-6177
Author: Ali DÖNMEZ
Institution: HACETTEPE UNIVERSITY
Country: Turkey
Thanks The study was conducted as a part of the project titled “DNA Barcoding Research on some Dianthus L. (Caryophyllaceae) in Turkey” supported by Hacettepe University (FBA-2018-16187).
Dates

Publication Date : July 6, 2020

Cite

Bibtex @research article { hjbc663505, journal = {Hacettepe Journal of Biology and Chemistry}, issn = {2687-475X}, eissn = {2687-475X}, address = {Hacettepe Üniversitesi Fen Fakültesi, 06532, Beytepe/ ANKARA/ TÜRKİYE}, publisher = {Hacettepe University}, year = {2020}, volume = {48}, pages = {333 - 339}, doi = {10.15671/hjbc.663505}, title = {DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits}, key = {cite}, author = {Uğurlu Aydın, Zübeyde and Şenova, Mustafa K. and Koch, Marcus and Dönmez, Ali} }
APA Uğurlu Aydın, Z , Şenova, M , Koch, M , Dönmez, A . (2020). DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits . Hacettepe Journal of Biology and Chemistry , 48 (4) , 333-339 . DOI: 10.15671/hjbc.663505
MLA Uğurlu Aydın, Z , Şenova, M , Koch, M , Dönmez, A . "DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits" . Hacettepe Journal of Biology and Chemistry 48 (2020 ): 333-339 <https://dergipark.org.tr/en/pub/hjbc/issue/55838/663505>
Chicago Uğurlu Aydın, Z , Şenova, M , Koch, M , Dönmez, A . "DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits". Hacettepe Journal of Biology and Chemistry 48 (2020 ): 333-339
RIS TY - JOUR T1 - DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits AU - Zübeyde Uğurlu Aydın , Mustafa K. Şenova , Marcus Koch , Ali Dönmez Y1 - 2020 PY - 2020 N1 - doi: 10.15671/hjbc.663505 DO - 10.15671/hjbc.663505 T2 - Hacettepe Journal of Biology and Chemistry JF - Journal JO - JOR SP - 333 EP - 339 VL - 48 IS - 4 SN - 2687-475X-2687-475X M3 - doi: 10.15671/hjbc.663505 UR - https://doi.org/10.15671/hjbc.663505 Y2 - 2020 ER -
EndNote %0 Hacettepe Journal of Biology and Chemistry DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits %A Zübeyde Uğurlu Aydın , Mustafa K. Şenova , Marcus Koch , Ali Dönmez %T DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits %D 2020 %J Hacettepe Journal of Biology and Chemistry %P 2687-475X-2687-475X %V 48 %N 4 %R doi: 10.15671/hjbc.663505 %U 10.15671/hjbc.663505
ISNAD Uğurlu Aydın, Zübeyde , Şenova, Mustafa K. , Koch, Marcus , Dönmez, Ali . "DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits". Hacettepe Journal of Biology and Chemistry 48 / 4 (July 2020): 333-339 . https://doi.org/10.15671/hjbc.663505
AMA Uğurlu Aydın Z , Şenova M , Koch M , Dönmez A . DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits. HJBC. 2020; 48(4): 333-339.
Vancouver Uğurlu Aydın Z , Şenova M , Koch M , Dönmez A . DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits. Hacettepe Journal of Biology and Chemistry. 2020; 48(4): 333-339.
IEEE Z. Uğurlu Aydın , M. Şenova , M. Koch and A. Dönmez , "DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits", Hacettepe Journal of Biology and Chemistry, vol. 48, no. 4, pp. 333-339, Jul. 2020, doi:10.15671/hjbc.663505
Authors of the Article
Zübeyde UĞURLU AYDIN [1]
Mustafa K. ŞENOVA [2]
Marcus KOCH [3]
Ali DÖNMEZ [4]
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