In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis

Year 2007, Volume: 35 Issue: 2, 97 - 100, 01.04.2007

Dudu Özkum

Abstract

In vitro shoot regeneration procedure for oregano O. minutiflorum O. Schwarz & Davis , endemic species of Turkey,is described. Leaf segments and shoot explants hypocotyl, single nodal segment and shoot tip excised from 30-40d old in vitro germinated seedlings and cultured on Murashige and Skoog MS and Gamborg B5 media containingdifferent combinations of benzyl amino purine BAP 0.0, 1.0, 2.0 or 3.0 mg L-1 and naphtalene acetic acid NAA 0.0, 0.1 or 0.5 mg L-1 . The single nodal segments were the most succesful explant in all hormone combinationsused. MS medium supplemented with 2.0 mg. L-1BAP + 0.1 mg L-1NAA was the most effective medium for shootformation. However for all explants, B5 medium with all phytohormone combinations had no effective success andtherefore were not suitable on in vitro shoot regeneration

Keywords

Origanum minutiflorum, shoot regeneration, In vitro, micropropagation

References

• 1. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plantarum, 15, 473- 497, 1962.
• 2. Gamborg, O.L., Miller, R.A. and Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res., 50, 151- 158, 1968.
• 3. Bajaj, Y.P.S, Furmanowa, M. and Olszowska, O. Biotechnology of the micropropagation of medicinal and aromatic plants. p. 60-103. In: Y.P.S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry 4. Medicinal and Aromatic Plants I. Springer-Verlag, Berlin, 1988.
• 4. Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. and Adıgüzel, N. Türkiye Bitkileri Kırmızı Kitabı. Türkiye Tabiatını Koruma Derneği, VanYüzüncü Yıl Üniv. Ankara, 2000.
• 5. IUCN. Red List Categories: Version 3.1. Prepared By IUCN Species Survival Commission, IUCN, Gland Switzerland and Cambridge, UK, 2001.
• 6. Kumari, N. and Saradhi, P.P. Regeneration of plants from callus cultures of Origanum vulgare L. Plant Cell Rep., 11 (9), 476-479, 1992.
• 7. Crutis, O.F. and Shetty, K. Growth medium effects of vitrification, total phenolics, chlorophyll and water content of in vitro propagated oregano clones. Acta Hort., 426, 489-497, 1996.
• 8. Iyer, P.V. and Pai, J.S. In vitro regeneration of Majorana hortensis Moench from callus and nodal stem segments. Journal of Species and Aromatic Crops, 9 (1), 47-50, 2000.
• 9. Tisserat, B. and Vaughn, S.F. Techniques to improve growth, morphogenesis and secondary metabolism responses from Lamiaceae species in vitro. Proc. XXVI IHC-Future for Medicinal and Aromatic Plants Eds. L.E.Craker et al., Acta Hort. 629, ISHS, 333-339, 2004.
• 10. Minas, G.J. Certain dittany apical meristem micropropagation in vitro, Miscellaneous-Reports Agricultural Research Institute Ministry of Agriculture, Natural Resources and the Environment, No. 80, 7pp, 2001.
• 11. Davis, P.H., Mill, R.R. and Tan, K. (Ed.). Flora of Turkey and the East Aegean Islands, Edinburgh Univ. Press., Edinburgh. Vol. 7, pp. 186- 187 and pp. 307-308, 1982.
• 12. Kitiki, A. Status of Cultivation and Use of Oregano in Turkey , Proceedings of the IPGRI International Workshop on Oregano, CIHEAM, Valenzano (Bari), 122-132, 1996.
• 13. Duman, H. Origanum L. in: Güner, A., Özhatay, N., Ekim, T., Başer, K.H.K., (Ed.). Flora of Turkey and East Aegean Islands, Edinburg University Press, Edinburg, Vol 11, pp. 207- 208, 2000.
• 14. Baytop, A. Farmasötik Botanik, İstanbul Üniv. Eczacılık Fak. Yayınları, 36, 282-285, 1983.
• 15. Lagouri, V., Blekas, G., Tsimidou, M, Kokkini, S. and Boskou, D. Composition and antioxidant activity of essential oils from oregano plants grown wild in Greece. Z. Lebensm. Unters. Frosch,. 197: 20-23, 1993.
• 16. Sivropoulou, A., Papanicolaou, E., Nikolaou, C., Kokkoni, S., Lanaras, T. and Arsenakis, M. Antimicrobial and cytotoxic activities of Origanum essential oils. J. Agric. Food Chem. 44: 1202-1205, 1996.
• 17. Sokal, R. and Rohlf, F.J. Biometry, The Principles and Practice of Statistics in Biological Research, Third Edition, W.H. Freeman and Co., New York, USA, 887 p, 1995.
• 18. Ćellárová, E. Micropropagation Mentha L., Biotechnology in Agriculture And Forestry 19, High Tech. and Micropropagation II, Bajaj, Y.P.S. (eds.) Springer-Verlag, pp. 262-276, 1992.
• 19. Faria J.L.C., Kostenyuk, I. and Segura, J. Isolation, culture and plant regeneration from protoplasts of Sideritis angustifolia. J. of Plant Physiol., 153 (1-2), 251-254, 1998.
• 20. Tepe, Ş., Ellialtıoğlu, Ş., Yenice, N. and Tıpırdamaz, R. In vitro kolhisin uygulaması ve poliploid nane (Mentha longifolia L.) bitkilerinin elde edilmesi, Akdeniz Üniversitesi Ziraat Fakültesi Dergisi., 15 (2), 63-69, 2002.
• 21. Ayan, A.K., Çırak, C., Kevseroğlu, K. and Sökmen, A. Effects of explant types and different concentrations of sucrose and phytohormones on plant regeneration and hypericin content in Hypericum perforata L., Tr. J. Agr. For., 29, 197-204, 2005.
• 22. Zobayed, S.M.A. and Saxena, P.K. In vitrogrown roots: a superior exsplant for perlific shoot regeneration of St. John’s wort (Hypericum perforatum L. cv “New Stem”) in a temporary immersion bioreactor. Plant. Sci., 165, 463-470, 2003.
• 23. Iyer, P.V. and Pai, J.S. Micropropagation of sweet marjoram (Majorana hortensis Moech.), Journal of Species and Aromatic Crops, 7 (1), 47-79, 1998.
• 24. Gupta, S.D. and Conger, B.V. Differentiation of multiple shoot clumps from intact seedling of switchgrass. In Vitro Cell. and Develop., 34, 196-202, 1998.
• 25. Werbrouck, S.P.O. and Debergh, P.C. Applied aspects of plant regeneration (micropropagation). Plant Cell Culture – A Pratical Approach, Dixon, R.A and Gonzales, R.A. (Ed.) Oxford Uni. Press., New York, pp. 127-135, 1994.