Poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel was preparedby bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column cryo-polymerization . After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected poresof 10-50 μm size. Then the monolithic cryogel was loaded with Fe III ions to form the metal chelate with poly HEMAMAH cryogel. Poly HEMA-MAH cryogel was characterized by swelling studies, FTIR, scanning electron microscopy,and elemental analysis. The equilibrium swelling degree of the poly HEMA-MAH monolithic cryogel was 2.82 g H2O/gcryogel. Poly HEMA-MAH cryogel containing 36.4 μmol MAH/g was used in the adsorption/desorption of lysozymefrom aqueous solutions. The nonspecific adsorption of lysozyme was very low 1.1 mg/g . The maximum amount oflysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity
Ergün, B., Derazshamshir, A., & Odabaşı, M. (2007). Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. Hacettepe Journal of Biology and Chemistry, 35(2), 143-148.
AMA
Ergün B, Derazshamshir A, Odabaşı M. Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. HJBC. April 2007;35(2):143-148.
Chicago
Ergün, Bahar, Ali Derazshamshir, and Mehmet Odabaşı. “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”. Hacettepe Journal of Biology and Chemistry 35, no. 2 (April 2007): 143-48.
EndNote
Ergün B, Derazshamshir A, Odabaşı M (April 1, 2007) Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. Hacettepe Journal of Biology and Chemistry 35 2 143–148.
IEEE
B. Ergün, A. Derazshamshir, and M. Odabaşı, “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”, HJBC, vol. 35, no. 2, pp. 143–148, 2007.
ISNAD
Ergün, Bahar et al. “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”. Hacettepe Journal of Biology and Chemistry 35/2 (April 2007), 143-148.
JAMA
Ergün B, Derazshamshir A, Odabaşı M. Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. HJBC. 2007;35:143–148.
MLA
Ergün, Bahar et al. “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 2, 2007, pp. 143-8.
Vancouver
Ergün B, Derazshamshir A, Odabaşı M. Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. HJBC. 2007;35(2):143-8.