Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads

Bilgen Osman; Ali Kara; Necati Beşirli

Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads

Year 2007, Volume: 35 Issue: 3, 233 - 241, 01.08.2007

Abstract

Poly ethylene glycol dimethacrylate-n-vinyl imidazole [poly EGDMA-VIM ] hydrogel beads average diameter 150–200 µm was prepared by copolymerizing of ethylene glycol dimethacrylate EGDMA with n-vinyl imidazole VIM .Poly EGDMA-VIM beads had a specific surface area of 59.8 m2/g. Poly- EGDMA-VIM beads were characterized byswelling studies, elemental and SEM. Cu2+ions were chelated on the poly EGDMA-VIM beads, then these beadswere used in the reversible adsorption of mushroom tyrosinase. The maximum tyrosinase adsorption capacity of thepoly EGDMA-VIM –Cu2+beads was observed as 14.04 mg/g at pH 6.5. Storage stability was found to increase withimmobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss inadsorption capacity or enzyme activity

Keywords

Enzyme immobilization, Tyrosinase, Chelating beads, n-Vinyl imidazole, Affinity beads

References

S. Wada S. Ichikawa, K. Tatsumi, Biotechnol. Bioeng., 45 (1995) 304. 11. S. Davis, R.G. Burs, Appl Microbial Biotechnol., 32 (1990) 721.
R.S. Freire, N. Duran, L.T. Kubota, Talanta, 54 (2001) 681.
A.I. Yaropolov, A.N. Kharybin, J. Emneus, G. Marko- Varga, Anal Chim Acta, 308 (1995) 137.
A.V. Vakurov, A.K. Gladilin, A.V. Levashov, Y.L. Khmelnitsky, Biotechnol Lett., 16 (1994) 175.
C. Crecchio, P. Ruggiero, M.D.R. Pizzigallo, M. Curci, Dev Plant Soil Sci., 71 (1997) 545.
G. DeStefano, P. Piacquadio, V. Sciancalepore, Biotechnol. Technol., 10 (1996) 857.
A. Kara, L. Uzun, N. Besirli, A. Denizli, J. Hazard. Mater., 106 (2004) 93.
A. Kara, B. Osman, H. Yavuz, A. Denizli, React. Functl. Polym., 62 (2005) 61.
J. Rodakiewicz-Nowak, M. Monkiewicz, J. Haber, Colloids Surf. A 208 (2002) 347.
N. Duran, M.A. Rosa, A. D’Annibale, L. Gianfreda, Enzyme Microb.Technol., 31 (2002) 907.
A. Denizli, M. Alkan, B. Garipcan, S. Özkara, E. Pişkin, J. Chromatogr. B, 795 (2003) 93.
Y.H. Tsai, M.Y. Wang, S.Y. Suen, J. Chromatogr. A, 766 (2001) 133.
S. Emir, R. Say, H. Yavuz, A. Denizli, Biotechnol. Prog., 20 (2004) 223.

There are 13 citations in total.

Details

Primary Language	English
Journal Section	Research Article
Authors	Bilgen Osman This is me Ali Kara This is me Necati Beşirli This is me
Publication Date	August 1, 2007
Published in Issue	Year 2007 Volume: 35 Issue: 3

Cite

APA	Osman, B., Kara, A., & Beşirli, N. (2007). Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. Hacettepe Journal of Biology and Chemistry, 35(3), 233-241.
AMA	Osman B, Kara A, Beşirli N. Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. HJBC. August 2007;35(3):233-241.
Chicago	Osman, Bilgen, Ali Kara, and Necati Beşirli. “Tyrosinase Immobilization on Cu2+Chelated Poly Ethylene Glycol Dimethacrylate-N-Vinyl Imidazole Beads”. Hacettepe Journal of Biology and Chemistry 35, no. 3 (August 2007): 233-41.
EndNote	Osman B, Kara A, Beşirli N (August 1, 2007) Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. Hacettepe Journal of Biology and Chemistry 35 3 233–241.
IEEE	B. Osman, A. Kara, and N. Beşirli, “Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads”, HJBC, vol. 35, no. 3, pp. 233–241, 2007.
ISNAD	Osman, Bilgen et al. “Tyrosinase Immobilization on Cu2+Chelated Poly Ethylene Glycol Dimethacrylate-N-Vinyl Imidazole Beads”. Hacettepe Journal of Biology and Chemistry 35/3 (August 2007), 233-241.
JAMA	Osman B, Kara A, Beşirli N. Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. HJBC. 2007;35:233–241.
MLA	Osman, Bilgen et al. “Tyrosinase Immobilization on Cu2+Chelated Poly Ethylene Glycol Dimethacrylate-N-Vinyl Imidazole Beads”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 3, 2007, pp. 233-41.
Vancouver	Osman B, Kara A, Beşirli N. Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. HJBC. 2007;35(3):233-41.