Boronic Acid Functionalized Adsorbents for Antibody Removal

Year 2008, Volume: 36 Issue: 1, 47 - 60, 01.01.2008

Başak Şaşmaz Gözde Baydemir Nilay Bereli Serap Şenel Handan Yavuz

Abstract

In this study a novel approach was developed to obtain an efficient separation of human-IgG from human plasma. 4- Vinylphenylboronic acid VPBA and 2-hydroxyethylmethacrylate HEMA were chosen as monomers. Spherical microbeads with an average size of 100-140 µm were obtained by the radical suspension polymerization of VPBA and HEMA conducted in an aqueous dispersion medium. VPBA monomer was characterized by FTIR. p HEMA-VPBA beads were characterized by swelling studies, FTIR and SEM. The pHEMA and p HEMA-VPBA microbeads were contacted with blood in in-vitro systems. Loss of the blood cells and the clotting times were also followed. Good blood compatibility properties of both pHEMA and p HEMA-VPBA were clearly observed with blood coagulation experiments. Loss of cells in the blood contacting with pHEMA and p HEMA-VPBA microbeads were nearly the same. These VPBA containing p HEMA-VPBA microbeads with a swelling ratio of 57 % were used in the adsorption/desorption of human-IgG from aqueous solutions and human plasma. The maximum human-IgG adsorption 139 mg/g polymer was observed at pH 8.5 for HEPES buffer. IgG adsorption from human plasma was 38.75 mg/g. It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.

Keywords

Boronic Acid, Antibody Removal, Boranate affinity

References

• 1. Huse K., Böhme H.J., Scholz G.H., J. Biochem Biophys. Methods, 2002, 51, 217.
• 2. Goding, J.W., Monoclonal Antibodies: Principles and Practice, New York 1995.
• 3. Serpaa, G., Augusto, E.F.P., Tamashiroc, W., Riberiora, M.B., Mirandaa, E.A., Buenoa, S.M.A., Journal of Chromatography B, , 2005, 816, 259.
• 4. Riske, F., Smith, M., Menon, M., Goetschalck, S., Goidsenhoven, I.V., Krul, A., Pimpaneau, V., Renaers, I., Van Tichelt, N., Van Houdt, K., Hayes, M., Lawrence, C., Bigelow, R., Schroeder, J., Journal of Chromatography B, 2007, 848, 108.
• 5. Ishihara, T., Kadoya, T., Journal of Chromatography A, , 2007, 1176, 149.
• 6. Galina A.P., Tatiana B.T., Journal of Chromatography A, , 2005, 1065, 19.
• 7. Zimmerman, T., Frère, C.P., Satzger, M., Raba, M., Weisbach, M., Journal of Immunological Methods , 2006, 314, 67.
• 8. Roque, A.C.A., Silva, C.S.O., Taipa, M.A., Döhn, K., Popp, A., Donzeau, M., Journal of Chromatography A, , 2007, 1160, 44.
• 9. Denizli, A., Arıca, M.Y., J. Biomater. Sci. Polym. Ed. 11, , 2000, 367.
• 10. Hober, S., Nord, K., Linhult, M., Journal of Chromatography B, , 2007, 848, 40.
• 11. McCue, J.T., Kemp, G., Low, D., Quinones-Garcia, I., J. Chromatogr. A , 2003, 989, 139.
• 12. Jungbauer, A., Hahn, R., Curr. Opion. Drug Dis. Dev. 7, 2004, 248.
• 13. Hahn, R., Schlegel, R., Jungbauer, A., J. Chromatogr. B , 2003, 790, 25.
• 14. Reis, K.J., Boyle, M.D.P., Ayoub, E.M., J. Clin. Lab. Immunol. , 1984, 13, 75.
• 15. Fahrner, R.L., Whitney, D.H., Vanderlaan, M., Blank, G.S., Biotechnol. Appl. Biochem., 1999, 30, 121.
• 16. Hahn, R., Shimahara, K., Steindl, F., Jungbauer, A., J. Chromatogr. A,, 2006, 1102, 224.
• 17. Bak, H., Thomas, O.R.T, Journal of Biotechnology, 2007, 131S, S130.
• 18. Zhou, J.X. Dermawan, S., Solamo, F., Flynn, G., Stenson, R., Tressel, T., Guhan, S., Journal of Chromatography A, , 2007, 1175, 69.
• 19. Rena, D., Pipes, G.D., Hambly, D.M., Bondarenko, P.V., Treuheit, M.J., Bremsa, D.N., Gadgil, H.S., Journal of Chromatography A, 2007, 1175, 63.
• 20. Temperature-Triggered Purification of Antibodies, Kim, J.Y., Mulchandani, A., Chen, W., Biotechnol. Bioeng., 2005, Vol. 90, No. 3, May 5, 373.
• 21. Roque, A.C., Taipa, M.A., Lowe, C.R., Journal of Chromatography A,, 2005, 1064, 157.
• 22. Roque, A.C., Taipa, M.A., Lowe, C.R., J. Mol. Recognit., 2004, 262.
• 23. Verdoliva, A., Marasco, D., De Capua, A., Saporito, A., Bellofiore, P., Manfredi, V., Fattorusso, R., Pedone, C., Ruvo, M., ChemBioChem, 2005, 6, 1242.
• 24. Özkara, S., Yavuz, H., Patır, S., Arıca, M.Y., Denizli, A., Separation Science and Technology, 2002, 37, 717.
• 25. Coffinier, Y., Legallais, C., Vijayalakshmi, M.A., J. Membr. Sci., 2002, 208, 13.
• 26. Yang, L., Chen, P., J. Membr. Sci., 2002, 205, 141.
• 27. Kalaycioğlu, E., Patir, S., Piskin, E., Langmuir, 2003, 19, 9538.
• 28. Bueno, S.M.A., Haupt, K., Vijayalakshmi, M.A., J. Chromatogr. B, 1995, 667, 57.
• 29. Özkara, S., Akgöl, S., Çanak, Y., Denizli, A., Biotechnol. Prog. 20, 2004, 1169.
• 30. Çanak, Y., Özkara, S., Akgöl, S., Denizli, A., React. Funct. Polym., 2004, 61, 369.
• 31. Xiao-Chuan, L., Chin J Chromatogr, , 2006, 24 (1) 73.
• 32. Weith, H.L, Wiebers, J.L., Gilham, P.T., Biochemistry, 1970, 9, 4396.
• 33. Singh, N., Willson, R.C., Journal of Chromatography A, , 1999, 840, 205.
• 35. Elmas, B., Onur, M.A., Senel, S., Tuncel, A., Colloids and Surfaces A: Physicochem. Eng. Aspects , 2004, 232, 253.
• 36. Özdemir, A., Tuncel, A., Journal of Applied Polymer Science, Vol. , 2000, 78, 268.
• 37. Lei, Y., Liu, Z., Liu, Q., Wu, X., Reactive & Functional Polymers, 2001, 48, 159.
• 38. Ivanov, A.E., Galaev, I.Y., Mattiasson, B., J. Mol. Recognit. , 2006, 19, 322.
• 39. Palanisamy, U.D., Hussain, A., Iqbal, S., Sproule, K., Lowe, C.R., J. Mol. Recognit., , 1999, 12, 57.
• 40. Li, Y., Jeppsson, J.O., Karlsson, M.J., Larsson, E.L., Jungvida, H., Galaev, I.Y., Mattiasson, B., Journal of Chromatography B, , 2002, 776, 149.
• 41. Tanaka, T., Matsunaga, T., Biosens. Bioelectron., 2001, 16, 1089.
• 42. Cannizzo, C., Gerbier, A.S., Larpent, C., Polymer, 2005, 46, 1269.
• 43. Kanayama, N., Kitano, H., Langmuir, , 2000, 16, 577.
• 44. Sanz, V.C., Mena, M.L., Cortes, A.G., Sedeno, P.Y., Pingarron, J.M., Analytica Chimica Acta , 2005, 528, 1.
• 45. Matsumoto, A., Ikeda, S., Harada, A., Kataoka, H., Biomacromolecules, 2003, 4, 1410.
• 46. Denizli, A., Pişkin, E., J. Chromatogr. B,, 1995, 666, 215.
• 47. Denizli, A., Pişkin, E., J. Chromatogr. B, , 1995, 670, 157.
• 48. Lagergren, H., Olsson, P., Swedenborg, J., Surgery,, 1974, 75, 643.
• 49. Doumas, B.R., Biggs, H., Standart methods of Clin. Chem., , 1972, Vol 7.
• 50. Peppas, N., Hydrogels, in Biomaterials Science, Hoffmann, A. Ed., Academic Pres, New York, 1996, 60-64.
• 51. Denizli, A., J. Appl. Polym. Sci., , 1999, 74, 655.
• 52. Brash, J.L., Modern Aspects of Protein Adsorption on Biomaterials, Missirlis, Y.F., Lemm, W. Eds., Kluwer Academic Publishers, 1991, 39-47.
• 53. Haupt, K., Bueno, S.M.A. and Vijayalakshmi, M.A., Journal of Chromatography B,, 1995, 674, 13.