Magnetic Affinity Separation of Recombinant Fusion Proteins

Year 2010, Volume: 38 Issue: 1, 1 - 7, 01.01.2010

Ivo Safarik Mirka Safarikova

Abstract

Isolation and separation of proteins is an important procedure in all areas of biosciences and biotechnology. Currently recombinant fusion proteins are produced routinely; in order to simplify their isolation from complex samples, magnetic separation techniques can be efficiently used. This short review paper shows examples of the most important fusion affinity tags and commercially available magnetic affinity and ion exchange particles enabling efficient capture of recombinant fusion proteins. A list of realized isolation procedures documents the efficiency of magnetic separation techniques.

Keywords

Recombinant fusion proteins, Affinity tags, Magnetic separation, Magnetic adsorbents

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