Measurement of Melatonin in Pearl mullets, an Endemic Species Growing in Lake Van, and Trouts

Year 2011, Volume: 39 Issue: 4, 421 - 426, 01.12.2011

Tülay Gürsoy Nurhayat Atasoy İhsan Alacabey

Abstract

In the present study, the melatonin levels were measured in pearl mullets also known as Van fish and trouts. Pearl mullets grow naturally in Lake Van in Turkey, and trouts, raised by private fish farms, were used as study materials. These fish are known to secrete melatonin, a hormone associated with development and stress, as occurs in human beings. Melatonin was measured in the plasma taken from living pearl mullets and trouts by an ELISA method using commercial kits. The study was conducted in accordance with the ELISA kit procedure. In the spring, the mean melatonin levels of trouts were 160.8 ± 4.090 pg/mL in light and 198.3 ± 3.694 pg/mL in dark, whereas in winter 86.69 ± 3.203 pg/mL and 128.9 ± 1.999 pg/mL in light and dark, respectively. In pearl mullets, the mean melatonin levels were 84.73 ± 2.281 pg/mL in light and 118.1 ± 2.560 pg/mL in dark in spring, and in winter 36.32 ± 1.627 pg/mL in light and 70.53 ± 2.919 pg/mL in darks p < 0.001 .The results emphasized the significance of the photoperiod.

Keywords

Trout, ELISA, Hormone, Melatonin, Pearl mullet

Van Yöresinde Yetişen Van Gölü Balığı İnci Kefali ve Alabalıkta Melatonin Hormonun Ölçülmesi

Abstract

B u çalışmada, Van Yöresinde yetişen Van Gölü balığı inci kefali ve alabalıkta melatonin hormonu seviyesi ölçüldü. Araştırma materyali olarak da Van Gölü’nde yetişen Van Gölü balığı inci kefali ve özel tesislerde yetiştirilen alabalık kullanıldı. Araştırma, insanlar gibi bu hormonun salgılandığı hayvanlar olan balıklarda; gelişim ve stres hormonu olan melatoninin bu özelliği göz önünde bulundurarak yürütülmüştür. Metod; melatonin hormonu, canlılardan Van Gölü balığı ve alabalıkta alınan kanda, özel kitlerle elisa cihazı ile ölçüldü. Araştırma, alabalık ve Van Gölü balığının plazmasında melatonin ölçülmesinden sonra, elisa parelelizmi standartlarına uygun olarak yapıldı. Alabalıkta yapılan çalışmada ortalama değer; 160.8 ± 4.090pg/ml aydınlık , 198.3 ± 3.694 pg/ml. karanlık olarak ilkbaharda bulunmuştur. Kış mevsimi ölçümlerimizde ortalama değer ise 86.69 ± 3.203 pg/ml aydınlık 128.9 ± 1.999 pg/ml karanlık olarak bulunmuştur. Van Gölü balığında ise ortalama 84.73 ± 2.281 pg/ml. aydınlık , 118.1 ± 2.560 pg/ml. karanlık olarak ilkbahar ölçümleri bulunmuştur. Kış mevsimi ölçümlerimizde ise ortalama 36.32 ± 1.627 pg/ml aydınlık , 70.53 ± 2.919 pg/ml karanlık değeri bulunmuştur

Keywords

Alabalık, Elisa, Hormon, Melatonin, Van Gölü balığı.

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