Determination of Ochratoxin A in Grape Wines after Dispersive Liquid–Liquid Microextraction Using High Performance Thin Layer and Liquid Chromatography– Fluorescence Detection

Year 2012, Volume: 40 Issue: 2, 155 - 163, 01.04.2012

Hayriye Mine Antep Melek Merdivan

Abstract

In this study, a method was developed for analyzing ochratoxin A in wines using dispersive liquid–liquid microextraction combined with thin layer and liquid chromatography. Parameters such as type and volume of extraction solvent and dispersive solvent, extraction time and effect of salt were optimized to obtain the best extraction result. The linearity of response was employed in the concentration range of ochratoxin A in wines from 0.03-1.00 μg L-1. Under the optimum conditions, the extraction recovery as 63.9% and the enrichment factor as 34.5 were established.

Keywords

Ochratoxin A, wine, dispersive liquid liquid microextraction, TLC, HPLC

Türk Üzüm Şaraplarındaki Okratoksin A’ Nın Dispersif Sıvı-Sıvı Mikroekstraksiyonu Sonrası Yüksek Performanslı İnce Tabaka ve Sıvı Kromatografisi- Floresans Yöntemiyle Tayini

Abstract

B u çalışmada, kırmızı ve beyaz üzüm şaraplarında okratoksin A analizine yönelik dispersif sıvı-sıvı mikroekstraksiyon yöntemi geliştirildi. En iyi ekstraksiyon verimini elde etmek için ekstraksiyon çözücüsü ve dispersif çözücünün türü ve hacmi, ekstraksiyon zamanı, tuz etkisi gibi parametrelerin optimizasyonu gerçekleştirildi. Yöntemin 0.03-1.00 µg L-1 derişim aralığında doğrusal olduğu belirlenmiştir. Optimum koşullar altında, ekstraksiyon geri kazanımı % 63.9 ve zenginleştirme faktörü 34.5 olarak belirlendi

Keywords

Okratoksin A, şarap, dispersif sıvı-sıvı mikroekstraksiyonu, TLC ve HPLC

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