Toxicity of Individual/Combined Effect of Zn+2, Fe+2, Cu+2 to Synechocystis sp. E35 Isolated from Kucukcekmece Lagoon, Istanbul

Year 2012, Volume: 40 Issue: 3, 227 - 231, 01.08.2012

Sevgi Demirel Belma Aslim Beyza Ustun Ebru Sebnem Yilmaz

Abstract

Iron, copper and zinc at toxic levels were found in water and sediment structure of Kucukcekmece Lake. In the research area, algae blooms have been observed dependent upon anthropogenic and abiotic factors at certain periods. Cyanobacteria species have been observed as dominant microorganism during algae bloom. The objective of the present study is to evaluate the toxic effects of various heavy metals on the Synechocystis sp. E35 isolated from Kucukcekmece Lagoon, Istanbul. Toxicity of Zn+2, Fe+2, Cu+2 and of metal mixture Zn+2- Cu+2, Zn+2-Fe+2, Fe+2-Cu+2 and Fe+2-Cu+2-Zn+2 are investigated in the laboratory scale. The order of toxicity of the metals to the Synechocystis sp. E35 is Cu+2> Zn+2>Fe+2 in growth media. The EC50 values of the Zn+2, Fe+2, Cu+2 on the inhibition of the growth of E35 isolate: are 6.94, 13.92 and 3.42 mg/l, respectively. Besides, combination of ternary metal systems showed stronger inhibitory effect on cells are 0.02 and 2.93 mg/l, respectively.

Keywords

Cyanobacteria, Kucukcekmece Lagoon, Heavy metal

İstanbul Küçükçekmece Gölünden İzole Edilen Synechocystis sp. E35 üzerine Zn+2, Fe+2 ve Cu+2 Metalerinin Tekli /Kombine Bileşiklerinin Toksisitesi

Abstract

K üçükçekmece gölünün su ve sediment yapısı toksik düzeyde demir,bakır ve çinko bulundurur. Araştırma alanında, belli dönemlerde antropojenik ve abiyotik faktörlere bağlı olarak yoğun alg üremeleri gözlenmiştir. Bunlar içerisinde siyanobakterilerin dominant grup olduğu tespit edilmiştir. Bu çalışmanın amacı, Küçükçekmece Gölünden izole edilen Synechocystis sp. E35 izolatı üzerine çeşitli ağır metallerin toksik etkisinin incelenmesidir. Laboratuar ölçeğinde, Zn+2, Fe+2, Cu+2 ve metal karışımlarının Zn+2-Cu+2, Zn+2-Fe+2, Fe+2-Cu+2 ve Fe+2-Cu+2-Zn+2 etkisi araştırılmıştır. Tekli uygulamalarda metallerin toksisiteleri sırasıyla Cu+2>Zn+2>Fe+2’dır. Metallerin ECmetal kombinasyon uygulamalarının da güçlü inhibitör etki gösterdiği tespit edilmiştir EC10= 0.02 mg/l ; EC50 =2.93 mg/l

Keywords

Siyanobakteri, Küçükçekmece Lagünü, Ağır metal

References

• 1. MG. Dominguez, LL. Maury, FJ. Florencio, JC. Reyes, A gene cluter involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803, J Bacteriol., 182 (2000) 1507.
• 2. K. Hantke, Bacterial zinc uptake and regulators, Curr. Microbiol., 8 (2005) 196.
• 3. DJB. Dalzell, S. Alte, E. Aspichueta, A. Sota, J. Etxebarria, M. Gutierrez, CC. Hoffmann, D. Sales, U. Obst, N. Christofi, A comparison of five rapid direct toxicity assessment methods to determine toxicity of pollutants to activated sludge, Chemosphere, 47 (2002) 535.
• 4. CD. Boswell, RE. Dick, LE. Macaskie, The effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of Acinetobacter johnsonii. Microbiology, 145 (1999) 1711.
• 5. B. Ustun, N. Ince, G. Cansever, E. Ulger, B. Agcioglu, Z. Sapçı, S. Demirel, E. Okumus, The Development of Environmental Management Model in Küçükçekmece Basin, Joint Research and Development Project (GSRT-TUBITAK) Project No: 102Y011 (2005).
• 6. S. Demirel, ZS. Zengin, B. Ustun, Determination of Phytotoxicity Effect in Polluted Sediment: In Case “Kucukcekmece Lagoon and Sazlidere Creek”, International Conference on Environmental Science and Technology, Houston, Texas, USA. Proceedings, 2 (2007) 201.
• 7. S. Demirel, Effect of physicochemical conditions on heavy metal biosorption in natural environments, PhD Thesis, Yıldız Technical University, Istanbul (2008).
• 8. RW. Castenholz, Phylum BX. Cyanobacteria. Oxygenic Photosynthetic Bacteria”, In: Bergey‘s Manual of Systematic Bacteriology, Volume 1: The Archaea and the Deeply Branching and Phototropic Bacteria 2nd Ed., G. Garrity, DR. Boone, and RW. Castenholz, Springer-Verlag, New York, 474 (2001).
• 9. R. Rippka, J. Deruelles, J. Waterbury, M. Herdman, R. Stanier, Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria, J. Gen. Microbiol., 111 (1979) 1.
• 10. U. Nubel, F. Garcia-Pichel, G. Muyzer, PCR primers to amplify 16S rRNA genes from cyanobacteria, Appl. Environ. Microb., 63 (1997) 3327.
• 11. J. Myers, WA. Kratz, Relation Between Pigment Content And Photosynthetic characteristics in a Blue-Green Alga, J. Gen. Physiol., 39 (1955) 11.
• 12. DJ. Finney, Probit Analysis (rev. ed.), Cambridge Univ. Press, London,1963
• 13. E. Zak, B. Norling, R. Maitra, F. Huang, HB. Andersson Pakrasi, The initial steps of biogenesis of cyanobacterial photosystems occur in plasma membrane. Proceedings of the National Academy of Sciences, 98 (2001) 13443.
• 14. W. Surosz, KA. Palinska, Effects of Heavy-Metal Stress on Cyanobacterium Anabaena flos-aquae, Arch of Environ Con Tox., 48 (2004) 40.