Investigation of Applicability Instead of Chemical Disinfectants of Euphorbia orientalis L. Extracts

Year 2013, Volume: 41 Issue: 2, 151 - 157, 01.06.2013

Gülçin Alp Avcı Emre Avci Dursun Ali Kose İsmail Hakkı Akçal

Abstract

In our study, disinfectant effects of Euphorbia orientalis L. were investigated that found as free in nature and collected from Corum/Turkey and environment. It was used three different soluble solutions ethanol, chloroform and water for extracts. Staphylococcus aureus ATCC 25923, Candida albicans ATCC 10231, Escherichia coli ATCC 25922, Enterecoccus faecalis ATCC 29212 and Pseudomonas aeroginosa ATCC 27853 strains used by disc diffusion method in determinate of antimicrobial activities of extracts. Water and ethanol extracts of plant were examined for antioxidant activities. And also, GC/MS analyses were carried out by chromatographic. On all microorganisms of ethanol and chloroform-plant extracts observed inhibition effects in different rates. The most sensitive Candida albicans strain and the most resistance strain Staphylococcus aureus were determinate in antimicrobial activity studies. An antioxidant activity of ethanol and water-plant extracts has observed in our studies. Antioxidant activities of water extract were higher than ethanol extract. At the same time, diphenic acid derivatives were found nearly the content of all extracts with GC/MS.

Keywords

Euphorbia orientalis L., chemical disinfectants, antimicrobial effect, antioxidant activity, GC/MS

Euphorbia Orientalis L. Özütlerinin Kimyasal Dezenfektanlar Yerine Uygulanabilirliğinin Araştırılması

Abstract

Ç alışmamızda, Çorum/Türkiye ve çevresinden toplanan ve doğada serbest olarak bulunan Euphorbia orientalis L.’in dezenfektan etkisi araştırıldı. Ekstraksiyonlar için üç farklı çözücü solüsyon etanol, kloroform ve su kullanıldı. Örneklerin antimikrobiyal aktivitesinin belirlenmesinde disk difüzyon yöntemi ile Staphylococcus aureus ATCC 25923, Candida albicans ATCC 10231, Escherichia coli ATCC 25922, Enterecoccus faecalis ATCC 29212 ve Pseudomonas aeroginosa ATCC 27853 suşları kullanıldı. Antioksidan aktivite için su ve etanol özütleri incelendi. Aynı zamanda kromotografi aracılığıyla GC/MS analizi yapıldı. Bitkinin etanol ve kloroform özütlerinin tüm mikroorganizmalar üzerinde farklı oranlarda inhibisyon etkisi gözlendi. Antimikrobiyal aktivite çalışmalarında en duyarlı suş C. albicans ve en dirençli suş S. aureus olarak belirlendi. Çalışmamızda bitkinin etanol ve su özütlerinin antioksidan aktiviteye sahip olduğu gözlendi. Bitkinin su özütlerinin antioksidan aktivitesinin etanol özütlerinden daha iyi olduğu belirlendi. Aynı zamanda, GC/MS ile hemen hemen tüm özütlerin içeriğinde difenik asit türevleri tespit edildi

Keywords

Euphorbia orientalis L., kimyasal dezenfektanlar, antimikrobiyal etki, antioksidan aktivite, GS/MS

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