Detection of Bt gene and seed purity in maize

Year 2013, Volume: 41 Issue: 4, 341 - 346, 01.11.2013

Shuang Yang Muhammad Irfan Xue Li Yan Bin Liu Xing Jia Sun

Abstract

In this study we have described the detection of Bt gene and seed purity in maize by using PCR based method. Fifty four maize varieties were used for the detection of Bt gene by using BT1 and 35S primer having size of 195bp and 301bp respectively. Of these, fifteen varieties contained Bt gene which means that these varieties can not be used for further breeding. Ninety six seeds of maize were used for purity check by bnlg 161 SSR primer. Results showed that these seeds were 95% pure with 5% impurity. So these results showed that PCR based markers were very helpful in cultivar identification which leads to the improvement in maize breeding programs.

Keywords

Bt gene, seed purity, PCR, maize

Mısırda Bt geninin ve tohum saflığının tayini

Abstract

Sunulan bu çalışmada, mısırda Bt geninin ve tohum saflığının PCR temelli yöntemle tayini sunulmuştur. Elli dört farklı mısır türü, 1950 bazçifti ve 301 bazçifti boyutuna sahip BT1 ve 35S primerleri kullanılarak Bt geninin tayini gerçekleştirilmiştir. Örnekler arasındaki onbeş örnek Bt geni içermektedir. Bt geni içermesi daha sonraki üreme işlemlerinde kullanılamayacağı anlamına gelmektedir. Doksanaltı mısır tohumu, bnIg 161 SSR primeri ile saflık kontrolu için kullanılmıştır. Sonuçlar, bu tohumların % 5 safsızlık ile % 95 saflıkta olduğunu göstermiştir. Ayrıca, bu sonuçlar PCR temelli işaretçilerin kültür tanımlanmasında çok faydalı olduğunu ve mısır yetiştirme programlarında iyileştirmeye olanak sağlayacağını göstermektedir

Keywords

Bt geni, tohum saflığı, PCR, mısır

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