Radiation synthesized acrylamide hydrogel: Preparation, characterization and usability as biomaterial

Year 2014, Volume: 42 Issue: 1, 129 - 141, 01.03.2014

Dursun Saraydın Serpil Ünver Saraydın Erdener Karadağ Olgun Güven

Abstract

Acrylamide hydrogel was prepared by γ-irradiating ofthe aqueous solution of acrylamide monomer with 4.65 kGy γ-rays. Spectroscopic, thermal and mechanical properties, swelling properties, diffusional behavior of water, diffusion coefficients and network properties of AAm hydrogel are examined. In vitro swelling and in vivo biocompatibility of acrylamide hydrogel were investigated. The swellings of AAm hydrogelare investigated in distilled water, human serum and some simulated physiological fluids such as phosphate buffer at pH 7.4, glycine-HCl buffer at pH 1.1 physiological saline solution. For the analysis of human sera biocompatibility, acrylamide hydrogel was incubated in 10 different human sera for 24 hours and its biocompatibility with some biochemical parameters have been investigated. No significant difference in values before and after the test procedures has been found. AAm hydrogel was subcutaneously implanted in rats for up to 10 weeks and the tissue response to these implants was studied. Histological analysis indicated that tissue reaction at the implant site progressed from an initial acute inflammatory response characterized. No necrosis, tumorigenesis or infection was observed at the implant site up to 10 weeks. In vivo studies indicated that the radiation induced acrylamide hydrogel was found to be well-tolerated, non-toxic and highly biocompatible.

Keywords

acrylamide, Hydrogel, biomaterial, biocompatibility

Radyasyonla sentezlenen akrilamid hidrojeli: Hazırlama, karakterizasyon ve biyomateryal olarak kullanılabilirliği

Abstract

A krilamidin sulu çözeltisi 4.65 kGy dozunda γ-ışınları ışınlanarak akrilamid AAm hidrojeli hazırlandı. AAm hidrojelinin spektroskopik, ısıl, mekanik özellikleri, şişme özellikleri, difüzyon davranışları, difüzyon katsayıları ve ağ özellikleri bulunmuştur.Amm hidrojelininin vitro şişmesi damıtık su, insan serumu ve fosfat tamponu pH 7.4 , fizyolojik serum çözeltisi, glisin - HCl tamponu pH 1.1 gibi bazı yapay fizyolojik sıvılarda incelenmiştir. İnsan serumunun bazı biyokimyasal parametreleri ile akrilamid hidrojelinin in vitro biyouyumluluk analizi için, AAm hidrojeli ile 10 farklı insan serumu 24 saat etkileştirilmiştir. Denemeler öncesi ve sonrası biyokimyasal parametrelerinin sayısal değerleri açısından anlamlı bir fark bulunmamıştır. AAm hidrojelinin in vivo biyouyumluluğu için, AAm hidrojeli sıçanları karın derialtı dokusuna yerleştirildi. Bu implantların doku yanıtı 10 hafta boyunca incelendi. İmplant bölgesinde nekroz, tümörogenez ya da enfeksiyon 10 hafta boyunca gözlenmemiştir. İn vivo çalışmalar, radyasyonla sentezlenen akrilamid hidrojelinin iyi tolere edilebildiği, toksik olmadığı ve biyouyumlu olduğu bulunmuştur

Keywords

Akrilamid, hidrojel, biyomateryal, biyouyumluluk

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