This study was performed to determine the effects of Aloe vera gel AVG from the leaves of Aloe barbadensis which is commercially available product in the market for human use, on male reproductive tract of Swiss albino mice. Male mice aged 4 weeks separated into four groups randomly received AVG by gavage daily for 28 days. The groups were as follows n=5 : control, ×0.5 dose group received half of recommended AVG dose, ×1 dose group received recommended AVG dose, and ×2 dose group received twice of recommended AVG dose. LH levels of mice in treatment groups decreased compared to control. Testosterone T levels decreased in ×2 dose group compared to control. Sperm head count did not change and sperm shape abnormalities in high dose group increased. Histo- pathological analysis showed that there were atrophic tubules, germ cell debris, picnotic cells and Sertoli cell vacu- olization in testes of high dose groups. In prostate glands, atrophic tubules and mononuclear cell infiltration were observed. There were no changes in malondialdehyde and reduced glutathione levels of testes among groups. The findings of this study indicate that oral usage of AVG may cause adverse effects on the male reproductive tract of mice by affecting the secretion of reproductive hormones.
A loe barbadensis’in yapraklarından üretilen ve ticari olarak insanların kullanımı için marketlerde satılan Aloa vera’ jelin AVG erkek Swiss albino farelerin üreme sistemi üzerindeki etkileri açığa çıkartılmaya çalışılmıştır. 4 haftalık erkek fareler rastgele dört gruba ayrılmış ve 28 gün süreyle mide yoluyla AVG verilmiştir. Gruplar kontrol n=5 , önerilen AVG dozun yarısının verildiği x0.5 grubu, önerilen AVG dozun verildiği x1grubu, önerilen AVG dozun iki katının verildiği x2 grubu şeklindedir. Uygulama gruplarında LH seviyeleri kontrol grubu ile karşılaştırıldığında azalmıştır. Testosteron T seviyeleri kontrol grubu ile karşılaştırıldığında x2 doz grubunda azalmıştır. Sperm başı sayımı değişmez iken sperm anomalileri yüksek doz grubunda artmıştır. Histopatolojik analizlerde yüksek doz gruplarında testisde Sertoli hücre vakuolizasyonu, piknotik hücreler, germ hücre atıkları, atropik tübüller gözlenmiştir. Prostat bezinde atropik tübüller ve mononüklear hücre infilitrasyonu gözlenmiştir. Testisde malondialdehit ve redükte glutatyon seviyelerinde gruplar arasında fark görülmemiştir. Çalışmanın bulgularından elde edilen sonuç ağızdan alınan AVG’nin hormonların salınımını etkileyerek farelerin üreme sisteminde zararlı etkilere neden olabilir
Aslı Korkmaz
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Ministry of Agriculture and Rural Affairs, National Food Reference Laboratory, Fatih Sultan Mehmet Avenue, Yenimahalle, Ankara, Turkey
Nurhayat Barlas
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Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey
İlhan Gürbüz
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Faculty of Pharmacy, Department of Pharmacognosy, Gazi University, Ankara, Turkey
İsmet Çok
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Faculty of Pharmacy, Department of Pharmacognosy, Gazi University, Ankara, Turkey
Ahbab, M. A., Korkmaz, A., Barlas, N., Gürbüz, İ., et al. (2014). Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product. Hacettepe Journal of Biology and Chemistry, 42(3), 351-360.
AMA
Ahbab MA, Korkmaz A, Barlas N, Gürbüz İ, Çok İ. Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product. HJBC. September 2014;42(3):351-360.
Chicago
Ahbab, Müfide Aydoğan, Aslı Korkmaz, Nurhayat Barlas, İlhan Gürbüz, and İsmet Çok. “Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product”. Hacettepe Journal of Biology and Chemistry 42, no. 3 (September 2014): 351-60.
EndNote
Ahbab MA, Korkmaz A, Barlas N, Gürbüz İ, Çok İ (September 1, 2014) Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product. Hacettepe Journal of Biology and Chemistry 42 3 351–360.
IEEE
M. A. Ahbab, A. Korkmaz, N. Barlas, İ. Gürbüz, and İ. Çok, “Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product”, HJBC, vol. 42, no. 3, pp. 351–360, 2014.
ISNAD
Ahbab, Müfide Aydoğan et al. “Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product”. Hacettepe Journal of Biology and Chemistry 42/3 (September 2014), 351-360.
JAMA
Ahbab MA, Korkmaz A, Barlas N, Gürbüz İ, Çok İ. Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product. HJBC. 2014;42:351–360.
MLA
Ahbab, Müfide Aydoğan et al. “Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product”. Hacettepe Journal of Biology and Chemistry, vol. 42, no. 3, 2014, pp. 351-60.
Vancouver
Ahbab MA, Korkmaz A, Barlas N, Gürbüz İ, Çok İ. Biochemical and Histological Alterations In Reproductive Tract Tissues of Male Swiss Albino Mice Exposed Commercially Prepared Aloe Vera Gel Product. HJBC. 2014;42(3):351-60.