Determination of Total Phenolic Compounds, Antioxidant Activity and Ferulic Acid in Extracts of Sugar Beet Pulp

Year 2015, Volume: 43 Issue: 4, 251 - 257, 01.11.2015

Aazam Aarabi Masoud Honarvar Maryam Mizani Hossein Faghihian

Abstract

In the resent research, two extracts methanol and NaOH 0.5 molL-1 were analyzed for the total phenolic contents TPC , ferulic acid concentration and antioxidant activity. Results revealed that significant differences were found in TPC among different extracts and alkaline extracts contained higher amounts of polyphenols than methanolic extract. The ferulic acid concentration in the solvent and alkaline liquor varied according to extraction time and there were no significant differences between the ferulic acid concentration for all methanolic treatments. Antioxidant capacity for extracts were shown that the significant differences were found at a level of p < 0.05 between all extracts and correlation coefficient r2 = 0.82 showed that total phenolic content was responsible for antiradical efficiency in sugar beet pulp extracts. Our results strongly suggest sugar beet pulp extracts can be promising sources of potential antioxidants that can be used as additive in food industry.

Keywords

antioxidant activity, phenolic compounds, ferulic acid, sugar beet pulp

Şeker Pancarı Küspesi Özütünde Toplam Fenolik Bileşikler, Antioksidant Aktivite ve Ferulik Asit Tayini

Abstract

B u araştırmada iki özütün [metanol ve NaOH 0. 5 molL-1 ], toplam fenolik içeriği, ferulik asit derişimi ve antioksidan aktivitesi analiz edildi. Sonuçlarda TFİ de fenolik miktarın metanolik miktara göre daha fazla olduğu alkalin ve farklı ekstraklar arasında önemli farklılıklar olduğu görüldü. Solvent ve alkali likörlerindeki ferulik asit derişimleri ekstraksiyon zamanına göre çeşitlendirildiğinde, tüm metanolik tedavilerde ferulik asit konsantrasyonları arasında önemli bir fark olmadığı gözlendi. Ekstratlar için antioksidan kapasite, şeker pancarı küspesi ekstraktında antiradikal etki için sorumlu toplam fenolik içerik gösteren korelasyon sayısı r2= 0.82 ve tüm ekstratlar arasında p < 0.05 seviyesinde bulunan önemli farklılıklarla gösterildi. Sonuçlarımız ışığında şeker pancarı küspesi ekstraklarının gıda endüstirisinde katkı olarak kullanılabileceği ve potansiyel bir antioksidan kaynağı olabilecği görüldü

Keywords

Antioksidan aktivite, fenolik bileşikler, ferulik asit, şeker pancarı küspesi

References

• 1. E. Middleton, C. Kandaswami, T.C. Theoharides, The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease and cancer. Pharmacol Rev, 52 (2000) 673.
• 2. K. Alpinar, M. Ozyurek, U. Kolak, K. Guclu, C. Aras, M. Altun, S.E. Celik, K.I. Berker, B. Bektasoglu, R. Apak, Antioxidant Capacities of Some Food Plants Wildly Grown in Ayvalik of Turkey”. Food. Sci. Tech. Res., 15 (2009) 59.
• 3. A. Eghdami, F.Sadeghi, Determination of total phenolic and flavonoids contents in methanolic and aqueous extract of Achillea Millefolium. Org. Chem. J., 2 (2010) 81.
• 4. A. Tilay, M. Bule, J. Kishenkumar, U. Annapure, preparation of ferulic acid from agricultural wastes: its improved extraction and purification. J. Agric. Food. Chem., 56 (2008) 7644.
• 5. S.B. Ghatak, S.J. Panchal, Ferulic acid–an insight into its current research and future prospects. Trends in Food Science & Technology, In Press, Accepted Manuscript, Available online 13 November (2010).
• 6. H.Z. Zhao, M.H. Moghadasian, Chemistry, natural sources, dietary intake and pharmacokinetic properties of ferulic acid: A review, Food Chem., 109 (2008) 691.
• 7. G.H. Lu, K. Chan, K. Leung, C.L. Chan, Z.H. Zhao, Z.H. Jiang, Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis. J. Chromatogr. A, 1068 ( 2005) 209.
• 8. M. Bunzel, J. Ralph, C. Funk, H. Steinhart, Isolation and identification of a ferulic acid dehydrotrimer from saponified maize bran insoluble fiber. Eur. Food. Res. Technol., 217 (2003) 128.
• 9. N. Turkmen, F. Sari, Y.S. Velioglu, The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food. Chem., 93 (2005) 713.
• 10. M.E. Alanon, L. Castro-Vazquez, M.C. Diaz-Maroto, M.H. Gordon, M.S. Pérez-Coello, A study of the antioxidant capacity of oak wood used in wine ageing and the correlation with polyphenol composition. Food. Chem., 128 (2011) 997.
• 11. P. Torre, B. Aliakbarian, B. Rivas, J.M. Dominguez, A.A. Converti, Release of ferulic acid from corn cobs by alkaline hydrolysis. Biochem. Eng. J., 40 (2008) 500.
• 12. M.S. Noor Hasyierah, M.D. Mohamed Zulkali, A. Dachyar, A. Muhammad Syarhabil, K.I. Ku Syahidah, Optimization of alkaline hydrolysis of paddy straw for ferulic acid extraction. Ind. Crop. Pro., 34 (2011) 1635.
• 13. C.D. Stalikas, Extraction, separation and detection methods for phenolic acids and flavonoids. J. Sep. Sci., 30 (2007) 3268.
• 14. D.S. Oufnac, Xu, Z. Sun, T. Sabliov, C. Prinyawiwatkul, W. Godber, J.S. Extraction of antioxidants from wheat bran using conventional solvent and microwaveassisted methods. Cereal. Chem., 84 (2007) 125–129.
• 15. A.M. Pisoschi, and G.P. Negulescu, Methods for total antioxidant activity determination: a review. Biochem. Anal. Biochem., 1 (2011) 1.
• 16. Y. Sun, W. Wenhua Wang, Ultrasonic extraction of ferulic acid from ligusticum chuanxiong. J. Chin.Inst. Chem. Eng., 39 (2008) 653.
• 17. F. Xu, R.C. Sun, J.X. Sun, C.F. Liu, B.H. Heb, J.S. Fan, Determination of cell wall ferulic and p-coumaric acids in sugarcane bagasse, Anal. Chem. Acta., 552 (2005) 207.