Genetic Variation and Microevolution at Allozyme Loci in Eastern Mediterranean Populations of the Geometrid Moth Cyclophora Puppillaria Lepidoptera
Abstract
Genetic variation at 7 allozyme loci were determined in 4 Meditarranean populations of the moth Cyclophora puppillaria. Polymorphism levels, and gene diversities as Hardy-Weinberg heterozygosities are mostly substantial for the loci except for those in one of the samples. Pairwise genetic distances between the samples are expressed as FST values, and a high degree of differentiation exists for almost all of the pairwise comparisons. A test of natural selection, Ewens-Watterson test of neutrality, shows no sign of selective difference among electrophoretic allelic states over loci in all samples. Our results presents the the first picture of population genetic variation in this species and in accordance with the result of a previous DNA barcoding analysis pointing to low level of genetic variation.
References
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- R.C. Lewontin, Genetic basis of Evolutionary Change. Columbia University Press, NewYork & London, (1974).
- D.M. Hillis, C. Moritz, B.K. Mable, Molecular Systematics. Sunderland & Massachusetts: Sinauer Associates, (1996).
- A. Barbadilla, L.M. King, R.C. Lewontin, What does electrophoretic variation tell us about protein variation? Molecular Biology and Evolution, 13 (1996) 427-432.
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- M. Slatkin, An exact test for neutrality based on the Ewens sampling distribution. Genetical Research, 64 (1994) 71-74.
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- B. Weir, Genetic Data Analysis. Sinauer Association, Sunderland, MA, USA, (1990). R. Rougerie, E.J. Nieukerken, S. Ratnasingham, Hebert,P.D.N., Genetic patterns in European Geometrid moths revealed by the barcode Index number (BIN) system. PLoS ONE 8e84518, 1-11 (2013).
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Geometrid Bir Güve olan Cyclophora Puppilaria Lepidoptera ’nın Doğu Akdeniz Popülasyonlarındaki Allozim Lokuslardaki Genetik Varyasyon ve Mikroevrim
Abstract
C cylophora puppillaria’nın Akdeniz Bölgesi’nden örneklenen 4 popülasyonundaki 7 allozim lokusuna dayalı genetik varyasyonu ele almıştır. Hesaplanan polimorfizm düzeyleri, Hardy-Weinberg heterozigotluğu şeklindeki gen çeşitlilikleri popülasyonların biri dışında önemli büyüklüktedir. Popülasyonlar arası genetik uzaklıklar FST şeklinde ifade edilmiştir ve hemen tüm ikili karşılaştırmalar yüksek bir farklılaşma düzeyi olduğuna işaret etmektedir. Aynı zamanda, Ewens-Watterson nötralite testi, doğal seçilimin varlığını bulgulamak için kullanılmıştır ve test sonuçları bütün popülasyonlardaki tüm elektroforetik lokusların alelik durumları için seçilim önemli olmadığını göstermektedir. Elde ettiğimiz sonuçlar bu türe ilişkin ilk poülasyon genetik varyasyon profilini sunmaktadır ve daha önce gerçekleştirilen ve düşük düzeyli bir genetik varyasyon bulunduğunu ifade eden DNA barkodlama analizin sonucuyla da uyuşmaktadır
References
- B. Charlesworth, D. Charlesworth, Elements of Evolutionary Genetics. Roberts and Company 10. G.A. Watterson, The homozygosity test of neutrality. Publishers, Greenwood Village Colorado (2010).
- R.C. Lewontin, Genetic basis of Evolutionary Change. Columbia University Press, NewYork & London, (1974).
- D.M. Hillis, C. Moritz, B.K. Mable, Molecular Systematics. Sunderland & Massachusetts: Sinauer Associates, (1996).
- A. Barbadilla, L.M. King, R.C. Lewontin, What does electrophoretic variation tell us about protein variation? Molecular Biology and Evolution, 13 (1996) 427-432.
- A. Hausmann, The Geometrid Moths of Europe. 14. A. Hausmann, H.C.J. Godfray, P. Huemer, M. Mutanen, Volume 1. Apollo Books, Stenstrup Denmark, (2001).
- K. Mazzucco, Cosymbia pupillaria Hbn. Bei 3200 m. Z. Wien. Ent. Ges. 51 (1966) 154.
- A. Hausmann, Geometrid Moths of Europe. Volume 2. ApolloBooks, Stenstrup, Denmark, (2004).
- G.P. Manchenko, Handbook of Detection of Enzymes on Electrophoretic Gels. CRC Press, Vladivostok, Russia, (2003).
- W.J. Ewens, The sampling theory of selectively neutral alleles. Theoretical Population Biology, 3 (1972) 87–112. Genetics, 88 (1978) 405–417.
- M. Slatkin, An exact test for neutrality based on the Ewens sampling distribution. Genetical Research, 64 (1994) 71-74.
- L. Excoffier, G. Laval, S. Schneider, Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1 (2005) 47-50.
- B. Weir, Genetic Data Analysis. Sinauer Association, Sunderland, MA, USA, (1990). R. Rougerie, E.J. Nieukerken, S. Ratnasingham, Hebert,P.D.N., Genetic patterns in European Geometrid moths revealed by the barcode Index number (BIN) system. PLoS ONE 8e84518, 1-11 (2013).
- J.A. Endler, Geographic Variation,Speciation, and Clines. Princeton University Press, Princeton (1977).
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2016 |
| Published in Issue | Year 2016 Volume: 44 Issue: 1 |
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HACETTEPE JOURNAL OF BIOLOGY AND CHEMİSTRY
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