Investigation of Antimicrobial Activity of Different Trametes versicolor Extracts on Some Clinical Isolates

Year 2016, Volume: 44 Issue: 3, 267 - 272, 01.09.2016

Erkay Özgör Meltem Ulusoy İrem Çelebier Semiha Selda Yıldız Nevin Keskin

Abstract

Intensive drug uses against species which have clinical significance and cause severe diseases have triggered the development of resistance in these species. It is necessary to discover new potential drugs against cli- nical isolates because many antimicrobial drugs become ineffective in the coming years. In this research, we used different extracts from Trametes versicolor fruiting bodies for investigating antibacterial and antifungal activities on some clinical isolates. For this purpose, five bacterial species Staphylococcus aureus, Entero- coccus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa and a fungal species Candida albicans were selected as clinically important isolates and four different crude extracts chloroform, water, ethyl acetate and ethyl alcohol of Trametes versicolor were tried on these species in terms of antimicrobial sensitivity with disc diffusion method. Obtained data were analyzed using SPSS 14 software. At the result, some of extracts were found to have antimicrobial activity on clinical isolates although all extracts generally show antimicrobial effect. Chloroform extract was found to have the highest antimicrobial activity on S. aureus and E. faecalis while ethanol extract was observed as more effective on P. aeruginosa, E. coli, B. subtilis and C. albicans isolates. It can therefore be suggested that, some of Trametes versicolor extracts are promising antimicrobial agents.

Keywords

Trametes versicolor, antimicrobial activity, antifungal activity, Clinical isolates

Farklı Trametes versicolor Özütlerinin Bazı Klinik İzolatlar Üzerindeki Antimikrobiyal Aktivitesinin İncelenmesi

Abstract

K linik önemi olan ve ciddi hastalıklara sebep olan türlere karşı yoğun ilaç kullanımı bu türlerde direnç gelişimini tetiklemektedir. İlerleyen yıllarda birçok antimikrobiyal ilaç etkisiz hale geleceğinden bu türlere karşı yeni potansiyel ilaçların keşfedilmesi gereklidir. Bu araştırmada, Trametes versicolor üreme yapılarından elde edilen farklı özütler, bazı klinik izolatlar üzerinde antibakteriyel ve antifungal aktiviteleri araştırmak için kullanılmıştır. Bu amaç doğrultusunda, klinik önemi olan izolatlar olarak 5 bakteri türü Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa ile bir fungus türü Candida albicans seçildi ve Trametes versicolor’un 4 farklı ham özütünün kloroform, su, etil asetat, etil alkol , bu türler üzerinde antimikrobiyal duyarlılık açısından disk difüzyon yöntemi kullanılarak denendi. Elde edilen veriler SPSS 14 programı kullanılarak analiz edildi. Çalışmanın sonunda, tüm özütler genel olarak antimikrobiyal etki gösterse de özütlerden bazılarının klinik izolatlar üzerinde antimikrobiyal aktiviteye sahip olduğu bulunmuştur. Kloroform özütünün S. aureus ve E. faecalis üzerinde en yüksek antimikrobiyal aktiviteye sahip olduğu bulunurken etanol özütünün P. aeruginosa, E. coli, B. subtilis ve C. albicans izolatları üzerinde daha etkili olduğu gözlenmiştir. Bu nedenle, bazı Trametes versicolor özütlerinin umut verici antimikrobiyal ajanlar olabileceği söylenebilir

Keywords

Trametes versicolor, Antimikrobiyal aktivite, Antifungal aktivite, Klinik izolatlar

References

• M. Corrado, K.F. Rodrigues, Antimicrobial evaluation of fungal extracts produced by endophytic strains of Phomopsis sp., Journal of Basic Microbiology, 44 (2004) 157-160.
• R. Pinner, S. Teutsch, L. Simonsen, L. Klug, J. Graber, M. Clarke, R. Berkelman, Trends in infectious diseases mortality in the united states, Journal of the American Medical Association, 275 (1996) 189-193.
• L. Hleba, N. Vukovi, J. Petrová, M. Kaániová, 13. A. Yamasaki, M. Shoda, H. Iijima, S. Nagai, J. Wada, Antimicrobial activity of crude methanolic extracts from Ganoderma lucidum and Trametes versicolor, Animal Science and Biotechnologies, 47 (2014) 89-93.
• M.S. Butler, The role of natural product chemistry in drug discovery, Journal of Natural Products, 67 (2004) 2141-2153.
• K.S. Lam, New aspects of natural products in drug discovery, Trends in Microbiology, 15 (2007) 6279- 6289.
• N. Sheena, T.A. Ajith, A.T. Mathew, K.K. Janardhanan, Antibacterial activity of three macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus occuring in South India, Pharmaceutical Biology, 41 (2003) 564-567.
• S. Quereshi, A.K. Pandey, S.S. Sandhu, Evaluation of antibaterial activity of different Ganoderma lucisum extracts, Journal of Scientific Research, 3 (2010) 9-13.
• Y. Gao, W. Tang, H. Gao, E. Chan, J. Lan, X. Li, S. Zhou, Antimicrobial activity of medical mushrooms Ganoderma, Food Reviews International, 21 (2005) 211-222.
• S.G. Jonathan, I.O. Fasidi, Antimicrobial activities of two nigerian edible macro-fungi Lycoperdon pusilum (bat. ex) and Lycoperdon giganteum (pers.), African Journal of Biomedical Research, 6 (2003) 85-90.
• M.J. Alves, I.C.F.R. Ferreira, J. Dias, V. Teixeira, A. Martins, M. Pintado, A review on antimicrobial activity of mushrooms (Basidiomycetes) extracts and isolated compounds, Planta Medica, 78 (2012) 1707- 1718.
• A. Yokoyama, Distribution of tetracyclic triterpenoids of lanostane group and sterols in higher fungi especially of the polyporacea and related families, Phytochemistry, 14 (1975) 487-497.
• S. Endo, Lipids of five species of Polyporacea, Tokyo Gakugei, 16, 1981. H. Suzuki, N. Chikazawa, T. Tasaka, C. Kameda, H. Tanaka, M. Ikebe, E. Jo, N. Sato, M. Nakamura, F. Sekine, T. Morisaki, M. Katano, A protein-bound polysaccharide, PSK, enhances tumor suppression induced by docetaxel in a gastric cancer xenograft model, Anticancer Research, 29 (2009) 843-50.
• K. Oba, S. Teramukai, M. Kobayashi, T. Matsui, Y. Kodera, J. Sakamoto, Efficacy of adjuvant immunochemotherapy with polysaccharide K for patients with curative resections of gastric cancer, Cancer Immunology and Immunotherapy, 56 (2007) 905-1011.
• S.Keypour, H. Riahi, M. F. Moradali, H. Rafati, Investigation of the Antibacterial Activity of a Chloroform Extract of Ling Zhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae), from Iran, International Journal of Medicinal Mushrooms, 10 (2008) 345-349.
• R. Balakumar, E. Sivaprakasam, D. Kavitha, S. Sridhar, J.S. Kumar, Antibacterial and antifungal activity of fruit bodies of Phellinus mushroom extract, International Journal of Biosciences, 1 (2011) 72-77.
• D. Kavitha, R. Balakumar, E. Sivaprakasam, S. Sridhar, J. Suresh, Antibacterial and antifungal potential of fruit body extracts from daldinia concentrica (bolton) cesati & de notaris, International journal of pharmaceutical sciences and research, 2 (2011) 2376- 2379.
• H. Zheng, Y. Maoqing, X. Liqiu, T. Wenjuan, L. Lian, Z. Guolin, Purification and characterization of an antibacterial protein from the cultured mycelia of Cordyceps sinensis, Wuhan University Journal of Natural Sciences, 11 (2006) 709-714.