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Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model

Year 2016, Volume: 44 Issue: 3, 283 - 292, 01.09.2016

Abstract

Although nowadays the treatment methods were improved, acute myocardial infarction MI and heart failure are the most important cardiovascular mortality and morbidity causes. In this study, mesenchymal stem cells MSCs collected from the bone marrow was injected into myocardial regeneration areas of the Wistar albino rats with modified ischemic heart model and this areas were investigated by using electrocardiographic ECG data and histopathological analysis. In stem cell implementation group, an increase in the amount of vessels and cells were observed. In this group, the amplitudes of P waves, QRS complex and T waves had no changes, whereas there was a significant reduction on T wave duration and heart beat rate reduced significantly p≤0,001 . S-T depression increased to normal levels was observed in reference and control groups more than in stem cell implementation group.

References

  • E.C. Perin. Stem cell therapy for cardiovascular disease, Tex. Heart Inst. J., 33 (2006) 204-208.
  • M.A. Pfeffer, M.C. Fishbein, P.J. Fletcher et al., Myocardial infarct size and ventricular function in rats, Circ. Res., 44 (1979) 503-512.
  • E. Akbay and M.A. Onur, A new modified myocardial infarction animal model, Journal-Cardiovascular Surgery, 1 (2013) 69-71.
  • W. Dai, S.L. Hale, B.J. Martin et al., Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: Short- and long-term effects, Circulation, 112 (2005) 214-223.
  • Y. Jiang, B.N. Jahagirdar, R.L. Reinhardt et al., Pluripotency of mesenchymal stem cells derived from adult marrow, Nature, 418 (2002) 41-49.
  • N. Nagaya, K. Kangawa, T. Itoh et al., Transplantation of mesenchymal stem cells improves cardiac function in a rat model of dilated cardiomyopathy, Circulation, (2005) 1128-1135.
  • M.F. Pittenger and B.J. Martin, Mesenchymal stem cells and their potential as cardiac therapeutics, Circ. Res., 95 (2004) 9-20.
  • M.F. Pittenger, A.M. Mackayi, S.C. Beck, Multilineage potential of adult human mesenchymal stem cells, Science, 284 (1999) 143-147.
  • C. Toma, M.F. Pittenger, K.S. Cahil et al., Human mesenchymal stem cells differentiate to a 23. D. Orlic, J.M. Hill, A.E. Arai, Stem cells for myocardial cardiomyocyte phenotype in the adult murine heart, Circulation, 105 (2002) 93-98.
  • J. Wang, R. Luo, X. Zhang X et al., Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction, J. Zhejiang Univ. Science B. 7 (2006) 641
  • Z. Xie, M. Singh, K. Singh, Differential regulation of matrix metalloproteinase-2 and -9 expression and activity in adult rat cardiac fibroblasts in response to interleukin-1 beta. J. Biol. Chem., 279 (2004) 39513-9.
  • Y.W. Yang, H. Bai, C.B. Wang et al., Experimental study on influence of bone marrow mesenchymal stem cells on activation and function of mouse perioneal macrophages, Zhonghua Xue Ye Xue Za Zhi, 29 (2008) 540-543.
  • E.C. Perin, The use of stem cell therapy for cardiovascular disease, Tex. Heart Inst. J., 32 (2005) 392.
  • S. Schenk, N. Mal, A. Finan et al., Monocyte chemotactic protein-3 is a myocardial mesenchymal stem cell homing factor, Stem Cells, 25 (2007) 245
  • N.S. Ye, J. Chen, G.A. Luo et al., Proteomic profiling of rat bone marrow mesenchymal stem cells induced by 5-azacytidine, Stem Cells Dev., 15 (2006) 665-676.
  • K.E. Porter, N.E. Turner, D.J. O’Regan et al., Tumor necrosis factor alpha induces human atrial myofibroblats proliferation, invasion and MMP-9 secretion: inhibition by simvastation, Cardiovascular Research, 64 (2004) 507-515.
  • S. Makino, K. Fukuda, S. Miyoshi et al., Cardiomyocytes can be generated from marrow stromal cells in vitro, The Journal of Clinical Investigation, 5 (1999) 697- regeneration, Circ. Res., 91 (2002) 1092-1102.
  • M. Hou, K. Yang, H. Zhang et al., Transplantation of mesenchymal stem cells from human bone marrow improves damaged heart function in rats, International Journal of Cardiology, 115 (2007) 220
  • J. Tang, Q. Xie, G. Pan et al., Mesenchymal stem cells participate in angiogenesis and improve heart function in rat model of myocardial ischemia with reperfusion, European Journal of Cardio-thoracic Surgery, 30 (2006) 353-361.
  • Y.L. Tang, Q. Zhao, Y.C. Zhang et al., Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium, Regul. Pept., 117 (2004) 3-1. 27. S. Tomita, R. Li, R.D. Weisel et al., Autologous
  • Transplantaion of Bone Marrow Cells Improves Damaged Heart Function, Circulation, 100 (1999) 256.
  • S. Tomita, D.A. Mickle, R.D. Weisel et al., Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation, J. Thorac. Cardiovasc. Surg., 123 J.S. Wang, D. Shum-Tim, J. Galipeau et al., Marrow stromal cells for cellular cardiomyoplasty feasibility and potential clinical advantages, J. Thorac. Cardiovasc., 120 (2000) 999-1005.

Modifiye Edilmiş Miyokardiyal Enfarktüs Rat Modelinden Sonra Hasarlı Kalp Fonksiyonlarının Kemik İliği Mezenşimal Kök Hücreler İle İyileştirilmesi

Year 2016, Volume: 44 Issue: 3, 283 - 292, 01.09.2016

Abstract

A kut miyokard infarktüsü ve kalp yetmezliği; tedavideki gelişmelere rağmen günümüzde kardiyovasküler mortalite ve morbiditenin en önemli nedenlerindendir. Bu çalışmada, modifiye edilmiş iskemik kalp modeli oluşturulan Wistar albino sıçanlarda, kemik iliğinden elde edilen mezenşimal kök hücreler ile rejenere olan miyokardiyal alanlar, elektrokardiyografik EKG ve histopatolojik analizler yapılarak gözlenmiştir. Kök hücre uygulama grubunda damar ve hücre sayısında diğer gruplara göre zamana bağlı olarak artış gözlenmiştir. Ayrıca bu grupta EKG verilerinden P dalgası, QRS kompleksi, T dalgası amplitüdleri diğer gruplara göre farklılık göstermezken, T dalgasının süresi ve kalp atım sayısında önemli derecede azalma gözlenmiştir p≤0,001 . Referans grubuna göre kontrol grubunda S-T çökmesinde artış gözlenirken, kök hücre uygulama grubunda azalma gözlenmiştir

References

  • E.C. Perin. Stem cell therapy for cardiovascular disease, Tex. Heart Inst. J., 33 (2006) 204-208.
  • M.A. Pfeffer, M.C. Fishbein, P.J. Fletcher et al., Myocardial infarct size and ventricular function in rats, Circ. Res., 44 (1979) 503-512.
  • E. Akbay and M.A. Onur, A new modified myocardial infarction animal model, Journal-Cardiovascular Surgery, 1 (2013) 69-71.
  • W. Dai, S.L. Hale, B.J. Martin et al., Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: Short- and long-term effects, Circulation, 112 (2005) 214-223.
  • Y. Jiang, B.N. Jahagirdar, R.L. Reinhardt et al., Pluripotency of mesenchymal stem cells derived from adult marrow, Nature, 418 (2002) 41-49.
  • N. Nagaya, K. Kangawa, T. Itoh et al., Transplantation of mesenchymal stem cells improves cardiac function in a rat model of dilated cardiomyopathy, Circulation, (2005) 1128-1135.
  • M.F. Pittenger and B.J. Martin, Mesenchymal stem cells and their potential as cardiac therapeutics, Circ. Res., 95 (2004) 9-20.
  • M.F. Pittenger, A.M. Mackayi, S.C. Beck, Multilineage potential of adult human mesenchymal stem cells, Science, 284 (1999) 143-147.
  • C. Toma, M.F. Pittenger, K.S. Cahil et al., Human mesenchymal stem cells differentiate to a 23. D. Orlic, J.M. Hill, A.E. Arai, Stem cells for myocardial cardiomyocyte phenotype in the adult murine heart, Circulation, 105 (2002) 93-98.
  • J. Wang, R. Luo, X. Zhang X et al., Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction, J. Zhejiang Univ. Science B. 7 (2006) 641
  • Z. Xie, M. Singh, K. Singh, Differential regulation of matrix metalloproteinase-2 and -9 expression and activity in adult rat cardiac fibroblasts in response to interleukin-1 beta. J. Biol. Chem., 279 (2004) 39513-9.
  • Y.W. Yang, H. Bai, C.B. Wang et al., Experimental study on influence of bone marrow mesenchymal stem cells on activation and function of mouse perioneal macrophages, Zhonghua Xue Ye Xue Za Zhi, 29 (2008) 540-543.
  • E.C. Perin, The use of stem cell therapy for cardiovascular disease, Tex. Heart Inst. J., 32 (2005) 392.
  • S. Schenk, N. Mal, A. Finan et al., Monocyte chemotactic protein-3 is a myocardial mesenchymal stem cell homing factor, Stem Cells, 25 (2007) 245
  • N.S. Ye, J. Chen, G.A. Luo et al., Proteomic profiling of rat bone marrow mesenchymal stem cells induced by 5-azacytidine, Stem Cells Dev., 15 (2006) 665-676.
  • K.E. Porter, N.E. Turner, D.J. O’Regan et al., Tumor necrosis factor alpha induces human atrial myofibroblats proliferation, invasion and MMP-9 secretion: inhibition by simvastation, Cardiovascular Research, 64 (2004) 507-515.
  • S. Makino, K. Fukuda, S. Miyoshi et al., Cardiomyocytes can be generated from marrow stromal cells in vitro, The Journal of Clinical Investigation, 5 (1999) 697- regeneration, Circ. Res., 91 (2002) 1092-1102.
  • M. Hou, K. Yang, H. Zhang et al., Transplantation of mesenchymal stem cells from human bone marrow improves damaged heart function in rats, International Journal of Cardiology, 115 (2007) 220
  • J. Tang, Q. Xie, G. Pan et al., Mesenchymal stem cells participate in angiogenesis and improve heart function in rat model of myocardial ischemia with reperfusion, European Journal of Cardio-thoracic Surgery, 30 (2006) 353-361.
  • Y.L. Tang, Q. Zhao, Y.C. Zhang et al., Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium, Regul. Pept., 117 (2004) 3-1. 27. S. Tomita, R. Li, R.D. Weisel et al., Autologous
  • Transplantaion of Bone Marrow Cells Improves Damaged Heart Function, Circulation, 100 (1999) 256.
  • S. Tomita, D.A. Mickle, R.D. Weisel et al., Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation, J. Thorac. Cardiovasc. Surg., 123 J.S. Wang, D. Shum-Tim, J. Galipeau et al., Marrow stromal cells for cellular cardiomyoplasty feasibility and potential clinical advantages, J. Thorac. Cardiovasc., 120 (2000) 999-1005.
There are 22 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Esin Akbay This is me

Handan Sevim This is me

Özer Aylin Gürpınar This is me

Serdar Günaydın This is me

Mehmet Ali Onur This is me

Publication Date September 1, 2016
Published in Issue Year 2016 Volume: 44 Issue: 3

Cite

APA Akbay, E., Sevim, H., Gürpınar, Ö. A., Günaydın, S., et al. (2016). Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model. Hacettepe Journal of Biology and Chemistry, 44(3), 283-292.
AMA Akbay E, Sevim H, Gürpınar ÖA, Günaydın S, Onur MA. Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model. HJBC. September 2016;44(3):283-292.
Chicago Akbay, Esin, Handan Sevim, Özer Aylin Gürpınar, Serdar Günaydın, and Mehmet Ali Onur. “Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function After Modified Myocardial Infarction Rat Model”. Hacettepe Journal of Biology and Chemistry 44, no. 3 (September 2016): 283-92.
EndNote Akbay E, Sevim H, Gürpınar ÖA, Günaydın S, Onur MA (September 1, 2016) Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model. Hacettepe Journal of Biology and Chemistry 44 3 283–292.
IEEE E. Akbay, H. Sevim, Ö. A. Gürpınar, S. Günaydın, and M. A. Onur, “Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model”, HJBC, vol. 44, no. 3, pp. 283–292, 2016.
ISNAD Akbay, Esin et al. “Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function After Modified Myocardial Infarction Rat Model”. Hacettepe Journal of Biology and Chemistry 44/3 (September 2016), 283-292.
JAMA Akbay E, Sevim H, Gürpınar ÖA, Günaydın S, Onur MA. Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model. HJBC. 2016;44:283–292.
MLA Akbay, Esin et al. “Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function After Modified Myocardial Infarction Rat Model”. Hacettepe Journal of Biology and Chemistry, vol. 44, no. 3, 2016, pp. 283-92.
Vancouver Akbay E, Sevim H, Gürpınar ÖA, Günaydın S, Onur MA. Bone Marrow Mesenchymal Stem Cells to Improve Damaged Heart Function after Modified Myocardial Infarction Rat Model. HJBC. 2016;44(3):283-92.

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