Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD

Year 2017, Volume: 45 Issue: 3, 411 - 416, 01.09.2017

Ebru Büyüktuncel Özlem Kalkan Emine Şahin

Abstract

This study aimed to develope a simple reversed-phase HPLC method for simultaneous determination of organic acids in natural and commercial orange juices. The chromatographic separation of these compounds was performed in a single run by using isocratic mobile phase consisting of 10 mM KH2PO4 pH=2.2 at room temperature, with flow rate of 1 mL.min-1 within 10 min on Inertsil ODS-4 C18 column 250 x 4.0 mm, 5μm . An ultraviolet absorption at 210 nm and 245 nm was monitored. Linearity of calibration, accuracy, precision were examined as parts of the method validation. It has been found that the levels of organic acids in natural juices were higher than in commercial juices.

Keywords

Determination of organic acids, orange juices, HPLC/DAD

Doğal ve Ticari Portakal Sularındaki Organik Asitlerin HPLC/DAD Yöntemiyle Tayini

Abstract

B u çalışma, doğal ve ticari portakal sularında, organik asitlerin eş zamanlı olarak tayini için basit bir ters faz HPLC yöntemi geliştirmeyi amaçlamıştır. Bileşiklerin kromatografik ayrımı izokratik olarak 10 mM KH2PO4 pH= 2.2 hareketli fazıyla, oda sıcaklığında, 1 mL.min-1 akış hızında, 10 dakika içinde ve Inertsil ODS4 C18 kolonda 250 x 4.0 mm, 5μm , gerçekleşmiştir. Ultraviyole absorpsiyon 210 ve 245 nm dalgaboyunda izlenmiştir. Doğrusallık, doğruluk, kesinlik gibi parametreler belirlenerek yöntem valide edilmiştir. Doğal meyve sularındaki organik asit seviyesinin, ticari meyve sularından daha yüksek olduğu bulunmuştur

Keywords

Organik asit tayini, portakal suları, HPLC/DAD

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